Differential expression between drought-tolerant and drought-sensitive sugarcane under mild and moderate water stress as revealed by a comparative analysis of leaf transcriptome

• Main Authors: Wanapinun Nawae, Jeremy R. Shearman, Sithichoke Tangphatsornruang, Prapat Punpee, Thippawan Yoocha, Duangjai Sangsrakru, Chaiwat Naktang, Chutima Sonthirod, Warodom Wirojsirasak, Kittipat Ukoskit, Klanarong Sriroth, Peeraya Klomsa-ard, Wirulda Pootakham
• Format: Article
• Language: English
• Published: PeerJ Inc. 2020-07-01
• Series: PeerJ
• Subjects: Sugarcane; Drought; Drought tolerance; Early response; Transcriptome; RNA-Seq
• Online Access: https://peerj.com/articles/9608.pdf

Sugarcane contributes 80% of global sugar production and to bioethanol generation for the bioenergy industry. Its productivity is threatened by drought that can cause up to 60% yield loss. This study used RNA-Seq to gain a better understanding of the underlying mechanism by which drought-tolerant sugarcane copes with water stress. We compared gene expression in KPS01-12 (drought-tolerant genotype) and UT12 (drought-sensitive genotype) that have significantly different yield loss rates under drought conditions. We treated KPS01-12 and UT12 with mild and moderate water stress and found differentially expressed genes in various biological processes. KPS01-12 had higher expression of genes that were involved in water retention, antioxidant secondary metabolite biosynthesis, and oxidative and osmotic stress response than UT12. In contrast, the sensitive genotype had more down-regulated genes that were involved in photosynthesis, carbon fixation and Calvin cycle than the tolerant genotype. Our obtained expression profiles suggest that the tolerant sugarcane has a more effective genetic response than the sensitive genotype at the initiation of drought stress. The knowledge gained from this study may be applied in breeding programs to improve sugarcane production in drought conditions.