Oral Intake of EPA:DHA 6:1 by Middle-Aged Rats for One Week Improves Age-Related Endothelial Dysfunction in Both the Femoral Artery and Vein: Role of Cyclooxygenases
In humans, aging is associated with endothelial dysfunction and an increased risk of venous thromboembolism. Although intake of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) at a ratio of 6:1 by old rats improved the endothelial dysfunction in arteries, the impact on veins remains uncle...
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doaj-3c842f089781463889a9b512b616cd582020-11-25T01:47:08ZengMDPI AGInternational Journal of Molecular Sciences1422-00672020-01-0121392010.3390/ijms21030920ijms21030920Oral Intake of EPA:DHA 6:1 by Middle-Aged Rats for One Week Improves Age-Related Endothelial Dysfunction in Both the Femoral Artery and Vein: Role of CyclooxygenasesSébastien Gaertner0Cyril Auger1Muhammad A. Farooq2Brigitte Pollet3Sonia Khemais-Benkhiat4Zahid R. Niazi5Sophie Schrevens6Sin-Hee Park7Florence Toti8Dominique Stephan9Valérie B. Schini-Kerth10INSERM (French National Institute of Health and Medical Research), UMR 1260, Regenerativ.e Nanomedicine (RNM), FMTS, 67000 Strasbourg, FranceINSERM (French National Institute of Health and Medical Research), UMR 1260, Regenerativ.e Nanomedicine (RNM), FMTS, 67000 Strasbourg, FranceINSERM (French National Institute of Health and Medical Research), UMR 1260, Regenerativ.e Nanomedicine (RNM), FMTS, 67000 Strasbourg, FranceUMR 7021, Laboratoire Bioimagerie et Pathologies, Faculté de Pharmacie, 67401 Illkirch, FranceINSERM (French National Institute of Health and Medical Research), UMR 1260, Regenerativ.e Nanomedicine (RNM), FMTS, 67000 Strasbourg, FranceINSERM (French National Institute of Health and Medical Research), UMR 1260, Regenerativ.e Nanomedicine (RNM), FMTS, 67000 Strasbourg, FranceINSERM (French National Institute of Health and Medical Research), UMR 1260, Regenerativ.e Nanomedicine (RNM), FMTS, 67000 Strasbourg, FranceINSERM (French National Institute of Health and Medical Research), UMR 1260, Regenerativ.e Nanomedicine (RNM), FMTS, 67000 Strasbourg, FranceINSERM (French National Institute of Health and Medical Research), UMR 1260, Regenerativ.e Nanomedicine (RNM), FMTS, 67000 Strasbourg, FranceINSERM (French National Institute of Health and Medical Research), UMR 1260, Regenerativ.e Nanomedicine (RNM), FMTS, 67000 Strasbourg, FranceINSERM (French National Institute of Health and Medical Research), UMR 1260, Regenerativ.e Nanomedicine (RNM), FMTS, 67000 Strasbourg, FranceIn humans, aging is associated with endothelial dysfunction and an increased risk of venous thromboembolism. Although intake of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) at a ratio of 6:1 by old rats improved the endothelial dysfunction in arteries, the impact on veins remains unclear. Eight-month-old male Wistar rats were either untreated or orally administered corn oil, EPA:DHA 1:1, or EPA:DHA 6:1 (500 mg/kg/d) for seven days. Vascular reactivity was studied by myography. In middle-aged femoral artery rings, acetylcholine caused a partial relaxation at low concentrations and a contractile response at high concentrations, whereas in the old femoral vein only a partial relaxation was observed. The EPA:DHA 6:1 treatment blunted the contractile response to acetylcholine in the middle-aged femoral artery and both EPA:DHA 6:1 and 1:1 increased the relaxation to acetylcholine in the old femoral vein. No such effects were observed with corn oil. Both the non-selective cyclooxygenase inhibitor indomethacin and the COX-1 inhibitor SC-560 increased the relaxation to acetylcholine in the middle-aged femoral artery whereas the COX-2 inhibitor NS-398 increased that in the middle-aged femoral vein. In conclusion, our results indicate that aging is associated with an endothelial dysfunction in the femoral artery and vein, which can be improved by EPA:DHA 6:1 treatment—most likely via a cyclooxygenase-dependent mechanism.https://www.mdpi.com/1422-0067/21/3/920endothelial dysfunctionagingomega-3 polyunsaturated fatty acidscyclooxygenasesvenous thrombosis |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Sébastien Gaertner Cyril Auger Muhammad A. Farooq Brigitte Pollet Sonia Khemais-Benkhiat Zahid R. Niazi Sophie Schrevens Sin-Hee Park Florence Toti Dominique Stephan Valérie B. Schini-Kerth |
spellingShingle |
Sébastien Gaertner Cyril Auger Muhammad A. Farooq Brigitte Pollet Sonia Khemais-Benkhiat Zahid R. Niazi Sophie Schrevens Sin-Hee Park Florence Toti Dominique Stephan Valérie B. Schini-Kerth Oral Intake of EPA:DHA 6:1 by Middle-Aged Rats for One Week Improves Age-Related Endothelial Dysfunction in Both the Femoral Artery and Vein: Role of Cyclooxygenases International Journal of Molecular Sciences endothelial dysfunction aging omega-3 polyunsaturated fatty acids cyclooxygenases venous thrombosis |
author_facet |
Sébastien Gaertner Cyril Auger Muhammad A. Farooq Brigitte Pollet Sonia Khemais-Benkhiat Zahid R. Niazi Sophie Schrevens Sin-Hee Park Florence Toti Dominique Stephan Valérie B. Schini-Kerth |
author_sort |
Sébastien Gaertner |
title |
Oral Intake of EPA:DHA 6:1 by Middle-Aged Rats for One Week Improves Age-Related Endothelial Dysfunction in Both the Femoral Artery and Vein: Role of Cyclooxygenases |
title_short |
Oral Intake of EPA:DHA 6:1 by Middle-Aged Rats for One Week Improves Age-Related Endothelial Dysfunction in Both the Femoral Artery and Vein: Role of Cyclooxygenases |
title_full |
Oral Intake of EPA:DHA 6:1 by Middle-Aged Rats for One Week Improves Age-Related Endothelial Dysfunction in Both the Femoral Artery and Vein: Role of Cyclooxygenases |
title_fullStr |
Oral Intake of EPA:DHA 6:1 by Middle-Aged Rats for One Week Improves Age-Related Endothelial Dysfunction in Both the Femoral Artery and Vein: Role of Cyclooxygenases |
title_full_unstemmed |
Oral Intake of EPA:DHA 6:1 by Middle-Aged Rats for One Week Improves Age-Related Endothelial Dysfunction in Both the Femoral Artery and Vein: Role of Cyclooxygenases |
title_sort |
oral intake of epa:dha 6:1 by middle-aged rats for one week improves age-related endothelial dysfunction in both the femoral artery and vein: role of cyclooxygenases |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1422-0067 |
publishDate |
2020-01-01 |
description |
In humans, aging is associated with endothelial dysfunction and an increased risk of venous thromboembolism. Although intake of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) at a ratio of 6:1 by old rats improved the endothelial dysfunction in arteries, the impact on veins remains unclear. Eight-month-old male Wistar rats were either untreated or orally administered corn oil, EPA:DHA 1:1, or EPA:DHA 6:1 (500 mg/kg/d) for seven days. Vascular reactivity was studied by myography. In middle-aged femoral artery rings, acetylcholine caused a partial relaxation at low concentrations and a contractile response at high concentrations, whereas in the old femoral vein only a partial relaxation was observed. The EPA:DHA 6:1 treatment blunted the contractile response to acetylcholine in the middle-aged femoral artery and both EPA:DHA 6:1 and 1:1 increased the relaxation to acetylcholine in the old femoral vein. No such effects were observed with corn oil. Both the non-selective cyclooxygenase inhibitor indomethacin and the COX-1 inhibitor SC-560 increased the relaxation to acetylcholine in the middle-aged femoral artery whereas the COX-2 inhibitor NS-398 increased that in the middle-aged femoral vein. In conclusion, our results indicate that aging is associated with an endothelial dysfunction in the femoral artery and vein, which can be improved by EPA:DHA 6:1 treatment—most likely via a cyclooxygenase-dependent mechanism. |
topic |
endothelial dysfunction aging omega-3 polyunsaturated fatty acids cyclooxygenases venous thrombosis |
url |
https://www.mdpi.com/1422-0067/21/3/920 |
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