Back to Nature: Combating <i>Candida</i> <i>albicans</i> Biofilm, Phospholipase and Hemolysin Using Plant Essential Oils
<i>Candida albicans </i>is the causative agent of fatal systemic candidiasis. Due to limitations of antifungals, new drugs are needed. The anti-virulence effect of plant essential oils (EOs) was evaluated against clinical<i> C. albicans </i>isolates including cinnamon, clove,...
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doaj-3c91b7434bd04c858b6bede30870973c2021-01-16T00:06:06ZengMDPI AGAntibiotics2079-63822021-01-0110818110.3390/antibiotics10010081Back to Nature: Combating <i>Candida</i> <i>albicans</i> Biofilm, Phospholipase and Hemolysin Using Plant Essential OilsAhmed M. El-Baz0Rasha A. Mosbah1Reham M. Goda2Basem Mansour3Taranum Sultana4Tanya E. S. Dahms5Amira M. El-Ganiny6Microbiology and Biotechnology Department, Faculty of Pharmacy, Delta University for Science and Technology, International Coastal Road, Gamasa City, Mansoura, 11152 Dakhaliya, EgyptInfection Control Unit, Zagazig University hospitals, 44519 Zagazig, EgyptMicrobiology and Biotechnology Department, Faculty of Pharmacy, Delta University for Science and Technology, International Coastal Road, Gamasa City, Mansoura, 11152 Dakhaliya, EgyptPharmaceutical Chemistry Department, Faculty of Pharmacy, Delta University for Science and Technology, International Coastal Road, Gamasa City, Mansoura, 11152 Dakhaliya, EgyptDepartment of Chemistry and Biochemistry, University of Regina, Regina, SK S4S 1P4, CanadaDepartment of Chemistry and Biochemistry, University of Regina, Regina, SK S4S 1P4, CanadaMicrobiology and Immunology Department, Faculty of Pharmacy, Zagazig University, 44519 Zagazig, Egypt<i>Candida albicans </i>is the causative agent of fatal systemic candidiasis. Due to limitations of antifungals, new drugs are needed. The anti-virulence effect of plant essential oils (EOs) was evaluated against clinical<i> C. albicans </i>isolates including cinnamon, clove, jasmine and rosemary oils. Biofilm, phospholipase and hemolysin were assessed phenotypically. EOs were evaluated for their anti-virulence activity using phenotypic methods as well as scanning electron microscopy (SEM) and atomic force microscopy (AFM). Among the <i>C. albicans</i> isolates, biofilm, phospholipase and hemolysins were detected in 40.4, 86.5 and 78.8% of isolates, respectively. Jasmine oil showed the highest anti-biofilm activity followed by cinnamon, clove and rosemary oils. SEM and AFM analysis showed reduced adherence and roughness in the presence of EOs. For phospholipase, rosemary oil was the most inhibitory, followed by jasmine, cinnamon and clove oils, and for hemolysins, cinnamon had the highest inhibition followed by jasmine, rosemary and clove oils. A molecular docking study revealed major EO constituents as promising inhibitors of the Als3 adhesive protein, with the highest binding for eugenol, followed by 1,8-cineole, 2-phenylthiolane and cinnamaldehyde. In conclusion, EOs have a promising inhibitory impact on Candida biofilm, phospholipase and hemolysin production, hence EOs could be used as potential antifungals that impact virulence factors.https://www.mdpi.com/2079-6382/10/1/81<i>Candida albicans</i>essential oilsjasmine oilbiofilmSEMAFM |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ahmed M. El-Baz Rasha A. Mosbah Reham M. Goda Basem Mansour Taranum Sultana Tanya E. S. Dahms Amira M. El-Ganiny |
spellingShingle |
Ahmed M. El-Baz Rasha A. Mosbah Reham M. Goda Basem Mansour Taranum Sultana Tanya E. S. Dahms Amira M. El-Ganiny Back to Nature: Combating <i>Candida</i> <i>albicans</i> Biofilm, Phospholipase and Hemolysin Using Plant Essential Oils Antibiotics <i>Candida albicans</i> essential oils jasmine oil biofilm SEM AFM |
author_facet |
Ahmed M. El-Baz Rasha A. Mosbah Reham M. Goda Basem Mansour Taranum Sultana Tanya E. S. Dahms Amira M. El-Ganiny |
author_sort |
Ahmed M. El-Baz |
title |
Back to Nature: Combating <i>Candida</i> <i>albicans</i> Biofilm, Phospholipase and Hemolysin Using Plant Essential Oils |
title_short |
Back to Nature: Combating <i>Candida</i> <i>albicans</i> Biofilm, Phospholipase and Hemolysin Using Plant Essential Oils |
title_full |
Back to Nature: Combating <i>Candida</i> <i>albicans</i> Biofilm, Phospholipase and Hemolysin Using Plant Essential Oils |
title_fullStr |
Back to Nature: Combating <i>Candida</i> <i>albicans</i> Biofilm, Phospholipase and Hemolysin Using Plant Essential Oils |
title_full_unstemmed |
Back to Nature: Combating <i>Candida</i> <i>albicans</i> Biofilm, Phospholipase and Hemolysin Using Plant Essential Oils |
title_sort |
back to nature: combating <i>candida</i> <i>albicans</i> biofilm, phospholipase and hemolysin using plant essential oils |
publisher |
MDPI AG |
series |
Antibiotics |
issn |
2079-6382 |
publishDate |
2021-01-01 |
description |
<i>Candida albicans </i>is the causative agent of fatal systemic candidiasis. Due to limitations of antifungals, new drugs are needed. The anti-virulence effect of plant essential oils (EOs) was evaluated against clinical<i> C. albicans </i>isolates including cinnamon, clove, jasmine and rosemary oils. Biofilm, phospholipase and hemolysin were assessed phenotypically. EOs were evaluated for their anti-virulence activity using phenotypic methods as well as scanning electron microscopy (SEM) and atomic force microscopy (AFM). Among the <i>C. albicans</i> isolates, biofilm, phospholipase and hemolysins were detected in 40.4, 86.5 and 78.8% of isolates, respectively. Jasmine oil showed the highest anti-biofilm activity followed by cinnamon, clove and rosemary oils. SEM and AFM analysis showed reduced adherence and roughness in the presence of EOs. For phospholipase, rosemary oil was the most inhibitory, followed by jasmine, cinnamon and clove oils, and for hemolysins, cinnamon had the highest inhibition followed by jasmine, rosemary and clove oils. A molecular docking study revealed major EO constituents as promising inhibitors of the Als3 adhesive protein, with the highest binding for eugenol, followed by 1,8-cineole, 2-phenylthiolane and cinnamaldehyde. In conclusion, EOs have a promising inhibitory impact on Candida biofilm, phospholipase and hemolysin production, hence EOs could be used as potential antifungals that impact virulence factors. |
topic |
<i>Candida albicans</i> essential oils jasmine oil biofilm SEM AFM |
url |
https://www.mdpi.com/2079-6382/10/1/81 |
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