Modulation of Conductivity of Alginate Hydrogels Containing Reduced Graphene Oxide through the Addition of Proteins
Modifying hydrogels in order to enhance their conductivity is an exciting field with applications in cardio and neuro-regenerative medicine. Therefore, we have designed hybrid alginate hydrogels containing uncoated and protein-coated reduced graphene oxide (rGO). We specifically studied the adsorpti...
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doaj-3c882cbb124e43b58865052ffcce35192021-09-26T00:57:01ZengMDPI AGPharmaceutics1999-49232021-09-01131473147310.3390/pharmaceutics13091473Modulation of Conductivity of Alginate Hydrogels Containing Reduced Graphene Oxide through the Addition of ProteinsAhmed Raslan0Jesús Ciriza1Ana María Ochoa de Retana2María Luisa Sanjuán3Muhammet S. Toprak4Patricia Galvez-Martin5Laura Saenz-del-Burgo6Jose Luis Pedraz7NanoBioCel Group, Laboratory of Pharmacy and Pharmaceutical Technology, Faculty of Pharmacy, University of the Basque Country UPV/EHU, 01006 Vitoria-Gasteiz, SpainBiomedical Research Networking Center in Bioengineering, Biomaterials, and Nanomedicine, CIBER-BBN, Paseo de la Universidad 7, 01006 Vitoria-Gasteiz, SpainDepartment of Organic Chemistry I, Faculty of Pharmacy and Lascaray Research Center, University of the Basque Country (UPV/EHU), Paseo de la Universidad 7, 01006 Vitoria-Gasteiz, SpainInstituto de Ciencia de Materiales de Aragón (Universidad de Zaragoza-CSIC), Facultad de Ciencias, 50009 Zaragoza, SpainBiomedical and X-ray Physics, Department of Applied Physics, KTH-Royal Institute of Technology, 10691 Stockholm, SwedenR&D Animal and Human Health, Bioibérica S.A.U., 08029 Barcelona, SpainNanoBioCel Group, Laboratory of Pharmacy and Pharmaceutical Technology, Faculty of Pharmacy, University of the Basque Country UPV/EHU, 01006 Vitoria-Gasteiz, SpainNanoBioCel Group, Laboratory of Pharmacy and Pharmaceutical Technology, Faculty of Pharmacy, University of the Basque Country UPV/EHU, 01006 Vitoria-Gasteiz, SpainModifying hydrogels in order to enhance their conductivity is an exciting field with applications in cardio and neuro-regenerative medicine. Therefore, we have designed hybrid alginate hydrogels containing uncoated and protein-coated reduced graphene oxide (rGO). We specifically studied the adsorption of three different proteins, BSA, elastin, and collagen, and the outcomes when these protein-coated rGO nanocomposites are embedded within the hydrogels. Our results demonstrate that BSA, elastin, and collagen are adsorbed onto the rGO surface, through a non-spontaneous phenomenon that fits Langmuir and pseudo-second-order adsorption models. Protein-coated rGOs are able to preclude further adsorption of erythropoietin, but not insulin. Collagen showed better adsorption capacity than BSA and elastin due to its hydrophobic nature, although requiring more energy. Moreover, collagen-coated rGO hybrid alginate hydrogels showed an enhancement in conductivity, showing that it could be a promising conductive scaffold for regenerative medicine.https://www.mdpi.com/1999-4923/13/9/1473hydrogelalginatereduced graphene oxideconductivitycollagen |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ahmed Raslan Jesús Ciriza Ana María Ochoa de Retana María Luisa Sanjuán Muhammet S. Toprak Patricia Galvez-Martin Laura Saenz-del-Burgo Jose Luis Pedraz |
spellingShingle |
Ahmed Raslan Jesús Ciriza Ana María Ochoa de Retana María Luisa Sanjuán Muhammet S. Toprak Patricia Galvez-Martin Laura Saenz-del-Burgo Jose Luis Pedraz Modulation of Conductivity of Alginate Hydrogels Containing Reduced Graphene Oxide through the Addition of Proteins Pharmaceutics hydrogel alginate reduced graphene oxide conductivity collagen |
author_facet |
Ahmed Raslan Jesús Ciriza Ana María Ochoa de Retana María Luisa Sanjuán Muhammet S. Toprak Patricia Galvez-Martin Laura Saenz-del-Burgo Jose Luis Pedraz |
author_sort |
Ahmed Raslan |
title |
Modulation of Conductivity of Alginate Hydrogels Containing Reduced Graphene Oxide through the Addition of Proteins |
title_short |
Modulation of Conductivity of Alginate Hydrogels Containing Reduced Graphene Oxide through the Addition of Proteins |
title_full |
Modulation of Conductivity of Alginate Hydrogels Containing Reduced Graphene Oxide through the Addition of Proteins |
title_fullStr |
Modulation of Conductivity of Alginate Hydrogels Containing Reduced Graphene Oxide through the Addition of Proteins |
title_full_unstemmed |
Modulation of Conductivity of Alginate Hydrogels Containing Reduced Graphene Oxide through the Addition of Proteins |
title_sort |
modulation of conductivity of alginate hydrogels containing reduced graphene oxide through the addition of proteins |
publisher |
MDPI AG |
series |
Pharmaceutics |
issn |
1999-4923 |
publishDate |
2021-09-01 |
description |
Modifying hydrogels in order to enhance their conductivity is an exciting field with applications in cardio and neuro-regenerative medicine. Therefore, we have designed hybrid alginate hydrogels containing uncoated and protein-coated reduced graphene oxide (rGO). We specifically studied the adsorption of three different proteins, BSA, elastin, and collagen, and the outcomes when these protein-coated rGO nanocomposites are embedded within the hydrogels. Our results demonstrate that BSA, elastin, and collagen are adsorbed onto the rGO surface, through a non-spontaneous phenomenon that fits Langmuir and pseudo-second-order adsorption models. Protein-coated rGOs are able to preclude further adsorption of erythropoietin, but not insulin. Collagen showed better adsorption capacity than BSA and elastin due to its hydrophobic nature, although requiring more energy. Moreover, collagen-coated rGO hybrid alginate hydrogels showed an enhancement in conductivity, showing that it could be a promising conductive scaffold for regenerative medicine. |
topic |
hydrogel alginate reduced graphene oxide conductivity collagen |
url |
https://www.mdpi.com/1999-4923/13/9/1473 |
work_keys_str_mv |
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