Differing rates of cholesterol absorption among inbred mouse strains yield differing levels of HDL-cholesterol
Inbred strains of mice with differing susceptibilities to atherosclerosis possess widely varying plasma HDL levels. Cholesterol absorption and lipoprotein formation were compared between atherosclerosis-susceptible, low-HDL C57BL6/J mice and atherosclerosis-resistant, high-HDL FVBN/J mice. [3H]chole...
Main Authors: | , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Elsevier
2013-09-01
|
Series: | Journal of Lipid Research |
Subjects: | |
Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520351403 |
id |
doaj-3d05dd66116e4377a7efd41474ba3322 |
---|---|
record_format |
Article |
spelling |
doaj-3d05dd66116e4377a7efd41474ba33222021-04-28T05:59:25ZengElsevierJournal of Lipid Research0022-22752013-09-0154925152524Differing rates of cholesterol absorption among inbred mouse strains yield differing levels of HDL-cholesterolTimothy J. Sontag0Bijoy Chellan1Godfrey S. Getz2Catherine A. Reardon3To whom correspondence should be addressed; Department of Pathology, University of Chicago, Chicago, ILDepartment of Pathology, University of Chicago, Chicago, ILDepartment of Pathology, University of Chicago, Chicago, ILDepartment of Pathology, University of Chicago, Chicago, ILInbred strains of mice with differing susceptibilities to atherosclerosis possess widely varying plasma HDL levels. Cholesterol absorption and lipoprotein formation were compared between atherosclerosis-susceptible, low-HDL C57BL6/J mice and atherosclerosis-resistant, high-HDL FVBN/J mice. [3H]cholesterol and triglyceride appeared in the plasma of FVB mice gavaged with cholesterol in olive oil at a much higher rate than in C57 mice. The plasma cholesterol was found almost entirely as HDL-cholesterol in both strains. Inhibition of lipoprotein catabolism with Tyloxapol revealed that the difference in the rate of [3H]cholesterol appearance in the plasma was due entirely to a greater rate of chylomicron secretion from the intestine of the FVB mice. Lipid absorption into the 2nd quarter of the small intestine is greater in the FVB mice and indicates that this region may contain the factors that give rise to the differences in absorption observed between the two mouse strains. Additionally, ad libitum feeding prior to cholesterol gavage accentuates the absorption rate differences compared with fasting. The resultant remodeling of the increased levels of chylomicron in the plasma may contribute to increased plasma HDL. Intestinal gene expression analysis reveals several genes that may play a role in these differences, including microsomal triglyceride transfer protein and ABCG8.http://www.sciencedirect.com/science/article/pii/S0022227520351403enterocytetriglyceridelipoproteinchylomicron |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Timothy J. Sontag Bijoy Chellan Godfrey S. Getz Catherine A. Reardon |
spellingShingle |
Timothy J. Sontag Bijoy Chellan Godfrey S. Getz Catherine A. Reardon Differing rates of cholesterol absorption among inbred mouse strains yield differing levels of HDL-cholesterol Journal of Lipid Research enterocyte triglyceride lipoprotein chylomicron |
author_facet |
Timothy J. Sontag Bijoy Chellan Godfrey S. Getz Catherine A. Reardon |
author_sort |
Timothy J. Sontag |
title |
Differing rates of cholesterol absorption among inbred mouse strains yield differing levels of HDL-cholesterol |
title_short |
Differing rates of cholesterol absorption among inbred mouse strains yield differing levels of HDL-cholesterol |
title_full |
Differing rates of cholesterol absorption among inbred mouse strains yield differing levels of HDL-cholesterol |
title_fullStr |
Differing rates of cholesterol absorption among inbred mouse strains yield differing levels of HDL-cholesterol |
title_full_unstemmed |
Differing rates of cholesterol absorption among inbred mouse strains yield differing levels of HDL-cholesterol |
title_sort |
differing rates of cholesterol absorption among inbred mouse strains yield differing levels of hdl-cholesterol |
publisher |
Elsevier |
series |
Journal of Lipid Research |
issn |
0022-2275 |
publishDate |
2013-09-01 |
description |
Inbred strains of mice with differing susceptibilities to atherosclerosis possess widely varying plasma HDL levels. Cholesterol absorption and lipoprotein formation were compared between atherosclerosis-susceptible, low-HDL C57BL6/J mice and atherosclerosis-resistant, high-HDL FVBN/J mice. [3H]cholesterol and triglyceride appeared in the plasma of FVB mice gavaged with cholesterol in olive oil at a much higher rate than in C57 mice. The plasma cholesterol was found almost entirely as HDL-cholesterol in both strains. Inhibition of lipoprotein catabolism with Tyloxapol revealed that the difference in the rate of [3H]cholesterol appearance in the plasma was due entirely to a greater rate of chylomicron secretion from the intestine of the FVB mice. Lipid absorption into the 2nd quarter of the small intestine is greater in the FVB mice and indicates that this region may contain the factors that give rise to the differences in absorption observed between the two mouse strains. Additionally, ad libitum feeding prior to cholesterol gavage accentuates the absorption rate differences compared with fasting. The resultant remodeling of the increased levels of chylomicron in the plasma may contribute to increased plasma HDL. Intestinal gene expression analysis reveals several genes that may play a role in these differences, including microsomal triglyceride transfer protein and ABCG8. |
topic |
enterocyte triglyceride lipoprotein chylomicron |
url |
http://www.sciencedirect.com/science/article/pii/S0022227520351403 |
work_keys_str_mv |
AT timothyjsontag differingratesofcholesterolabsorptionamonginbredmousestrainsyielddifferinglevelsofhdlcholesterol AT bijoychellan differingratesofcholesterolabsorptionamonginbredmousestrainsyielddifferinglevelsofhdlcholesterol AT godfreysgetz differingratesofcholesterolabsorptionamonginbredmousestrainsyielddifferinglevelsofhdlcholesterol AT catherineareardon differingratesofcholesterolabsorptionamonginbredmousestrainsyielddifferinglevelsofhdlcholesterol |
_version_ |
1721504687396487168 |