Potential Osteoporosis Recovery by Deep Sea Water through Bone Regeneration in SAMP8 Mice
The aim of this study is to examine the therapeutic potential of deep sea water (DSW) on osteoporosis. Previously, we have established the ovariectomized senescence-accelerated mice (OVX-SAMP8) and demonstrated strong recovery of osteoporosis by stem cell and platelet-rich plasma (PRP). Deep sea wat...
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Series: | Evidence-Based Complementary and Alternative Medicine |
Online Access: | http://dx.doi.org/10.1155/2013/161976 |
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doaj-3d26d960407b4aa69ff983727e4ca1582020-11-24T21:15:54ZengHindawi LimitedEvidence-Based Complementary and Alternative Medicine1741-427X1741-42882013-01-01201310.1155/2013/161976161976Potential Osteoporosis Recovery by Deep Sea Water through Bone Regeneration in SAMP8 MiceHen-Yu Liu0Ming-Che Liu1Ming-Fu Wang2Wei-Hong Chen3Ching-Yu Tsai4Kuan-Hsien Wu5Che-Tong Lin6Ying-Hua Shieh7Rong Zeng8Win-Ping Deng9Stem Cell Research Center, Taipei Medical University, Taipei, TaiwanSchool of Dentistry, College of Oral Medicine, Taipei Medical University, Taipei, TaiwanDepartment of Food and Nutrition, Providence University, Taichung, TaiwanStem Cell Research Center, Taipei Medical University, Taipei, TaiwanStem Cell Research Center, Taipei Medical University, Taipei, TaiwanStem Cell Research Center, Taipei Medical University, Taipei, TaiwanGraduate Institute of Oral Rehabilitation Sciences, Taipei Medical University, Taipei, TaiwanDepartment of Family Medicine, Taipei Medical University, Wan Fang Hospital, Taipei, TaiwanDepartment of Orthopedic Surgery, The Affiliated Hospital, Guangdong Medical College, Zhanjiang 524001, ChinaStem Cell Research Center, Taipei Medical University, Taipei, TaiwanThe aim of this study is to examine the therapeutic potential of deep sea water (DSW) on osteoporosis. Previously, we have established the ovariectomized senescence-accelerated mice (OVX-SAMP8) and demonstrated strong recovery of osteoporosis by stem cell and platelet-rich plasma (PRP). Deep sea water at hardness (HD) 1000 showed significant increase in proliferation of osteoblastic cell (MC3T3) by MTT assay. For in vivo animal study, bone mineral density (BMD) was strongly enhanced followed by the significantly increased trabecular numbers through micro-CT examination after a 4-month deep sea water treatment, and biochemistry analysis showed that serum alkaline phosphatase (ALP) activity was decreased. For stage-specific osteogenesis, bone marrow-derived stromal cells (BMSCs) were harvested and examined. Deep sea water-treated BMSCs showed stronger osteogenic differentiation such as BMP2, RUNX2, OPN, and OCN, and enhanced colony forming abilities, compared to the control group. Interestingly, most untreated OVX-SAMP8 mice died around 10 months; however, approximately 57% of DSW-treated groups lived up to 16.6 months, a life expectancy similar to the previously reported life expectancy for SAMR1 24 months. The results demonstrated the regenerative potentials of deep sea water on osteogenesis, showing that deep sea water could potentially be applied in osteoporosis therapy as a complementary and alternative medicine (CAM).http://dx.doi.org/10.1155/2013/161976 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Hen-Yu Liu Ming-Che Liu Ming-Fu Wang Wei-Hong Chen Ching-Yu Tsai Kuan-Hsien Wu Che-Tong Lin Ying-Hua Shieh Rong Zeng Win-Ping Deng |
spellingShingle |
Hen-Yu Liu Ming-Che Liu Ming-Fu Wang Wei-Hong Chen Ching-Yu Tsai Kuan-Hsien Wu Che-Tong Lin Ying-Hua Shieh Rong Zeng Win-Ping Deng Potential Osteoporosis Recovery by Deep Sea Water through Bone Regeneration in SAMP8 Mice Evidence-Based Complementary and Alternative Medicine |
author_facet |
Hen-Yu Liu Ming-Che Liu Ming-Fu Wang Wei-Hong Chen Ching-Yu Tsai Kuan-Hsien Wu Che-Tong Lin Ying-Hua Shieh Rong Zeng Win-Ping Deng |
author_sort |
Hen-Yu Liu |
title |
Potential Osteoporosis Recovery by Deep Sea Water through Bone Regeneration in SAMP8 Mice |
title_short |
Potential Osteoporosis Recovery by Deep Sea Water through Bone Regeneration in SAMP8 Mice |
title_full |
Potential Osteoporosis Recovery by Deep Sea Water through Bone Regeneration in SAMP8 Mice |
title_fullStr |
Potential Osteoporosis Recovery by Deep Sea Water through Bone Regeneration in SAMP8 Mice |
title_full_unstemmed |
Potential Osteoporosis Recovery by Deep Sea Water through Bone Regeneration in SAMP8 Mice |
title_sort |
potential osteoporosis recovery by deep sea water through bone regeneration in samp8 mice |
publisher |
Hindawi Limited |
series |
Evidence-Based Complementary and Alternative Medicine |
issn |
1741-427X 1741-4288 |
publishDate |
2013-01-01 |
description |
The aim of this study is to examine the therapeutic potential of deep sea water (DSW) on osteoporosis. Previously, we have established the ovariectomized senescence-accelerated mice (OVX-SAMP8) and demonstrated strong recovery of osteoporosis by stem cell and platelet-rich plasma (PRP). Deep sea water at hardness (HD) 1000 showed significant increase in proliferation of osteoblastic cell (MC3T3) by MTT assay. For in vivo animal study, bone mineral density (BMD) was strongly enhanced followed by the significantly increased trabecular numbers through micro-CT examination after a 4-month deep sea water treatment, and biochemistry analysis showed that serum alkaline phosphatase (ALP) activity was decreased. For stage-specific osteogenesis, bone marrow-derived stromal cells (BMSCs) were harvested and examined. Deep sea water-treated BMSCs showed stronger osteogenic differentiation such as BMP2, RUNX2, OPN, and OCN, and enhanced colony forming abilities, compared to the control group. Interestingly, most untreated OVX-SAMP8 mice died around 10 months; however, approximately 57% of DSW-treated groups lived up to 16.6 months, a life expectancy similar to the previously reported life expectancy for SAMR1 24 months. The results demonstrated the regenerative potentials of deep sea water on osteogenesis, showing that deep sea water could potentially be applied in osteoporosis therapy as a complementary and alternative medicine (CAM). |
url |
http://dx.doi.org/10.1155/2013/161976 |
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