Forensic Application of Monoclonal Anti-Human Glycophorin A Antibody in Samples from Decomposed Bodies to Establish Vitality of the Injuries. A Preliminary Experimental Study
Glycophorins are an important group of red blood cell (RBC) transmembrane proteins. Monoclonal antibodies against GPA are employed in immunohistochemical staining during post-mortem examination: Through this method, it is possible to point out the RBC presence in tissues. This experimental study aim...
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doaj-3cfb612cc8624fa08475fca67fc784ff2021-04-29T23:00:06ZengMDPI AGHealthcare2227-90322021-04-01951451410.3390/healthcare9050514Forensic Application of Monoclonal Anti-Human Glycophorin A Antibody in Samples from Decomposed Bodies to Establish Vitality of the Injuries. A Preliminary Experimental StudyBenedetta Baldari0Simona Vittorio1Francesco Sessa2Luigi Cipolloni3Giuseppe Bertozzi4Margherita Neri5Santina Cantatore6Vittorio Fineschi7Mariarosaria Aromatario8Department of Anatomical, Histological, Forensic and Orthopedic Sciences, Sapienza University of Rome, 00161 Rome, ItalyDepartment of Anatomical, Histological, Forensic and Orthopedic Sciences, Sapienza University of Rome, 00161 Rome, ItalySection of Legal Medicine, Department of Clinical and Experimental Medicine, University of Foggia, 71122 Foggia, ItalySection of Legal Medicine, Department of Clinical and Experimental Medicine, University of Foggia, 71122 Foggia, ItalySection of Legal Medicine, Department of Clinical and Experimental Medicine, University of Foggia, 71122 Foggia, ItalyDepartment of Medical Sciences, University of Ferrara, 44121 Ferrara, ItalySection of Legal Medicine, Department of Clinical and Experimental Medicine, University of Foggia, 71122 Foggia, ItalyDepartment of Anatomical, Histological, Forensic and Orthopedic Sciences, Sapienza University of Rome, 00161 Rome, ItalyLegal Medicine Division, Sant’Andrea University Hospital, 00189 Rome, ItalyGlycophorins are an important group of red blood cell (RBC) transmembrane proteins. Monoclonal antibodies against GPA are employed in immunohistochemical staining during post-mortem examination: Through this method, it is possible to point out the RBC presence in tissues. This experimental study aims to investigate anti-GPA immunohistochemical staining in order to evaluate the vitality of the lesion from corpses in different decomposition state. Six cases were selected, analyzing autopsies’ documentation performed by the Institute of Legal Medicine of Rome in 2010–2018: four samples of fractured bones and three samples of soft tissues. For the control case, the fracture region of the femur was sampled. The results of the present study confirm the preliminary results of other studies, remarking the importance of the GPA immunohistochemical staining to highlight signs of survival. Moreover, this study suggests that the use of this technique should be routinely applied in cases of corpses with advanced putrefaction phenomena, even when the radiological investigation is performed, the macroscopic investigation is inconclusive, the H&E staining is not reliable. This experimental application demonstrated that the use of monoclonal antibody anti-human GPA on bone fractures and soft tissues could be important to verify whether the lesion is vital or not.https://www.mdpi.com/2227-9032/9/5/514forensic pathologyglycophorin A investigationvitality |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Benedetta Baldari Simona Vittorio Francesco Sessa Luigi Cipolloni Giuseppe Bertozzi Margherita Neri Santina Cantatore Vittorio Fineschi Mariarosaria Aromatario |
spellingShingle |
Benedetta Baldari Simona Vittorio Francesco Sessa Luigi Cipolloni Giuseppe Bertozzi Margherita Neri Santina Cantatore Vittorio Fineschi Mariarosaria Aromatario Forensic Application of Monoclonal Anti-Human Glycophorin A Antibody in Samples from Decomposed Bodies to Establish Vitality of the Injuries. A Preliminary Experimental Study Healthcare forensic pathology glycophorin A investigation vitality |
author_facet |
Benedetta Baldari Simona Vittorio Francesco Sessa Luigi Cipolloni Giuseppe Bertozzi Margherita Neri Santina Cantatore Vittorio Fineschi Mariarosaria Aromatario |
author_sort |
Benedetta Baldari |
title |
Forensic Application of Monoclonal Anti-Human Glycophorin A Antibody in Samples from Decomposed Bodies to Establish Vitality of the Injuries. A Preliminary Experimental Study |
title_short |
Forensic Application of Monoclonal Anti-Human Glycophorin A Antibody in Samples from Decomposed Bodies to Establish Vitality of the Injuries. A Preliminary Experimental Study |
title_full |
Forensic Application of Monoclonal Anti-Human Glycophorin A Antibody in Samples from Decomposed Bodies to Establish Vitality of the Injuries. A Preliminary Experimental Study |
title_fullStr |
Forensic Application of Monoclonal Anti-Human Glycophorin A Antibody in Samples from Decomposed Bodies to Establish Vitality of the Injuries. A Preliminary Experimental Study |
title_full_unstemmed |
Forensic Application of Monoclonal Anti-Human Glycophorin A Antibody in Samples from Decomposed Bodies to Establish Vitality of the Injuries. A Preliminary Experimental Study |
title_sort |
forensic application of monoclonal anti-human glycophorin a antibody in samples from decomposed bodies to establish vitality of the injuries. a preliminary experimental study |
publisher |
MDPI AG |
series |
Healthcare |
issn |
2227-9032 |
publishDate |
2021-04-01 |
description |
Glycophorins are an important group of red blood cell (RBC) transmembrane proteins. Monoclonal antibodies against GPA are employed in immunohistochemical staining during post-mortem examination: Through this method, it is possible to point out the RBC presence in tissues. This experimental study aims to investigate anti-GPA immunohistochemical staining in order to evaluate the vitality of the lesion from corpses in different decomposition state. Six cases were selected, analyzing autopsies’ documentation performed by the Institute of Legal Medicine of Rome in 2010–2018: four samples of fractured bones and three samples of soft tissues. For the control case, the fracture region of the femur was sampled. The results of the present study confirm the preliminary results of other studies, remarking the importance of the GPA immunohistochemical staining to highlight signs of survival. Moreover, this study suggests that the use of this technique should be routinely applied in cases of corpses with advanced putrefaction phenomena, even when the radiological investigation is performed, the macroscopic investigation is inconclusive, the H&E staining is not reliable. This experimental application demonstrated that the use of monoclonal antibody anti-human GPA on bone fractures and soft tissues could be important to verify whether the lesion is vital or not. |
topic |
forensic pathology glycophorin A investigation vitality |
url |
https://www.mdpi.com/2227-9032/9/5/514 |
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