Sequence Diversity in tRNA Gene Locus A-L among Iranian Isolates of Entamoeba Dispar

• Main Authors: M Rostami-Nejad, M Tajbakhsh, S Romani, Z Nochi, M Azimirad, E Nazemalhosseini-Mojarad, A Haghighi, MR Zali
• Format: Article
• Language: English
• Published: Tehran University of Medical Sciences 2012-02-01
• Series: Iranian Journal of Parasitology
• Subjects: Entamoeba Dispar; STRs; Locus A-L; Diversity; Iran
• Online Access: http://journals.tums.ac.ir/PdfMed.aspx?pdf_med=/upload_files/pdf/20385.pdf&manuscript_id=20385
• ISSN: 1735-7020; 2008-238X

Background: A number of methods for detecting diversity in Entamoeba have been described over the years. In the present study the genetic polymorphism of noncoding locus A-L was ana­lyzed using PCR and sequencing in order to clarify the genotypic differences among E. dispar isolates.Methods: A total of 28 E. dispar from patients with gastrointestinal symptoms were determined and the genomic DNA was extracted directly from stool. For genotype analysis; Locus A-L was amplified by PCR and PCR products were sequenced .The sequences obtained were edited ma­nually and aligned using Gene Runner software.Results: With sequencing of PCR products a reliable genetic diversity in size, number and posi­tion of the repeat units were observed among the Iranian E. dispar isolates in locus A-L gene. Se­quences showed variation in length from 448bp to 507bp and seven distinct types were identified.Conclusion: The genetic diversity of loci like A-L shows them to be suitable for epidemiological studies such as the characterization of the routes of transmission of these parasites in Iran.