A study of genetic population of Alosa braschnicowi (Borodin, 1904) in Sari and Mahmodabad coasts in the Caspian Sea, using Microsatellite loci

In this study, five Microsatellite loci were used to evaluate genetic diversity of A. braschnicowi between two populations of the Caspian Sea. Sixty samples were collected from the coasts of Mahmodabad and Sari (30 specimens for each population). Five Microsatellite loci were highly polymorphic amon...

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Main Authors: Omid Jafari, Ali Shabany, Hamed Kolangi Miandare
Format: Article
Language:English
Published: University of Tehran 2014-02-01
Series:International Journal of Aquatic Biology
Subjects:
Online Access:http://ij-aquaticbiology.com/index.php/ijab/article/view/19
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spelling doaj-3d79385a0c3e44488a7a845f59ed7c682020-11-25T02:53:13ZengUniversity of TehranInternational Journal of Aquatic Biology2383-09562322-52702014-02-0121202619A study of genetic population of Alosa braschnicowi (Borodin, 1904) in Sari and Mahmodabad coasts in the Caspian Sea, using Microsatellite lociOmid JafariAli ShabanyHamed Kolangi MiandareIn this study, five Microsatellite loci were used to evaluate genetic diversity of A. braschnicowi between two populations of the Caspian Sea. Sixty samples were collected from the coasts of Mahmodabad and Sari (30 specimens for each population). Five Microsatellite loci were highly polymorphic among all samples. The number of alleles per locus observed ranged from 17 to 32 and averaged 14.1 alleles across two populations. The average observed heterozygosis in Mahmodabad and Sari were 57.1% to 60.1% and average observed heterozygosis between two populations was 58.9%. Among 10 population-locus (5 loci × 2 populations) only two tests were in the Hardy-Weinberg equilibrium, so highly deviation from the Hardy-Weinberg equilibrium was found. The average values of Fis and Nm were 0.33 and 14.19, respectively. Also AMOVA on base Fst index showed low genetic difference between two populations (2%), while the genetic diversity within population was 98%. Due to allelic diversity and estimates of heterozygosity, these markers can be useful in Alosa genus for population level analysis in the Caspian Sea.http://ij-aquaticbiology.com/index.php/ijab/article/view/19Alosa braschnicowi, Caspian Sea, Microsatellites, Molecular marker, Polymorphism
collection DOAJ
language English
format Article
sources DOAJ
author Omid Jafari
Ali Shabany
Hamed Kolangi Miandare
spellingShingle Omid Jafari
Ali Shabany
Hamed Kolangi Miandare
A study of genetic population of Alosa braschnicowi (Borodin, 1904) in Sari and Mahmodabad coasts in the Caspian Sea, using Microsatellite loci
International Journal of Aquatic Biology
Alosa braschnicowi, Caspian Sea, Microsatellites, Molecular marker, Polymorphism
author_facet Omid Jafari
Ali Shabany
Hamed Kolangi Miandare
author_sort Omid Jafari
title A study of genetic population of Alosa braschnicowi (Borodin, 1904) in Sari and Mahmodabad coasts in the Caspian Sea, using Microsatellite loci
title_short A study of genetic population of Alosa braschnicowi (Borodin, 1904) in Sari and Mahmodabad coasts in the Caspian Sea, using Microsatellite loci
title_full A study of genetic population of Alosa braschnicowi (Borodin, 1904) in Sari and Mahmodabad coasts in the Caspian Sea, using Microsatellite loci
title_fullStr A study of genetic population of Alosa braschnicowi (Borodin, 1904) in Sari and Mahmodabad coasts in the Caspian Sea, using Microsatellite loci
title_full_unstemmed A study of genetic population of Alosa braschnicowi (Borodin, 1904) in Sari and Mahmodabad coasts in the Caspian Sea, using Microsatellite loci
title_sort study of genetic population of alosa braschnicowi (borodin, 1904) in sari and mahmodabad coasts in the caspian sea, using microsatellite loci
publisher University of Tehran
series International Journal of Aquatic Biology
issn 2383-0956
2322-5270
publishDate 2014-02-01
description In this study, five Microsatellite loci were used to evaluate genetic diversity of A. braschnicowi between two populations of the Caspian Sea. Sixty samples were collected from the coasts of Mahmodabad and Sari (30 specimens for each population). Five Microsatellite loci were highly polymorphic among all samples. The number of alleles per locus observed ranged from 17 to 32 and averaged 14.1 alleles across two populations. The average observed heterozygosis in Mahmodabad and Sari were 57.1% to 60.1% and average observed heterozygosis between two populations was 58.9%. Among 10 population-locus (5 loci × 2 populations) only two tests were in the Hardy-Weinberg equilibrium, so highly deviation from the Hardy-Weinberg equilibrium was found. The average values of Fis and Nm were 0.33 and 14.19, respectively. Also AMOVA on base Fst index showed low genetic difference between two populations (2%), while the genetic diversity within population was 98%. Due to allelic diversity and estimates of heterozygosity, these markers can be useful in Alosa genus for population level analysis in the Caspian Sea.
topic Alosa braschnicowi, Caspian Sea, Microsatellites, Molecular marker, Polymorphism
url http://ij-aquaticbiology.com/index.php/ijab/article/view/19
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