Biotechnological Utilisation of Fusel Oil, A Food Industry By-Product - A Kinetic Model on Enzymatic Esterification of i-Amyl Alcohol and Oleic Acid by Candida antarctica Lipase B

Biotechnological Utilisation of Fusel Oil, A Food Industry By-Product - A Kinetic Model on Enzymatic Esterification of i-Amyl Alcohol and Oleic Acid by Candida antarctica Lipase B

Fusel oil is a by-product of distilleries, its main component is i-amyl alcohol, which can form ester compounds. Esterification of oleic acid and i-amyl alcohol by Candida antarctica lipase B (Novozym 435 preparation) in n-heptane solvent was studied in this work. Ping-pong bi-bi mechanism (inhibiti...

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Main Authors: László Gubicza, Erika Fehér, Katalin Bélafi-Bakó, Nándor Nemestóthy
Format: Article
Language:English
Published: University of Zagreb 2008-01-01
Series:Food Technology and Biotechnology
Subjects:
fusel oil
esterification
lipase
ping-pong bi-bi mechanism
Online Access:http://hrcak.srce.hr/file/34893
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spelling doaj-3c70d011893248db8be6b539cb625c562020-11-25T02:08:46ZengUniversity of ZagrebFood Technology and Biotechnology1330-98621334-26062008-01-014614450Biotechnological Utilisation of Fusel Oil, A Food Industry By-Product - A Kinetic Model on Enzymatic Esterification of i-Amyl Alcohol and Oleic Acid by Candida antarctica Lipase BLászló Gubicza0Erika Fehér1Katalin Bélafi-Bakó2Nándor Nemestóthy3Research Institute of Chemical and Process Engineering, Pannon University, Egyetem u. 2, HU-8200 Veszprém, HungaryResearch Institute of Chemical and Process Engineering, Pannon University, Egyetem u. 2, HU-8200 Veszprém, HungaryResearch Institute of Chemical and Process Engineering, Pannon University, Egyetem u. 2, HU-8200 Veszprém, HungaryResearch Institute of Chemical and Process Engineering, Pannon University, Egyetem u. 2, HU-8200 Veszprém, HungaryFusel oil is a by-product of distilleries, its main component is i-amyl alcohol, which can form ester compounds. Esterification of oleic acid and i-amyl alcohol by Candida antarctica lipase B (Novozym 435 preparation) in n-heptane solvent was studied in this work. Ping-pong bi-bi mechanism (inhibition phenomena taken into account) was applied as a complex kinetic model. The parameters of the model were determined by numerical methods. It was found that four-parameter model fitted well with the experimental results and described properly the enzymatic reaction.http://hrcak.srce.hr/file/34893fusel oilesterificationlipaseping-pong bi-bi mechanism
collection DOAJ
language English
format Article
sources DOAJ
author László Gubicza
Erika Fehér
Katalin Bélafi-Bakó
Nándor Nemestóthy
spellingShingle László Gubicza
Erika Fehér
Katalin Bélafi-Bakó
Nándor Nemestóthy
Biotechnological Utilisation of Fusel Oil, A Food Industry By-Product - A Kinetic Model on Enzymatic Esterification of i-Amyl Alcohol and Oleic Acid by Candida antarctica Lipase B
Food Technology and Biotechnology
fusel oil
esterification
lipase
ping-pong bi-bi mechanism
author_facet László Gubicza
Erika Fehér
Katalin Bélafi-Bakó
Nándor Nemestóthy
author_sort László Gubicza
title Biotechnological Utilisation of Fusel Oil, A Food Industry By-Product - A Kinetic Model on Enzymatic Esterification of i-Amyl Alcohol and Oleic Acid by Candida antarctica Lipase B
title_short Biotechnological Utilisation of Fusel Oil, A Food Industry By-Product - A Kinetic Model on Enzymatic Esterification of i-Amyl Alcohol and Oleic Acid by Candida antarctica Lipase B
title_full Biotechnological Utilisation of Fusel Oil, A Food Industry By-Product - A Kinetic Model on Enzymatic Esterification of i-Amyl Alcohol and Oleic Acid by Candida antarctica Lipase B
title_fullStr Biotechnological Utilisation of Fusel Oil, A Food Industry By-Product - A Kinetic Model on Enzymatic Esterification of i-Amyl Alcohol and Oleic Acid by Candida antarctica Lipase B
title_full_unstemmed Biotechnological Utilisation of Fusel Oil, A Food Industry By-Product - A Kinetic Model on Enzymatic Esterification of i-Amyl Alcohol and Oleic Acid by Candida antarctica Lipase B
title_sort biotechnological utilisation of fusel oil, a food industry by-product - a kinetic model on enzymatic esterification of i-amyl alcohol and oleic acid by candida antarctica lipase b
publisher University of Zagreb
series Food Technology and Biotechnology
issn 1330-9862
1334-2606
publishDate 2008-01-01
description Fusel oil is a by-product of distilleries, its main component is i-amyl alcohol, which can form ester compounds. Esterification of oleic acid and i-amyl alcohol by Candida antarctica lipase B (Novozym 435 preparation) in n-heptane solvent was studied in this work. Ping-pong bi-bi mechanism (inhibition phenomena taken into account) was applied as a complex kinetic model. The parameters of the model were determined by numerical methods. It was found that four-parameter model fitted well with the experimental results and described properly the enzymatic reaction.
topic fusel oil
esterification
lipase
ping-pong bi-bi mechanism
url http://hrcak.srce.hr/file/34893
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