Study of T Cell subsets and IL-7 protein expression in HIV-1-infected patients after 7 years HAART
<p>Abstract</p> <p>Objective</p> <p>To study the changes in T cell subsets and IL-7 in HIV-1-infected patients after seven years of highly active antiretroviral therapy (HAART).</p> <p>Methods</p> <p>Seventy-five individuals were included in this...
Main Authors: | , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
BMC
2011-11-01
|
Series: | European Journal of Medical Research |
Subjects: | |
Online Access: | http://www.eurjmedres.com/content/16/11/473 |
id |
doaj-3d8a9f69c9114e56a697a31c05470f69 |
---|---|
record_format |
Article |
spelling |
doaj-3d8a9f69c9114e56a697a31c05470f692020-11-24T20:48:01ZengBMCEuropean Journal of Medical Research2047-783X2011-11-01161147310.1186/2047-783X-16-11-473Study of T Cell subsets and IL-7 protein expression in HIV-1-infected patients after 7 years HAARTShou CWeng NJin YFeng LJin CHoextermann SPotthoff ASkaletz-Rorowski ABrockmeyer NHWu N<p>Abstract</p> <p>Objective</p> <p>To study the changes in T cell subsets and IL-7 in HIV-1-infected patients after seven years of highly active antiretroviral therapy (HAART).</p> <p>Methods</p> <p>Seventy-five individuals were included in this study (25 with effective HAART, 18 with ineffective HAART, 17 untreated HIV+ patients, and 15 volunteers in the HIV negative control group). The counts of CD4<sup>+</sup>, CD8<sup>+</sup>, CD8/CD38<sup>+</sup>, and CD8/HLADR<sup>+ </sup>T cells as well as the IL-7 protein expression was measured at 5 time points during a period of seven years in patients starting HAART (baseline) and in the HIV negative control group. The expression of CD127 on CD3<sup>+ </sup>T cells was measured by flow cytometry at a single time point (after 7 years) in patients with HAART and was compared with untreated HIV+ patients and the HIV negative control group.</p> <p>Results</p> <p>At baseline CD4<sup>+ </sup>T cell counts of HIV-1-infected patients were lower than that in the control group (p < 0.01), whereas the CD8<sup>+</sup>, CD8/HLADR<sup>+ </sup>and CD8/CD38<sup>+ </sup>T cell counts were higher than those in the control group (<it>p <</it>0.01). After seven years of effective HAART, the CD4<sup>+ </sup>T cell counts had increased and the CD8<sup>+ </sup>T cell count had decreased, although not to the normal levels (<it>p </it>< 0.05). Both the CD8/HLADR<sup>+ </sup>and CD8/CD38<sup>+ </sup>T cell counts had gradually approached those of the control group (<it>p </it>> 0.05). In the ineffective HAART group, the CD8/CD38<sup>+ </sup>T cell count had not decreased significantly, and CD8/HLADR<sup>+ </sup>T cell count gradually decreased. Before treatment, IL-7 serum levels of patients were significantly higher than that in the control group (<it>p </it>< 0.01). After seven years of effective HAART, IL-7 levels had gradually decreased, but were still higher than in the control group (<it>p </it>< 0.01). The CD127 expression on CD3<sup>+ </sup>CD8<sup>+ </sup>T cells in effective HAART patients was higher than in untreated HIV+ patients (<it>p </it>< 0.05), but was lower than that in the control group (<it>p </it>< 0.05). CD127 expression on CD3<sup>+ </sup>CD4+ T cells was not significantly different among the control group, untreated HIV+ patients and effective HAART group.</p> <p>Conclusion</p> <p><it>After seven years of </it>effective HAART, the quantity and capacity of T cell subsets and IL-7 in HIV-1-infected patients had been partially restored, and the abnormal immune activation has significantly diminished.</p> http://www.eurjmedres.com/content/16/11/473HIV-1highly active antiretroviral therapy (HAART)T cell subsetsIL-7 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Shou C Weng N Jin Y Feng L Jin C Hoextermann S Potthoff A Skaletz-Rorowski A Brockmeyer NH Wu N |
spellingShingle |
Shou C Weng N Jin Y Feng L Jin C Hoextermann S Potthoff A Skaletz-Rorowski A Brockmeyer NH Wu N Study of T Cell subsets and IL-7 protein expression in HIV-1-infected patients after 7 years HAART European Journal of Medical Research HIV-1 highly active antiretroviral therapy (HAART) T cell subsets IL-7 |
author_facet |
Shou C Weng N Jin Y Feng L Jin C Hoextermann S Potthoff A Skaletz-Rorowski A Brockmeyer NH Wu N |
author_sort |
Shou C |
title |
Study of T Cell subsets and IL-7 protein expression in HIV-1-infected patients after 7 years HAART |
title_short |
Study of T Cell subsets and IL-7 protein expression in HIV-1-infected patients after 7 years HAART |
title_full |
Study of T Cell subsets and IL-7 protein expression in HIV-1-infected patients after 7 years HAART |
title_fullStr |
Study of T Cell subsets and IL-7 protein expression in HIV-1-infected patients after 7 years HAART |
title_full_unstemmed |
Study of T Cell subsets and IL-7 protein expression in HIV-1-infected patients after 7 years HAART |
title_sort |
study of t cell subsets and il-7 protein expression in hiv-1-infected patients after 7 years haart |
publisher |
BMC |
series |
European Journal of Medical Research |
issn |
2047-783X |
publishDate |
2011-11-01 |
description |
<p>Abstract</p> <p>Objective</p> <p>To study the changes in T cell subsets and IL-7 in HIV-1-infected patients after seven years of highly active antiretroviral therapy (HAART).</p> <p>Methods</p> <p>Seventy-five individuals were included in this study (25 with effective HAART, 18 with ineffective HAART, 17 untreated HIV+ patients, and 15 volunteers in the HIV negative control group). The counts of CD4<sup>+</sup>, CD8<sup>+</sup>, CD8/CD38<sup>+</sup>, and CD8/HLADR<sup>+ </sup>T cells as well as the IL-7 protein expression was measured at 5 time points during a period of seven years in patients starting HAART (baseline) and in the HIV negative control group. The expression of CD127 on CD3<sup>+ </sup>T cells was measured by flow cytometry at a single time point (after 7 years) in patients with HAART and was compared with untreated HIV+ patients and the HIV negative control group.</p> <p>Results</p> <p>At baseline CD4<sup>+ </sup>T cell counts of HIV-1-infected patients were lower than that in the control group (p < 0.01), whereas the CD8<sup>+</sup>, CD8/HLADR<sup>+ </sup>and CD8/CD38<sup>+ </sup>T cell counts were higher than those in the control group (<it>p <</it>0.01). After seven years of effective HAART, the CD4<sup>+ </sup>T cell counts had increased and the CD8<sup>+ </sup>T cell count had decreased, although not to the normal levels (<it>p </it>< 0.05). Both the CD8/HLADR<sup>+ </sup>and CD8/CD38<sup>+ </sup>T cell counts had gradually approached those of the control group (<it>p </it>> 0.05). In the ineffective HAART group, the CD8/CD38<sup>+ </sup>T cell count had not decreased significantly, and CD8/HLADR<sup>+ </sup>T cell count gradually decreased. Before treatment, IL-7 serum levels of patients were significantly higher than that in the control group (<it>p </it>< 0.01). After seven years of effective HAART, IL-7 levels had gradually decreased, but were still higher than in the control group (<it>p </it>< 0.01). The CD127 expression on CD3<sup>+ </sup>CD8<sup>+ </sup>T cells in effective HAART patients was higher than in untreated HIV+ patients (<it>p </it>< 0.05), but was lower than that in the control group (<it>p </it>< 0.05). CD127 expression on CD3<sup>+ </sup>CD4+ T cells was not significantly different among the control group, untreated HIV+ patients and effective HAART group.</p> <p>Conclusion</p> <p><it>After seven years of </it>effective HAART, the quantity and capacity of T cell subsets and IL-7 in HIV-1-infected patients had been partially restored, and the abnormal immune activation has significantly diminished.</p> |
topic |
HIV-1 highly active antiretroviral therapy (HAART) T cell subsets IL-7 |
url |
http://www.eurjmedres.com/content/16/11/473 |
work_keys_str_mv |
AT shouc studyoftcellsubsetsandil7proteinexpressioninhiv1infectedpatientsafter7yearshaart AT wengn studyoftcellsubsetsandil7proteinexpressioninhiv1infectedpatientsafter7yearshaart AT jiny studyoftcellsubsetsandil7proteinexpressioninhiv1infectedpatientsafter7yearshaart AT fengl studyoftcellsubsetsandil7proteinexpressioninhiv1infectedpatientsafter7yearshaart AT jinc studyoftcellsubsetsandil7proteinexpressioninhiv1infectedpatientsafter7yearshaart AT hoextermanns studyoftcellsubsetsandil7proteinexpressioninhiv1infectedpatientsafter7yearshaart AT potthoffa studyoftcellsubsetsandil7proteinexpressioninhiv1infectedpatientsafter7yearshaart AT skaletzrorowskia studyoftcellsubsetsandil7proteinexpressioninhiv1infectedpatientsafter7yearshaart AT brockmeyernh studyoftcellsubsetsandil7proteinexpressioninhiv1infectedpatientsafter7yearshaart AT wun studyoftcellsubsetsandil7proteinexpressioninhiv1infectedpatientsafter7yearshaart |
_version_ |
1716809198177615872 |