Antimicrobial resistance, carbapenemase production, and genotypes of nosocomial Acinetobacter spp. isolates in Russia: results of multicenter epidemiological study ”MARATHON 2015–2016”
Objective. Objectives. To assess the rates of antibiotic resistance and production of acquired carbapenemases in nosocomial strains of Acinetobacter spp., and to determine the genotypes and prevalence of “international high-risk clones” among nosocomial strains of Acinetobacter baumannii isolated i...
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Format: | Article |
Language: | Russian |
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Interregional Association for Clinical Microbiology and Antimicrobial Chemotherapy
2019-10-01
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Series: | Клиническая микробиология и антимикробная химиотерапия |
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Online Access: | https://cmac-journal.ru/publication/2019/2/cmac-2019-t21-n2-p171/cmac-2019-t21-n2-p171.pdf |
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author |
Shek E.A. Sukhorukova M.V. Edelstein M.V. Skleenova E.Yu. Ivanchik N.V. Shajdullina E.R. Kuzmenkov A.Yu. Dekhnich A.V. Kozlov R.S. Semyonova N.V. Slepakova S.A. Shepotajlova N.V. Strebkova V.V. Rybina N.A. Yaranceva N.Z. Perevalova E.Yu. Rozanova S.M. Nagovicina S.G. Moldovanu M.G. Nasybullova Z.Z. Arkhipenko M.V. Shakhmuradyan R.M. Nizhegorodceva I.A. Varibrus E.V. Aleksandrova I.A. Lazareva A.V. Kryzhanovskaya O.A. Markelova N.N. Chernyavskaya Yu.L. Lebedeva E.V. Kirillova G.Sh. Bekker G.G. Popova L.D. Elokhina E.V. Smol’kova Yu.E. Zinov’ev D.Yu. Ityaeva L.N. Blinova G.Yu. Zubareva N.A. Vityazeva V.P. Plaksina M.G. Kucevalova O.Yu. Panova N.I. Suborova T.N. Polukhina O.V. Voroshilova T.M. Churikova E.M. Moskvitina E.N. Krechikova O.I. Petrova T.A. Mart’yanova N.M. Khokhlova K.O. Gudkova L.V. Bykonya S.A. Khokhlyavina R.M. Shpil’kina L.V. Burasova E.G. Khrebtovskaya V.A. Molchanova I.V. Zvonaryova O.V. Kornilova P.A. Kryanga V.G. Portnyagina U.S. Shamaeva S.Kh. Popov D.A. Vostrikova T.Yu. |
spellingShingle |
Shek E.A. Sukhorukova M.V. Edelstein M.V. Skleenova E.Yu. Ivanchik N.V. Shajdullina E.R. Kuzmenkov A.Yu. Dekhnich A.V. Kozlov R.S. Semyonova N.V. Slepakova S.A. Shepotajlova N.V. Strebkova V.V. Rybina N.A. Yaranceva N.Z. Perevalova E.Yu. Rozanova S.M. Nagovicina S.G. Moldovanu M.G. Nasybullova Z.Z. Arkhipenko M.V. Shakhmuradyan R.M. Nizhegorodceva I.A. Varibrus E.V. Aleksandrova I.A. Lazareva A.V. Kryzhanovskaya O.A. Markelova N.N. Chernyavskaya Yu.L. Lebedeva E.V. Kirillova G.Sh. Bekker G.G. Popova L.D. Elokhina E.V. Smol’kova Yu.E. Zinov’ev D.Yu. Ityaeva L.N. Blinova G.Yu. Zubareva N.A. Vityazeva V.P. Plaksina M.G. Kucevalova O.Yu. Panova N.I. Suborova T.N. Polukhina O.V. Voroshilova T.M. Churikova E.M. Moskvitina E.N. Krechikova O.I. Petrova T.A. Mart’yanova N.M. Khokhlova K.O. Gudkova L.V. Bykonya S.A. Khokhlyavina R.M. Shpil’kina L.V. Burasova E.G. Khrebtovskaya V.A. Molchanova I.V. Zvonaryova O.V. Kornilova P.A. Kryanga V.G. Portnyagina U.S. Shamaeva S.Kh. Popov D.A. Vostrikova T.Yu. Antimicrobial resistance, carbapenemase production, and genotypes of nosocomial Acinetobacter spp. isolates in Russia: results of multicenter epidemiological study ”MARATHON 2015–2016” Клиническая микробиология и антимикробная химиотерапия nosocomial infections acinetobacter spp a. baumannii antimicrobial resistance carbapenemases |
author_facet |
Shek E.A. Sukhorukova M.V. Edelstein M.V. Skleenova E.Yu. Ivanchik N.V. Shajdullina E.R. Kuzmenkov A.Yu. Dekhnich A.V. Kozlov R.S. Semyonova N.V. Slepakova S.A. Shepotajlova N.V. Strebkova V.V. Rybina N.A. Yaranceva N.Z. Perevalova E.Yu. Rozanova S.M. Nagovicina S.G. Moldovanu M.G. Nasybullova Z.Z. Arkhipenko M.V. Shakhmuradyan R.M. Nizhegorodceva I.A. Varibrus E.V. Aleksandrova I.A. Lazareva A.V. Kryzhanovskaya O.A. Markelova N.N. Chernyavskaya Yu.L. Lebedeva E.V. Kirillova G.Sh. Bekker G.G. Popova L.D. Elokhina E.V. Smol’kova Yu.E. Zinov’ev D.Yu. Ityaeva L.N. Blinova G.Yu. Zubareva N.A. Vityazeva V.P. Plaksina M.G. Kucevalova O.Yu. Panova N.I. Suborova T.N. Polukhina O.V. Voroshilova T.M. Churikova E.M. Moskvitina E.N. Krechikova O.I. Petrova T.A. Mart’yanova N.M. Khokhlova K.O. Gudkova L.V. Bykonya S.A. Khokhlyavina R.M. Shpil’kina L.V. Burasova E.G. Khrebtovskaya V.A. Molchanova I.V. Zvonaryova O.V. Kornilova P.A. Kryanga V.G. Portnyagina U.S. Shamaeva S.Kh. Popov D.A. Vostrikova T.Yu. |
author_sort |
Shek E.A. |
title |
Antimicrobial resistance, carbapenemase production, and genotypes of nosocomial Acinetobacter spp. isolates in Russia: results of multicenter epidemiological study ”MARATHON 2015–2016” |
title_short |
Antimicrobial resistance, carbapenemase production, and genotypes of nosocomial Acinetobacter spp. isolates in Russia: results of multicenter epidemiological study ”MARATHON 2015–2016” |
title_full |
Antimicrobial resistance, carbapenemase production, and genotypes of nosocomial Acinetobacter spp. isolates in Russia: results of multicenter epidemiological study ”MARATHON 2015–2016” |
title_fullStr |
Antimicrobial resistance, carbapenemase production, and genotypes of nosocomial Acinetobacter spp. isolates in Russia: results of multicenter epidemiological study ”MARATHON 2015–2016” |
title_full_unstemmed |
Antimicrobial resistance, carbapenemase production, and genotypes of nosocomial Acinetobacter spp. isolates in Russia: results of multicenter epidemiological study ”MARATHON 2015–2016” |
title_sort |
antimicrobial resistance, carbapenemase production, and genotypes of nosocomial acinetobacter spp. isolates in russia: results of multicenter epidemiological study ”marathon 2015–2016” |
publisher |
Interregional Association for Clinical Microbiology and Antimicrobial Chemotherapy |
series |
Клиническая микробиология и антимикробная химиотерапия |
issn |
1684-4386 2686-9586 |
publishDate |
2019-10-01 |
description |
Objective.
Objectives. To assess the rates of antibiotic resistance and production of acquired carbapenemases in nosocomial strains of Acinetobacter spp., and to determine the genotypes and prevalence of “international high-risk clones” among nosocomial strains of Acinetobacter baumannii isolated in various regions of Russia within the “MARATHON 2015–2016” study.
Materials and Methods.
A total of 1005 non-duplicate nosocomial isolates of Acinetobacter spp., including 975 isolates of A. baumannii, collected in 44 hospitals from 25 cities in Russia in 2015– 2016 were studied. Species identification of isolates was performed by means of MALDI-TOF massspectrometry. Antimicrobial susceptibility was determined using broth microdilution method according to ISO 20776-1:2006 and interpreted using EUCAST MIC clinical breakpoints v.9.0. The presence of acquired carbapenemase genes of OXA-23, OXA-24/40, OXA-58, VIM, IMP, NDM, GES-2 and GES-5 groups was determined using real-time PCR. Genotyping of A. baumannii isolates was performed by analysis of selected single nucleotide polymorphisms in 10 chromosomal loci used for multi-locus sequence tying (MLST) of this species.
Results.
Acinetobacter spp. and A. baumannii comprised of 17.4% and 16.8% of all bacterial pathogens isolated within the “MARATHON 2015–2016” study. Resistance rates to carbapenems (imipenem and meropenem) were 77.4% and 77.1% of A. baumannii isolates, respectively. In 76.2% of A. baumannii isolates, the genes of acquired carbapenemases of molecular class D were detected, which belonged to the following groups: OXA-24/40 (57.5%), OXA-23 (18.4%) and OXA-58 (0.1%). Two isolates (0.2%) carried the genes of OXA-24/40- and OXA-23-like carbapenemases simultaneously. Most carbapenemaseproducing isolates belonged to the «international high-risk clones»: CC92/208OXF/CC2PAS (60.3%), CC944OXF/CC78PAS (25.4%) and CC109/231OXF/CC1PAS (11.6%). The vast majority of A. baumannii isolates exhibited resistance to ciprofloxacin (99.0%), amikacin (89.2%) and gentamicin (77.4%). The prevalence of resistance to tobramycin and trimethoprim/sulfamethoxazole was variable among strains of different genotypes. Colistin was the most active agent in vitro (0.9% of resistant isolates). Isolates of other Acinetobacter species were more susceptible to most antibiotics. However, two isolates of Acinetobacter ursingii and Acinetobacter baylyi carried the genes of NDM metallo-beta-lactamases.
Conclusions.
The results of this study indicate a very high prevalence of resistance to most antibiotics, including carbapenems, in nosocomial strains of Acinetobacter spp. This makes the selection of empiric antibiotic treatment extremely difficult and urges the need for regular local surveillance of resistance in every hospital. |
topic |
nosocomial infections acinetobacter spp a. baumannii antimicrobial resistance carbapenemases |
url |
https://cmac-journal.ru/publication/2019/2/cmac-2019-t21-n2-p171/cmac-2019-t21-n2-p171.pdf |
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doaj-3cb8928085e14ec398a42d228484f3722020-11-25T01:35:59ZrusInterregional Association for Clinical Microbiology and Antimicrobial ChemotherapyКлиническая микробиология и антимикробная химиотерапия1684-43862686-95862019-10-0121217118010.36488/cmac.2019.2.171-180cmac-2019-t21-n2-p171Antimicrobial resistance, carbapenemase production, and genotypes of nosocomial Acinetobacter spp. isolates in Russia: results of multicenter epidemiological study ”MARATHON 2015–2016”Shek E.A.0Sukhorukova M.V.1Edelstein M.V.2Skleenova E.Yu.3Ivanchik N.V.4Shajdullina E.R.5Kuzmenkov A.Yu.6Dekhnich A.V.7Kozlov R.S.8Semyonova N.V.9Slepakova S.A.10Shepotajlova N.V.11Strebkova V.V.12Rybina N.A.13Yaranceva N.Z.14Perevalova E.Yu.15Rozanova S.M.16Nagovicina S.G.17Moldovanu M.G.18Nasybullova Z.Z.19Arkhipenko M.V.20Shakhmuradyan R.M.21Nizhegorodceva I.A.22Varibrus E.V.23Aleksandrova I.A.24Lazareva A.V.25Kryzhanovskaya O.A.26Markelova N.N.27Chernyavskaya Yu.L.28Lebedeva E.V.29Kirillova G.Sh.30Bekker G.G.31Popova L.D.32Elokhina E.V.33Smol’kova Yu.E.34Zinov’ev D.Yu.35Ityaeva L.N.36Blinova G.Yu.37Zubareva N.A.38Vityazeva V.P.39Plaksina M.G.40Kucevalova O.Yu.41Panova N.I.42Suborova T.N.43Polukhina O.V.44Voroshilova T.M.45Churikova E.M.46Moskvitina E.N.47Krechikova O.I.48Petrova T.A.49Mart’yanova N.M.50Khokhlova K.O.51Gudkova L.V.52Bykonya S.A.53Khokhlyavina R.M.54Shpil’kina L.V.55Burasova E.G.56Khrebtovskaya V.A.57Molchanova I.V.58Zvonaryova O.V.59Kornilova P.A.60Kryanga V.G.61Portnyagina U.S.62Shamaeva S.Kh.63Popov D.A.64Vostrikova T.Yu.65Institute of Antimicrobial Chemotherapy, Smolensk, RussiaInstitute of Antimicrobial Chemotherapy, Smolensk, RussiaInstitute of Antimicrobial Chemotherapy, Smolensk, RussiaInstitute of Antimicrobial Chemotherapy, Smolensk, RussiaInstitute of Antimicrobial Chemotherapy, Smolensk, RussiaInstitute of Antimicrobial Chemotherapy, Smolensk, RussiaInstitute of Antimicrobial Chemotherapy, Smolensk, RussiaInstitute of Antimicrobial Chemotherapy, Smolensk, RussiaInstitute of Antimicrobial Chemotherapy, Smolensk, RussiaArkhangelsk Regional Clinical Hospital, Arkhangelsk, RussiaAmur Regional Children Clinical Hospital, Blagoveshchensk, RussiaAmur Regional Children Clinical Hospital, Blagoveshchensk, RussiaVoronezh City Clinical Emergency Care HospitalVoronezh City Clinical Emergency Care HospitalSverdlovsk Regional Clinical Psychiatric Hospital, Yekaterinburg, RussiaClinical Diagnostic Center, Yekaterinburg, RussiaClinical Diagnostic Center, Yekaterinburg, RussiaCity Clinical HospitalCity Clinical HospitalRepublican Clinical Hospital, Kazan, RussiaResearch Institute – Regional Clinical HospitalResearch Institute – Regional Clinical HospitalRegional Clinical HospitalRegional Clinical HospitalNational Medical Research Center of Neurosurgery named after N.N. Burdenko, Moscow, RussiaNational Medical Research Center for Children’s Health, Moscow, RussiaNational Medical Research Center for Children’s Health, Moscow, RussiaRussian Scientific Center of Roentgenoradiology, Moscow, RussiaMurmansk Regional Clinical Hospital named after P.A. Bayandin, Murmansk, RussiaMurmansk Regional Clinical Hospital named after P.A. Bayandin, Murmansk, RussiaEmergency Care Hospital, Naberezhnye Chelny, RussiaClinical Hospital at the Novosibirsk-Main Station, Novosibirsk, RussiaRegional Clinical Hospital, Omsk, RussiaRegional Clinical Hospital, Omsk, RussiaClinical HospitalClinical HospitalRegional Oncology Dispensary, Penza, RussiaRegional Oncology Dispensary, Penza, RussiaPerm State Medical University named after E.A. Wagner, Perm, RussiaChildren Republican Hospital, Petrozavodsk, RussiaChildren Republican Hospital, Petrozavodsk, RussiaRostov Research Institute of Oncology, Rostov-on-Don, RussiaRostov Research Institute of Oncology, Rostov-on-Don, RussiaS.M. Kirov Military Medical Academy, Saint-Petersburg, RussiaSaint-Petersburg, RussiaAll-Russian Center of Emergency and Radiation Medicine named after A.M. Nikiforov, Saint-Petersburg, RussiaAll-Russian Center of Emergency and Radiation Medicine named after A.M. Nikiforov, Saint-Petersburg, RussiaSiberian Federal Scientific Clinical Center, Seversk, RussiaInstitute of Antimicrobial Chemotherapy, Smolensk, RussiaClinical HospitalTol’yatti City Clinical HospitalTol’yatti City Clinical HospitalTomsk Regional Clinical Hospital, Tomsk, RussiaTomsk Regional Clinical Hospital, Tomsk, RussiaRegional Clinical HospitalRegional Clinical HospitalRepublican Clinical Hospital named after N.A. Semashko, Ulan-Ude, RussiaRepublican Clinical Hospital named after N.A. Semashko, Ulan-Ude, RussiaChelyabinsk Regional Clinical Hospital, Chelyabinsk, RussiaChelyabinsk Regional Clinical Hospital, Chelyabinsk, RussiaRepublican HospitalRepublican HospitalRepublican HospitalRepublican HospitalA.N. Bakulev National Medical Research Center of Cardiovascular Surgery, Moscow, RussiaA.N. Bakulev National Medical Research Center of Cardiovascular Surgery, Moscow, RussiaObjective. Objectives. To assess the rates of antibiotic resistance and production of acquired carbapenemases in nosocomial strains of Acinetobacter spp., and to determine the genotypes and prevalence of “international high-risk clones” among nosocomial strains of Acinetobacter baumannii isolated in various regions of Russia within the “MARATHON 2015–2016” study. Materials and Methods. A total of 1005 non-duplicate nosocomial isolates of Acinetobacter spp., including 975 isolates of A. baumannii, collected in 44 hospitals from 25 cities in Russia in 2015– 2016 were studied. Species identification of isolates was performed by means of MALDI-TOF massspectrometry. Antimicrobial susceptibility was determined using broth microdilution method according to ISO 20776-1:2006 and interpreted using EUCAST MIC clinical breakpoints v.9.0. The presence of acquired carbapenemase genes of OXA-23, OXA-24/40, OXA-58, VIM, IMP, NDM, GES-2 and GES-5 groups was determined using real-time PCR. Genotyping of A. baumannii isolates was performed by analysis of selected single nucleotide polymorphisms in 10 chromosomal loci used for multi-locus sequence tying (MLST) of this species. Results. Acinetobacter spp. and A. baumannii comprised of 17.4% and 16.8% of all bacterial pathogens isolated within the “MARATHON 2015–2016” study. Resistance rates to carbapenems (imipenem and meropenem) were 77.4% and 77.1% of A. baumannii isolates, respectively. In 76.2% of A. baumannii isolates, the genes of acquired carbapenemases of molecular class D were detected, which belonged to the following groups: OXA-24/40 (57.5%), OXA-23 (18.4%) and OXA-58 (0.1%). Two isolates (0.2%) carried the genes of OXA-24/40- and OXA-23-like carbapenemases simultaneously. Most carbapenemaseproducing isolates belonged to the «international high-risk clones»: CC92/208OXF/CC2PAS (60.3%), CC944OXF/CC78PAS (25.4%) and CC109/231OXF/CC1PAS (11.6%). The vast majority of A. baumannii isolates exhibited resistance to ciprofloxacin (99.0%), amikacin (89.2%) and gentamicin (77.4%). The prevalence of resistance to tobramycin and trimethoprim/sulfamethoxazole was variable among strains of different genotypes. Colistin was the most active agent in vitro (0.9% of resistant isolates). Isolates of other Acinetobacter species were more susceptible to most antibiotics. However, two isolates of Acinetobacter ursingii and Acinetobacter baylyi carried the genes of NDM metallo-beta-lactamases. Conclusions. The results of this study indicate a very high prevalence of resistance to most antibiotics, including carbapenems, in nosocomial strains of Acinetobacter spp. This makes the selection of empiric antibiotic treatment extremely difficult and urges the need for regular local surveillance of resistance in every hospital.https://cmac-journal.ru/publication/2019/2/cmac-2019-t21-n2-p171/cmac-2019-t21-n2-p171.pdfnosocomial infectionsacinetobacter sppa. baumanniiantimicrobial resistancecarbapenemases |