Evaluation of the inhibitory effect of ivermectin on the growth of Babesia and Theileria parasites in vitro and in vivo
Abstract Background Treatment is the principle way to control and eliminate piroplasmosis. The search for new chemotherapy against Babesia and Theileria has become increasingly urgent due to parasite resistance to current drugs. Ivermectin (IVM) was the world’s first endectocide, capable of killing...
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doaj-3d70731b7b0444a492769c0b40419bc02020-11-25T03:02:39ZengBMCTropical Medicine and Health1349-41472019-07-0147111210.1186/s41182-019-0171-8Evaluation of the inhibitory effect of ivermectin on the growth of Babesia and Theileria parasites in vitro and in vivoGaber El-Saber Batiha0Amani Magdy Beshbishy1Dickson Stuart Tayebwa2Oluyomi Stephen Adeyemi3Naoaki Yokoyama4Ikuo Igarashi5National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary MedicineNational Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary MedicineNational Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary MedicineMedicinal Biochemistry, Nanomedicine and Toxicology Laboratory, Department of Biological Sciences, Landmark UniversityNational Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary MedicineNational Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary MedicineAbstract Background Treatment is the principle way to control and eliminate piroplasmosis. The search for new chemotherapy against Babesia and Theileria has become increasingly urgent due to parasite resistance to current drugs. Ivermectin (IVM) was the world’s first endectocide, capable of killing a wide variety of parasites and vectors, both inside and outside the body. It is currently authorized to treat onchocerciasis, lymphatic filariasis, strongyloidiasis, and scabies. The current study documented the efficacy of IVM on the growth of Babesia and Theileria in vitro and in vivo. Methods The fluorescence-based assay was used for evaluating the inhibitory effect of IVM on four Babesia species, including B. bovis, B. bigemina, B. divergens, B. caballi, and Theileria equi, the combination with diminazene aceturate (DA), clofazimine (CF), and atovaquone (AQ) on in vitro cultures, and on the multiplication of a B. microti-infected mouse model. The cytotoxicity of compounds was tested on Madin–Darby bovine kidney (MDBK), mouse embryonic fibroblast (NIH/3 T3), and human foreskin fibroblast (HFF) cell lines. Results The half-maximal inhibitory concentration (IC50) values determined for IVM against B. bovis, B. bigemina, B. divergens, B. caballi, and T. equi were 53.3 ± 4.8, 98.6 ± 5.7, 30.1 ± 2.2, 43.7 ± 3.7, and 90.1 ± 8.1 μM, respectively. Toxicity assays on MDBK, NIH/3 T3, and HFF cell lines showed that IVM affected the viability of cells with a half-maximal effective concentration (EC50) of 138.9 ± 4.9, 283.8 ± 3.6, and 287.5 ± 7.6 μM, respectively. In the in vivo experiment, IVM, when administered intraperitoneally at 4 mg/kg, significantly (p < 0.05) inhibited the growth of B. microti in mice by 63%. Furthermore, combination therapies of IVM–DA, IVM–AQ, and IVM–CF at a half dose reduced the peak parasitemia of B. microti by 83.7%, 76.5%, and 74.4%, respectively. Moreover, this study confirmed the absence of B. microti DNA in groups treated with combination chemotherapy of IVM + DA and IVM + AQ 49 days after infection. Conclusions These findings suggest that IVM has the potential to be an alternative remedy for treating piroplasmosis.http://link.springer.com/article/10.1186/s41182-019-0171-8IvermectinBabesiaTheileriaIn vitroIn vivo |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Gaber El-Saber Batiha Amani Magdy Beshbishy Dickson Stuart Tayebwa Oluyomi Stephen Adeyemi Naoaki Yokoyama Ikuo Igarashi |
spellingShingle |
Gaber El-Saber Batiha Amani Magdy Beshbishy Dickson Stuart Tayebwa Oluyomi Stephen Adeyemi Naoaki Yokoyama Ikuo Igarashi Evaluation of the inhibitory effect of ivermectin on the growth of Babesia and Theileria parasites in vitro and in vivo Tropical Medicine and Health Ivermectin Babesia Theileria In vitro In vivo |
author_facet |
Gaber El-Saber Batiha Amani Magdy Beshbishy Dickson Stuart Tayebwa Oluyomi Stephen Adeyemi Naoaki Yokoyama Ikuo Igarashi |
author_sort |
Gaber El-Saber Batiha |
title |
Evaluation of the inhibitory effect of ivermectin on the growth of Babesia and Theileria parasites in vitro and in vivo |
title_short |
Evaluation of the inhibitory effect of ivermectin on the growth of Babesia and Theileria parasites in vitro and in vivo |
title_full |
Evaluation of the inhibitory effect of ivermectin on the growth of Babesia and Theileria parasites in vitro and in vivo |
title_fullStr |
Evaluation of the inhibitory effect of ivermectin on the growth of Babesia and Theileria parasites in vitro and in vivo |
title_full_unstemmed |
Evaluation of the inhibitory effect of ivermectin on the growth of Babesia and Theileria parasites in vitro and in vivo |
title_sort |
evaluation of the inhibitory effect of ivermectin on the growth of babesia and theileria parasites in vitro and in vivo |
publisher |
BMC |
series |
Tropical Medicine and Health |
issn |
1349-4147 |
publishDate |
2019-07-01 |
description |
Abstract Background Treatment is the principle way to control and eliminate piroplasmosis. The search for new chemotherapy against Babesia and Theileria has become increasingly urgent due to parasite resistance to current drugs. Ivermectin (IVM) was the world’s first endectocide, capable of killing a wide variety of parasites and vectors, both inside and outside the body. It is currently authorized to treat onchocerciasis, lymphatic filariasis, strongyloidiasis, and scabies. The current study documented the efficacy of IVM on the growth of Babesia and Theileria in vitro and in vivo. Methods The fluorescence-based assay was used for evaluating the inhibitory effect of IVM on four Babesia species, including B. bovis, B. bigemina, B. divergens, B. caballi, and Theileria equi, the combination with diminazene aceturate (DA), clofazimine (CF), and atovaquone (AQ) on in vitro cultures, and on the multiplication of a B. microti-infected mouse model. The cytotoxicity of compounds was tested on Madin–Darby bovine kidney (MDBK), mouse embryonic fibroblast (NIH/3 T3), and human foreskin fibroblast (HFF) cell lines. Results The half-maximal inhibitory concentration (IC50) values determined for IVM against B. bovis, B. bigemina, B. divergens, B. caballi, and T. equi were 53.3 ± 4.8, 98.6 ± 5.7, 30.1 ± 2.2, 43.7 ± 3.7, and 90.1 ± 8.1 μM, respectively. Toxicity assays on MDBK, NIH/3 T3, and HFF cell lines showed that IVM affected the viability of cells with a half-maximal effective concentration (EC50) of 138.9 ± 4.9, 283.8 ± 3.6, and 287.5 ± 7.6 μM, respectively. In the in vivo experiment, IVM, when administered intraperitoneally at 4 mg/kg, significantly (p < 0.05) inhibited the growth of B. microti in mice by 63%. Furthermore, combination therapies of IVM–DA, IVM–AQ, and IVM–CF at a half dose reduced the peak parasitemia of B. microti by 83.7%, 76.5%, and 74.4%, respectively. Moreover, this study confirmed the absence of B. microti DNA in groups treated with combination chemotherapy of IVM + DA and IVM + AQ 49 days after infection. Conclusions These findings suggest that IVM has the potential to be an alternative remedy for treating piroplasmosis. |
topic |
Ivermectin Babesia Theileria In vitro In vivo |
url |
http://link.springer.com/article/10.1186/s41182-019-0171-8 |
work_keys_str_mv |
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