Disclosing bias in bisulfite assay: MethPrimers underestimate high DNA methylation.
Discordant results obtained in bisulfite assays using MethPrimers (PCR primers designed using MethPrimer software or assuming that non-CpGs cytosines are non methylated) versus primers insensitive to cytosine methylation lead us to hypothesize a technical bias. We therefore used the two kinds of pri...
Main Authors: | Andrea Fuso, Giampiero Ferraguti, Sigfrido Scarpa, Isidre Ferrer, Marco Lucarelli |
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Format: | Article |
Language: | English |
Published: |
Public Library of Science (PLoS)
2015-01-01
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Series: | PLoS ONE |
Online Access: | http://europepmc.org/articles/PMC4333220?pdf=render |
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