Pro and Anti-inflammatory Cytokine Production in CD4+ T Lymphocytes in Children with Asthma and Allergic Rhinitis Exposed to the Monocyte Locomotion Inhibitory Factor (MLIF)
Entamoeba histolytica produces, in axenic culture, the monocytes locomotion inhibitory factor (MLIF), a oligopeptide with selective anti-inflammatory properties. We evaluated the effect of MLIF on the expression of pro- and anti-inflammatory cytokines in CD4+ T lymphocytes from children with asthma...
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Tehran University of Medical Sciences
2018-08-01
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doaj-3d70fa3c6b9c4df58cf1ab71656bd1a42020-11-25T04:11:48ZengTehran University of Medical SciencesIranian Journal of Allergy, Asthma and Immunology1735-15021735-52492018-08-0117410.18502/ijaai.v17i4.981629Pro and Anti-inflammatory Cytokine Production in CD4+ T Lymphocytes in Children with Asthma and Allergic Rhinitis Exposed to the Monocyte Locomotion Inhibitory Factor (MLIF)Sara Rojas-Dotor0Adriana González-Hernández1Francisco León-Aguilar2Víctor Juárez-Téllez3Patricia Gómez de León-Cruces4Departamento de Salud Pública, Facultad de Medicina Universidad Nacional Autónoma de México, México City, MéxicoUnidad de Medicina Familiar No. 7, Instituto Mexicano del Seguro Social, México City, MéxicoUnidad de Medicina Familiar No. 7, Instituto Mexicano del Seguro Social, México City, MéxicoUnidad de Medicina Familiar No. 7, Instituto Mexicano del Seguro Social, México City, MéxicoDepartamento de Salud Pública, Facultad de Medicina Universidad Nacional Autónoma de México, México City, México Entamoeba histolytica produces, in axenic culture, the monocytes locomotion inhibitory factor (MLIF), a oligopeptide with selective anti-inflammatory properties. We evaluated the effect of MLIF on the expression of pro- and anti-inflammatory cytokines in CD4+ T lymphocytes from children with asthma and allergic rhinitis. Twelve children with severe asthma, 12 children with allergic rhinitis and 6 healthy controls were recruited for this study between May and December 2016. CD4+ T cells were cultured for 24 h at 37°C, 5% CO2 in the presence of MLIF, 1-phorbol 12-myristate 13-acetate (PMA), MLIF+PMA or RPMI. Interleukin-10 (IL-10), IL-4, interferon gamma (IFN-γ) and tumor necrosis factor alpha (TNF-α) expression levels were measured in the supernatants of T-cell cultures using the enzyme-linked immunosorbent assay (ELISA). Pro- and anti-inflammatory cytokines were inhibited by MLIF (IFN-γ p=0.0036, TNF-α p<0.001, IL-4 p=0.0082) in asthmatic patients, however IFN-γ was not significantly inhibited (NS) in patients with allergic rhinitis when compared to the RPMI group. In CD4+ T cells treated with PMA+MLIF, the expression levels of IFN-γ, TNF-α and IL-4 were strongly inhibited (p<0.001, p<0.001 and p<0.0094), compared to PMA treatment alone, for both, rhinitis and asthma. IL-10 expression was not affected by MLIF in neither of the two diseases. We conclude that MLIF alters the pro/anti-inflammatory balance and induces inhibition of IL-4, IFN-γ and TNF-α, but does not affect IL-10. https://ijaai.tums.ac.ir/index.php/ijaai/article/view/1629AsthmaInflammationLymphocytesMonocytes locomotion inhibitory factorRhinitis |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Sara Rojas-Dotor Adriana González-Hernández Francisco León-Aguilar Víctor Juárez-Téllez Patricia Gómez de León-Cruces |
spellingShingle |
Sara Rojas-Dotor Adriana González-Hernández Francisco León-Aguilar Víctor Juárez-Téllez Patricia Gómez de León-Cruces Pro and Anti-inflammatory Cytokine Production in CD4+ T Lymphocytes in Children with Asthma and Allergic Rhinitis Exposed to the Monocyte Locomotion Inhibitory Factor (MLIF) Iranian Journal of Allergy, Asthma and Immunology Asthma Inflammation Lymphocytes Monocytes locomotion inhibitory factor Rhinitis |
author_facet |
Sara Rojas-Dotor Adriana González-Hernández Francisco León-Aguilar Víctor Juárez-Téllez Patricia Gómez de León-Cruces |
author_sort |
Sara Rojas-Dotor |
title |
Pro and Anti-inflammatory Cytokine Production in CD4+ T Lymphocytes in Children with Asthma and Allergic Rhinitis Exposed to the Monocyte Locomotion Inhibitory Factor (MLIF) |
title_short |
Pro and Anti-inflammatory Cytokine Production in CD4+ T Lymphocytes in Children with Asthma and Allergic Rhinitis Exposed to the Monocyte Locomotion Inhibitory Factor (MLIF) |
title_full |
Pro and Anti-inflammatory Cytokine Production in CD4+ T Lymphocytes in Children with Asthma and Allergic Rhinitis Exposed to the Monocyte Locomotion Inhibitory Factor (MLIF) |
title_fullStr |
Pro and Anti-inflammatory Cytokine Production in CD4+ T Lymphocytes in Children with Asthma and Allergic Rhinitis Exposed to the Monocyte Locomotion Inhibitory Factor (MLIF) |
title_full_unstemmed |
Pro and Anti-inflammatory Cytokine Production in CD4+ T Lymphocytes in Children with Asthma and Allergic Rhinitis Exposed to the Monocyte Locomotion Inhibitory Factor (MLIF) |
title_sort |
pro and anti-inflammatory cytokine production in cd4+ t lymphocytes in children with asthma and allergic rhinitis exposed to the monocyte locomotion inhibitory factor (mlif) |
publisher |
Tehran University of Medical Sciences |
series |
Iranian Journal of Allergy, Asthma and Immunology |
issn |
1735-1502 1735-5249 |
publishDate |
2018-08-01 |
description |
Entamoeba histolytica produces, in axenic culture, the monocytes locomotion inhibitory factor (MLIF), a oligopeptide with selective anti-inflammatory properties. We evaluated the effect of MLIF on the expression of pro- and anti-inflammatory cytokines in CD4+ T lymphocytes from children with asthma and allergic rhinitis. Twelve children with severe asthma, 12 children with allergic rhinitis and 6 healthy controls were recruited for this study between May and December 2016. CD4+ T cells were cultured for 24 h at 37°C, 5% CO2 in the presence of MLIF, 1-phorbol 12-myristate 13-acetate (PMA), MLIF+PMA or RPMI. Interleukin-10 (IL-10), IL-4, interferon gamma (IFN-γ) and tumor necrosis factor alpha (TNF-α) expression levels were measured in the supernatants of T-cell cultures using the enzyme-linked immunosorbent assay (ELISA). Pro- and anti-inflammatory cytokines were inhibited by MLIF (IFN-γ p=0.0036, TNF-α p<0.001, IL-4 p=0.0082) in asthmatic patients, however IFN-γ was not significantly inhibited (NS) in patients with allergic rhinitis when compared to the RPMI group. In CD4+ T cells treated with PMA+MLIF, the expression levels of IFN-γ, TNF-α and IL-4 were strongly inhibited (p<0.001, p<0.001 and p<0.0094), compared to PMA treatment alone, for both, rhinitis and asthma. IL-10 expression was not affected by MLIF in neither of the two diseases. We conclude that MLIF alters the pro/anti-inflammatory balance and induces inhibition of IL-4, IFN-γ and TNF-α, but does not affect IL-10.
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topic |
Asthma Inflammation Lymphocytes Monocytes locomotion inhibitory factor Rhinitis |
url |
https://ijaai.tums.ac.ir/index.php/ijaai/article/view/1629 |
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