Serosurvey of Human Antibodies Recognizing Aedes aegypti D7 Salivary Proteins in Colombia
BackgroundDengue is one of the most geographically significant mosquito-borne viral diseases transmitted by Aedes mosquitoes. During blood feeding, mosquitoes deposit salivary proteins that induce antibody responses. These can be related to the intensity of exposure to bites. Some mosquito salivary...
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doaj-3d3f3d345cef4ae2b87b3a64e09b76002020-11-25T00:18:26ZengFrontiers Media S.A.Frontiers in Public Health2296-25652018-05-01610.3389/fpubh.2018.00111355246Serosurvey of Human Antibodies Recognizing Aedes aegypti D7 Salivary Proteins in ColombiaBerlin L. Londono-Renteria0Heman Shakeri1Paula Rozo-Lopez2Michael J. Conway3Natasha Duggan4Majid Jaberi-Douraki5Majid Jaberi-Douraki6Tonya M. Colpitts7Department of Entomology, Kansas State University, Manhattan, KS, United StatesDepartment of Anatomy and Physiology, Institute of Computational Comparative Medicine, Kansas State University, Manhattan, KS, United StatesDepartment of Entomology, Kansas State University, Manhattan, KS, United StatesCentral Michigan University College of Medicine, Mount Pleasant, MI, United StatesDepartment of Cell Biology, University of Miami Miller School of Medicine, Miami, FL, United StatesDepartment of Anatomy and Physiology, Institute of Computational Comparative Medicine, Kansas State University, Manhattan, KS, United StatesDepartment of Mathematics, Institute of Computational Comparative Medicine, Kansas State University, Manhattan, KS, United StatesNational Emerging Infectious Diseases Laboratories (NEIDL), Department of Microbiology, Boston University School of Medicine, Boston, MA, United StatesBackgroundDengue is one of the most geographically significant mosquito-borne viral diseases transmitted by Aedes mosquitoes. During blood feeding, mosquitoes deposit salivary proteins that induce antibody responses. These can be related to the intensity of exposure to bites. Some mosquito salivary proteins, such as D7 proteins, are known as potent allergens. The antibody response to D7 proteins can be used as a marker to evaluate the risk of exposure and disease transmission and provide critical information for understanding the dynamics of vector–host interactions.MethodsThe study was conducted at the Los Patios Hospital, Cucuta, Norte de Santander, Colombia. A total of 63 participants were enrolled in the study. Participants were categorized into three disease status groups, age groups, and socioeconomic strata. The level of IgG antibodies against D7 Aedes proteins was determined by ELISA. We used a statistical approach to determine if there is an association between antibody levels and factors such as age, living conditions, and dengue virus (DENV) infection.ResultsWe found that IgG antibodies against D7 proteins were higher in non-DENV infected individuals in comparison to DENV-infected participants. Also, the age factor showed a significant positive correlation with IgG antibodies against D7 proteins, and the living conditions (socioeconomic stratification), in people aged 20 years or older, are a statistically significant factor in the variability of IgG antibodies against D7 proteins.ConclusionThis pilot study represents the first approximation to elucidate any correlation between the antibody response against mosquito D7 salivary proteins and its correlation with age, living conditions, and DENV infection in a dengue endemic area.https://www.frontiersin.org/article/10.3389/fpubh.2018.00111/fullAedes salivaD7antibody levelsbiomarkerdengue |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Berlin L. Londono-Renteria Heman Shakeri Paula Rozo-Lopez Michael J. Conway Natasha Duggan Majid Jaberi-Douraki Majid Jaberi-Douraki Tonya M. Colpitts |
spellingShingle |
Berlin L. Londono-Renteria Heman Shakeri Paula Rozo-Lopez Michael J. Conway Natasha Duggan Majid Jaberi-Douraki Majid Jaberi-Douraki Tonya M. Colpitts Serosurvey of Human Antibodies Recognizing Aedes aegypti D7 Salivary Proteins in Colombia Frontiers in Public Health Aedes saliva D7 antibody levels biomarker dengue |
author_facet |
Berlin L. Londono-Renteria Heman Shakeri Paula Rozo-Lopez Michael J. Conway Natasha Duggan Majid Jaberi-Douraki Majid Jaberi-Douraki Tonya M. Colpitts |
author_sort |
Berlin L. Londono-Renteria |
title |
Serosurvey of Human Antibodies Recognizing Aedes aegypti D7 Salivary Proteins in Colombia |
title_short |
Serosurvey of Human Antibodies Recognizing Aedes aegypti D7 Salivary Proteins in Colombia |
title_full |
Serosurvey of Human Antibodies Recognizing Aedes aegypti D7 Salivary Proteins in Colombia |
title_fullStr |
Serosurvey of Human Antibodies Recognizing Aedes aegypti D7 Salivary Proteins in Colombia |
title_full_unstemmed |
Serosurvey of Human Antibodies Recognizing Aedes aegypti D7 Salivary Proteins in Colombia |
title_sort |
serosurvey of human antibodies recognizing aedes aegypti d7 salivary proteins in colombia |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Public Health |
issn |
2296-2565 |
publishDate |
2018-05-01 |
description |
BackgroundDengue is one of the most geographically significant mosquito-borne viral diseases transmitted by Aedes mosquitoes. During blood feeding, mosquitoes deposit salivary proteins that induce antibody responses. These can be related to the intensity of exposure to bites. Some mosquito salivary proteins, such as D7 proteins, are known as potent allergens. The antibody response to D7 proteins can be used as a marker to evaluate the risk of exposure and disease transmission and provide critical information for understanding the dynamics of vector–host interactions.MethodsThe study was conducted at the Los Patios Hospital, Cucuta, Norte de Santander, Colombia. A total of 63 participants were enrolled in the study. Participants were categorized into three disease status groups, age groups, and socioeconomic strata. The level of IgG antibodies against D7 Aedes proteins was determined by ELISA. We used a statistical approach to determine if there is an association between antibody levels and factors such as age, living conditions, and dengue virus (DENV) infection.ResultsWe found that IgG antibodies against D7 proteins were higher in non-DENV infected individuals in comparison to DENV-infected participants. Also, the age factor showed a significant positive correlation with IgG antibodies against D7 proteins, and the living conditions (socioeconomic stratification), in people aged 20 years or older, are a statistically significant factor in the variability of IgG antibodies against D7 proteins.ConclusionThis pilot study represents the first approximation to elucidate any correlation between the antibody response against mosquito D7 salivary proteins and its correlation with age, living conditions, and DENV infection in a dengue endemic area. |
topic |
Aedes saliva D7 antibody levels biomarker dengue |
url |
https://www.frontiersin.org/article/10.3389/fpubh.2018.00111/full |
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