Variability of seizure-like activity in an in vitro model of epilepsy depends on the electrical recording method
Background: Hippocampal and cortical slice-based models are widely used to study seizures and epilepsy. Seizure detection and quantification are essential components for studying mechanisms of epilepsy and assessing therapeutic interventions. To obtain meaningful signals and maximize experimental th...
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doaj-3c5d90f587074421b1f665006776f4322020-12-09T06:25:24ZengElsevierHeliyon2405-84402020-11-01611e05587Variability of seizure-like activity in an in vitro model of epilepsy depends on the electrical recording methodShabnam Ghiasvand0Chris R. Dussourd1Jing Liu2Yu Song3Yevgeny Berdichevsky4Bioengineering Lehigh University, United StatesElectrical Engineering Lehigh University, United StatesElectrical Engineering Lehigh University, United StatesBioengineering Lehigh University, United StatesBioengineering Lehigh University, United States; Electrical Engineering Lehigh University, United States; Corresponding author.Background: Hippocampal and cortical slice-based models are widely used to study seizures and epilepsy. Seizure detection and quantification are essential components for studying mechanisms of epilepsy and assessing therapeutic interventions. To obtain meaningful signals and maximize experimental throughput, variability should be minimized. Some electrical recording methods require insertion of an electrode into neuronal tissue, change in slice chemical microenvironment, and transients in temperature and pH. These perturbations can cause acute and long-term alterations of the neuronal network which may be reflected in the variability of the recorded signal. New method: In this study we investigated the effect of experimental perturbations in three local field potential (LFP) recording methods including substrate micro-wires (s-MWs), multiple electrode arrays (MEAs), and inserted micro wire electrodes (i-MW). These methods enabled us to isolate effects of different perturbations. We used organotypic hippocampal slices (OHCs) as an in-vitro model of posttraumatic epilepsy. To investigate the effect of the disturbances caused by the recording method on the paroxysmal events, we introduced jitter analysis, which is sensitive to small differences in the seizure spike timing. Results: Medium replacement can introduce long-lasting perturbations. Electrode insertion increased variability on a shorter time scale. OHCs also underwent spontaneous state transitions characterized by transient increases in variability. Comparison with existing methods: This new method of seizure waveform analysis allows for more sensitive assessment of variability of ictal events than simply measuring seizure frequency and duration. Conclusion: We demonstrated that some of the variability in OHC recordings are due to experimental perturbations while some are spontaneous and independent of recording method.http://www.sciencedirect.com/science/article/pii/S2405844020324300EpilepsySeizure similarityLocal field potentialMicrowireMEAJitter |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Shabnam Ghiasvand Chris R. Dussourd Jing Liu Yu Song Yevgeny Berdichevsky |
spellingShingle |
Shabnam Ghiasvand Chris R. Dussourd Jing Liu Yu Song Yevgeny Berdichevsky Variability of seizure-like activity in an in vitro model of epilepsy depends on the electrical recording method Heliyon Epilepsy Seizure similarity Local field potential Microwire MEA Jitter |
author_facet |
Shabnam Ghiasvand Chris R. Dussourd Jing Liu Yu Song Yevgeny Berdichevsky |
author_sort |
Shabnam Ghiasvand |
title |
Variability of seizure-like activity in an in vitro model of epilepsy depends on the electrical recording method |
title_short |
Variability of seizure-like activity in an in vitro model of epilepsy depends on the electrical recording method |
title_full |
Variability of seizure-like activity in an in vitro model of epilepsy depends on the electrical recording method |
title_fullStr |
Variability of seizure-like activity in an in vitro model of epilepsy depends on the electrical recording method |
title_full_unstemmed |
Variability of seizure-like activity in an in vitro model of epilepsy depends on the electrical recording method |
title_sort |
variability of seizure-like activity in an in vitro model of epilepsy depends on the electrical recording method |
publisher |
Elsevier |
series |
Heliyon |
issn |
2405-8440 |
publishDate |
2020-11-01 |
description |
Background: Hippocampal and cortical slice-based models are widely used to study seizures and epilepsy. Seizure detection and quantification are essential components for studying mechanisms of epilepsy and assessing therapeutic interventions. To obtain meaningful signals and maximize experimental throughput, variability should be minimized. Some electrical recording methods require insertion of an electrode into neuronal tissue, change in slice chemical microenvironment, and transients in temperature and pH. These perturbations can cause acute and long-term alterations of the neuronal network which may be reflected in the variability of the recorded signal. New method: In this study we investigated the effect of experimental perturbations in three local field potential (LFP) recording methods including substrate micro-wires (s-MWs), multiple electrode arrays (MEAs), and inserted micro wire electrodes (i-MW). These methods enabled us to isolate effects of different perturbations. We used organotypic hippocampal slices (OHCs) as an in-vitro model of posttraumatic epilepsy. To investigate the effect of the disturbances caused by the recording method on the paroxysmal events, we introduced jitter analysis, which is sensitive to small differences in the seizure spike timing. Results: Medium replacement can introduce long-lasting perturbations. Electrode insertion increased variability on a shorter time scale. OHCs also underwent spontaneous state transitions characterized by transient increases in variability. Comparison with existing methods: This new method of seizure waveform analysis allows for more sensitive assessment of variability of ictal events than simply measuring seizure frequency and duration. Conclusion: We demonstrated that some of the variability in OHC recordings are due to experimental perturbations while some are spontaneous and independent of recording method. |
topic |
Epilepsy Seizure similarity Local field potential Microwire MEA Jitter |
url |
http://www.sciencedirect.com/science/article/pii/S2405844020324300 |
work_keys_str_mv |
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