Recombinant soluble form of the high-affinity IgE receptor α subunit and anti-IgE antibody inhibit IgE synthesis by IgE-expressing B cells through distinct pathways
Background: Both a recombinant soluble form of the high-affinity IgE receptor α subunit (rsFcεRIα) and anti-IgE antibody have been shown to be involved in the regulation of IgE synthesis. However, the mechanisms of IgE regulation by two such IgE-binding agents remain unclear. In the present study, w...
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doaj-3cd2b1a8d06f459093f9fe24db5982c82020-11-24T22:01:19ZengElsevierAllergology International1323-89302002-01-0151317518410.1046/j.1440-1592.2002.00262.xRecombinant soluble form of the high-affinity IgE receptor α subunit and anti-IgE antibody inhibit IgE synthesis by IgE-expressing B cells through distinct pathwaysKeiichi Kajiwara0Chisei Ra1Yukiyoshi Yanagihara2Clinical Research Center, National Sagamihara Hospital, SagamiharaAdvanced Medical Research Center, Nihon University School of Medicine, Tokyo, JapanClinical Research Center, National Sagamihara Hospital, SagamiharaBackground: Both a recombinant soluble form of the high-affinity IgE receptor α subunit (rsFcεRIα) and anti-IgE antibody have been shown to be involved in the regulation of IgE synthesis. However, the mechanisms of IgE regulation by two such IgE-binding agents remain unclear. In the present study, we investigated whether rsFcεRIα and anti-IgE antibody modulated IgE synthesis in an identical or different manner. Methods: Normal human B cells stimulated with interleukin (IL)-4 plus anti-CD40 antibody were analyzed for the regulatory effects of rsFcεRIα and anti-IgE antibody on the expression of Cε transcipts, the autocrine production of IL-6 and the induction of apoptosis. Results: Both rsFcεRIα and anti-IgE antibody inhibited mature Cε transcription, without affecting germline Cε transcription. In addition, rsFcεRIα was effective in decreasing IL-6 production at a later stage when IgE-expressing B cells were generated, whereas F(ab′)2, but not the Fab fragment, of anti-IgE antibody induced apoptosis in the cells. Although these three agents almost equally recognized IgE expressed on B cells, rsFcεRIα was unable to induce apoptotic cell death and the Fab fragment was similarly ineffective in the regulation of IL-6 production. The addition of IL-6 to cultures containing rsFcεRIα significantly restored its suppressive effect on IgE synthesis. Conclusions: These results indicate that regulation of IgE synthesis by rsFcεRIα differs from that by anti-IgE antibody.http://www.sciencedirect.com/science/article/pii/S1323893015313307apoptosisdivalent recognitionIgE-binding agentsIgE-expressing B cellsinterleukin-6monovalent recognition |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Keiichi Kajiwara Chisei Ra Yukiyoshi Yanagihara |
spellingShingle |
Keiichi Kajiwara Chisei Ra Yukiyoshi Yanagihara Recombinant soluble form of the high-affinity IgE receptor α subunit and anti-IgE antibody inhibit IgE synthesis by IgE-expressing B cells through distinct pathways Allergology International apoptosis divalent recognition IgE-binding agents IgE-expressing B cells interleukin-6 monovalent recognition |
author_facet |
Keiichi Kajiwara Chisei Ra Yukiyoshi Yanagihara |
author_sort |
Keiichi Kajiwara |
title |
Recombinant soluble form of the high-affinity IgE receptor α subunit and anti-IgE antibody inhibit IgE synthesis by IgE-expressing B cells through distinct pathways |
title_short |
Recombinant soluble form of the high-affinity IgE receptor α subunit and anti-IgE antibody inhibit IgE synthesis by IgE-expressing B cells through distinct pathways |
title_full |
Recombinant soluble form of the high-affinity IgE receptor α subunit and anti-IgE antibody inhibit IgE synthesis by IgE-expressing B cells through distinct pathways |
title_fullStr |
Recombinant soluble form of the high-affinity IgE receptor α subunit and anti-IgE antibody inhibit IgE synthesis by IgE-expressing B cells through distinct pathways |
title_full_unstemmed |
Recombinant soluble form of the high-affinity IgE receptor α subunit and anti-IgE antibody inhibit IgE synthesis by IgE-expressing B cells through distinct pathways |
title_sort |
recombinant soluble form of the high-affinity ige receptor α subunit and anti-ige antibody inhibit ige synthesis by ige-expressing b cells through distinct pathways |
publisher |
Elsevier |
series |
Allergology International |
issn |
1323-8930 |
publishDate |
2002-01-01 |
description |
Background: Both a recombinant soluble form of the high-affinity IgE receptor α subunit (rsFcεRIα) and anti-IgE antibody have been shown to be involved in the regulation of IgE synthesis. However, the mechanisms of IgE regulation by two such IgE-binding agents remain unclear. In the present study, we investigated whether rsFcεRIα and anti-IgE antibody modulated IgE synthesis in an identical or different manner.
Methods: Normal human B cells stimulated with interleukin (IL)-4 plus anti-CD40 antibody were analyzed for the regulatory effects of rsFcεRIα and anti-IgE antibody on the expression of Cε transcipts, the autocrine production of IL-6 and the induction of apoptosis.
Results: Both rsFcεRIα and anti-IgE antibody inhibited mature Cε transcription, without affecting germline Cε transcription. In addition, rsFcεRIα was effective in decreasing IL-6 production at a later stage when IgE-expressing B cells were generated, whereas F(ab′)2, but not the Fab fragment, of anti-IgE antibody induced apoptosis in the cells. Although these three agents almost equally recognized IgE expressed on B cells, rsFcεRIα was unable to induce apoptotic cell death and the Fab fragment was similarly ineffective in the regulation of IL-6 production. The addition of IL-6 to cultures containing rsFcεRIα significantly restored its suppressive effect on IgE synthesis.
Conclusions: These results indicate that regulation of IgE synthesis by rsFcεRIα differs from that by anti-IgE antibody. |
topic |
apoptosis divalent recognition IgE-binding agents IgE-expressing B cells interleukin-6 monovalent recognition |
url |
http://www.sciencedirect.com/science/article/pii/S1323893015313307 |
work_keys_str_mv |
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