Evaluation of buccal damage associated with acute inhalation exposure to 2,4-dichlorophenoxyacetic acid (2,4-D) in mice

• Main Authors: José Luiz Santos Parizi, Amanda Jodas Tolardo, Ana Carolina Gomes Lisboa, Bruna Barravieira, Fabíola de Azevedo Mello, Renata Calciolari Rossi, Gisele Alborghetti Nai
• Format: Article
• Language: English
• Published: BMC 2020-07-01
• Series: BMC Veterinary Research
• Subjects: Leukoplakia; oral; Inflammation; Mast cells; Pesticide exposure; Environmental exposure
• Online Access: http://link.springer.com/article/10.1186/s12917-020-02461-w

Abstract Background The herbicide dichlorophenoxyacetic acid (2,4-D) is one of the most widely used crop spraying products in the world. Some pesticides induce the degranulation of mast cells and increase allergic responses. This is the first study to evaluate the damage to the oral mucosa after an experimental simulation of environmental inhalation exposure to the 2,4-D herbicide. The aim of this study was evaluate the possible oral damage caused by acute inhalation exposure to the herbicide 2,4-D. Results There was a difference between the exposure concentrations in relation to tissue congestion intensity (p = 0.002) and mast cell counts (p = 0.002), a difference in the evaluation of the interaction between the exposure concentrations and nebulization time in the dorsum epithelium thickness (p = 0.013), and a significant correlation between the epithelial thickness and the number of nucleoli organizing regions on the dorsum of the tongue (p = 0.048). Conclusions Even after acute exposure, the herbicide 2,4-D had the potential to damage the oral epithelium, especially at higher doses.