Imbalance of Pro- and Anti-Angiogenic Factors Due to Maternal Vitamin D Deficiency Causes Renal Microvasculature Alterations Affecting the Adult Kidney Function
Vitamin D (Vit.D) is involved in cellular proliferation and differentiation and regulation of the renin gene, which are important aspects of nephrogenesis and quiescence of renal health in adulthood. This study evaluated the angiogenic mechanisms involved in long term renal disturbances induced by V...
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doaj-3c85f3ec488b4b36bbc7d52ac85d373a2020-11-24T21:34:18ZengMDPI AGNutrients2072-66432019-08-01118192910.3390/nu11081929nu11081929Imbalance of Pro- and Anti-Angiogenic Factors Due to Maternal Vitamin D Deficiency Causes Renal Microvasculature Alterations Affecting the Adult Kidney FunctionLucas Ferreira de Almeida0Heloísa Della Coletta Francescato1Jose Antunes-Rodrigues2Francisco Jose Albuquerque de Paula3Cleonice Giovanni Alves da Silva4Roberto Silva Costa5Terezila Machado Coimbra6Department of Physiology of Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, São Paulo 14049-900, BrazilDepartment of Physiology of Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, São Paulo 14049-900, BrazilDepartment of Physiology of Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, São Paulo 14049-900, BrazilDepartment of Internal Medicine of Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, São Paulo 14049-900, BrazilDepartment of Physiology of Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, São Paulo 14049-900, BrazilDepartment of Pathology of Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, São Paulo 14049-900, BrazilDepartment of Physiology of Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, São Paulo 14049-900, BrazilVitamin D (Vit.D) is involved in cellular proliferation and differentiation and regulation of the renin gene, which are important aspects of nephrogenesis and quiescence of renal health in adulthood. This study evaluated the angiogenic mechanisms involved in long term renal disturbances induced by Vit.D deficiency persistent in adulthood in rats. First-generation male Hannover offspring from mothers fed either a control diet (control group, CG) or Vit.D-deficient diet (Vit.D- group) were evaluated. Systolic blood pressure (SBP) was measured monthly during the first 6 months after birth, and blood and urine samples were collected to evaluate renal function. Nitric oxide (NO), angiotensin II (ANGII), parathyroid hormone (PTH), calcium, and Vit.D were measured. The kidneys were then removed for morphometric, NO, immunohistochemical, and Western blot studies. We evaluated the expression of vascular growth factor (VEGF) and angiopoietins 1 and 2 and their receptors since this intrinsic renal axis is responsible for endothelial quiescence. Compared to CG, the Vit.D- group presented higher SBP, ANG II plasma levels, renin expression, and AT1 receptor expression levels. Capillary rarefaction was observed, as well as an imbalance between pro- and anti-angiogenic factors. Collectively, the present findings support the role of Vit.D for maintaining the integrity of renal microcirculation.https://www.mdpi.com/2072-6643/11/8/1929angiogenesisrenin-angiotensin systemrenal microcirculationvitamin D deficiency |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Lucas Ferreira de Almeida Heloísa Della Coletta Francescato Jose Antunes-Rodrigues Francisco Jose Albuquerque de Paula Cleonice Giovanni Alves da Silva Roberto Silva Costa Terezila Machado Coimbra |
spellingShingle |
Lucas Ferreira de Almeida Heloísa Della Coletta Francescato Jose Antunes-Rodrigues Francisco Jose Albuquerque de Paula Cleonice Giovanni Alves da Silva Roberto Silva Costa Terezila Machado Coimbra Imbalance of Pro- and Anti-Angiogenic Factors Due to Maternal Vitamin D Deficiency Causes Renal Microvasculature Alterations Affecting the Adult Kidney Function Nutrients angiogenesis renin-angiotensin system renal microcirculation vitamin D deficiency |
author_facet |
Lucas Ferreira de Almeida Heloísa Della Coletta Francescato Jose Antunes-Rodrigues Francisco Jose Albuquerque de Paula Cleonice Giovanni Alves da Silva Roberto Silva Costa Terezila Machado Coimbra |
author_sort |
Lucas Ferreira de Almeida |
title |
Imbalance of Pro- and Anti-Angiogenic Factors Due to Maternal Vitamin D Deficiency Causes Renal Microvasculature Alterations Affecting the Adult Kidney Function |
title_short |
Imbalance of Pro- and Anti-Angiogenic Factors Due to Maternal Vitamin D Deficiency Causes Renal Microvasculature Alterations Affecting the Adult Kidney Function |
title_full |
Imbalance of Pro- and Anti-Angiogenic Factors Due to Maternal Vitamin D Deficiency Causes Renal Microvasculature Alterations Affecting the Adult Kidney Function |
title_fullStr |
Imbalance of Pro- and Anti-Angiogenic Factors Due to Maternal Vitamin D Deficiency Causes Renal Microvasculature Alterations Affecting the Adult Kidney Function |
title_full_unstemmed |
Imbalance of Pro- and Anti-Angiogenic Factors Due to Maternal Vitamin D Deficiency Causes Renal Microvasculature Alterations Affecting the Adult Kidney Function |
title_sort |
imbalance of pro- and anti-angiogenic factors due to maternal vitamin d deficiency causes renal microvasculature alterations affecting the adult kidney function |
publisher |
MDPI AG |
series |
Nutrients |
issn |
2072-6643 |
publishDate |
2019-08-01 |
description |
Vitamin D (Vit.D) is involved in cellular proliferation and differentiation and regulation of the renin gene, which are important aspects of nephrogenesis and quiescence of renal health in adulthood. This study evaluated the angiogenic mechanisms involved in long term renal disturbances induced by Vit.D deficiency persistent in adulthood in rats. First-generation male Hannover offspring from mothers fed either a control diet (control group, CG) or Vit.D-deficient diet (Vit.D- group) were evaluated. Systolic blood pressure (SBP) was measured monthly during the first 6 months after birth, and blood and urine samples were collected to evaluate renal function. Nitric oxide (NO), angiotensin II (ANGII), parathyroid hormone (PTH), calcium, and Vit.D were measured. The kidneys were then removed for morphometric, NO, immunohistochemical, and Western blot studies. We evaluated the expression of vascular growth factor (VEGF) and angiopoietins 1 and 2 and their receptors since this intrinsic renal axis is responsible for endothelial quiescence. Compared to CG, the Vit.D- group presented higher SBP, ANG II plasma levels, renin expression, and AT1 receptor expression levels. Capillary rarefaction was observed, as well as an imbalance between pro- and anti-angiogenic factors. Collectively, the present findings support the role of Vit.D for maintaining the integrity of renal microcirculation. |
topic |
angiogenesis renin-angiotensin system renal microcirculation vitamin D deficiency |
url |
https://www.mdpi.com/2072-6643/11/8/1929 |
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