Description of microsporidia in simulids: molecular and morphological characterization of microsporidia in the larvae of Simulium pertinax Kollar (Diptera: Simuliidae)
Introduction Microsporidia constitute the most common black fly pathogens, although the species' diversity, seasonal occurrence and transmission mechanisms remain poorly understood. Infections by this agent are often chronic and non-lethal, but they can cause reduced fecundity and decreased lon...
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Sociedade Brasileira de Medicina Tropical (SBMT)
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doaj-3d8edc1d222e4e7291fa44b6648923952020-11-24T22:23:44ZengSociedade Brasileira de Medicina Tropical (SBMT)Revista da Sociedade Brasileira de Medicina Tropical1678-98492014-10-0147562463110.1590/0037-8682-0165-2014S0037-86822014000500624Description of microsporidia in simulids: molecular and morphological characterization of microsporidia in the larvae of Simulium pertinax Kollar (Diptera: Simuliidae)Isabel Maria Vicente Guedes de CarvalhoArtur Trancoso Lopo de QueirozRosiane Brito de MoraesHelio Benites GilRafael AlvesAndréa de Barros Pinto VivianiJames John BecnelCarlos José Pereira da Cunha de Araujo-CoutinhoIntroduction Microsporidia constitute the most common black fly pathogens, although the species' diversity, seasonal occurrence and transmission mechanisms remain poorly understood. Infections by this agent are often chronic and non-lethal, but they can cause reduced fecundity and decreased longevity. The objective of this study was to identify microsporidia infecting Simulium (Chirostilbia) pertinax (Kollar, 1832) larvae from Caraguatatuba, State of São Paulo, Brazil, by molecular and morphological characterization. Methods Larvae were collected at a single point in a stream in a rural area of the city and were kept under artificial aeration until analysis. Polydispyrenia spp. infection was characterized by the presence of at least 32 mononuclear spores measuring 6.9 ± 1.0 × 5.0 ± 0.7µm in persistent sporophorous vesicles. Similarly, Amblyospora spp. were characterized by the presence of eight uninucleate spores measuring 4.5 × 3.5µm in sporophorous vesicles. Results The molecular analysis confirmed the presence of microsporidian DNA in the 8 samples (prevalence of 0.51%). Six samples (Brazilian larvae) were related to Polydispyrenia simulii and Caudospora palustris reference sequences but in separate clusters. One sample was clustered with Amblyospora spp. Edhazardia aedis was the positive control taxon. Conclusions Samples identified as Polydispyrenia spp. and Amblyospora spp. were grouped with P. simulii and Amblyospora spp., respectively, corroborating previous results. However, the 16S gene tree showed a considerable distance between the black fly-infecting Amblyospora spp. and the mosquito-infecting spp. This distance suggests that these two groups are not congeneric. Additional genomic region evaluation is necessary to obtain a coherent phylogeny for this group.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822014000500624&lng=en&tlng=enMicrosporidaeAmblyospora sppPolydispyrenia sppPhylogenetic analysis |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Isabel Maria Vicente Guedes de Carvalho Artur Trancoso Lopo de Queiroz Rosiane Brito de Moraes Helio Benites Gil Rafael Alves Andréa de Barros Pinto Viviani James John Becnel Carlos José Pereira da Cunha de Araujo-Coutinho |
spellingShingle |
Isabel Maria Vicente Guedes de Carvalho Artur Trancoso Lopo de Queiroz Rosiane Brito de Moraes Helio Benites Gil Rafael Alves Andréa de Barros Pinto Viviani James John Becnel Carlos José Pereira da Cunha de Araujo-Coutinho Description of microsporidia in simulids: molecular and morphological characterization of microsporidia in the larvae of Simulium pertinax Kollar (Diptera: Simuliidae) Revista da Sociedade Brasileira de Medicina Tropical Microsporidae Amblyospora spp Polydispyrenia spp Phylogenetic analysis |
author_facet |
Isabel Maria Vicente Guedes de Carvalho Artur Trancoso Lopo de Queiroz Rosiane Brito de Moraes Helio Benites Gil Rafael Alves Andréa de Barros Pinto Viviani James John Becnel Carlos José Pereira da Cunha de Araujo-Coutinho |
author_sort |
Isabel Maria Vicente Guedes de Carvalho |
title |
Description of microsporidia in simulids: molecular and morphological characterization of microsporidia in the larvae of Simulium pertinax Kollar (Diptera: Simuliidae) |
title_short |
Description of microsporidia in simulids: molecular and morphological characterization of microsporidia in the larvae of Simulium pertinax Kollar (Diptera: Simuliidae) |
title_full |
Description of microsporidia in simulids: molecular and morphological characterization of microsporidia in the larvae of Simulium pertinax Kollar (Diptera: Simuliidae) |
title_fullStr |
Description of microsporidia in simulids: molecular and morphological characterization of microsporidia in the larvae of Simulium pertinax Kollar (Diptera: Simuliidae) |
title_full_unstemmed |
Description of microsporidia in simulids: molecular and morphological characterization of microsporidia in the larvae of Simulium pertinax Kollar (Diptera: Simuliidae) |
title_sort |
description of microsporidia in simulids: molecular and morphological characterization of microsporidia in the larvae of simulium pertinax kollar (diptera: simuliidae) |
publisher |
Sociedade Brasileira de Medicina Tropical (SBMT) |
series |
Revista da Sociedade Brasileira de Medicina Tropical |
issn |
1678-9849 |
publishDate |
2014-10-01 |
description |
Introduction Microsporidia constitute the most common black fly pathogens, although the species' diversity, seasonal occurrence and transmission mechanisms remain poorly understood. Infections by this agent are often chronic and non-lethal, but they can cause reduced fecundity and decreased longevity. The objective of this study was to identify microsporidia infecting Simulium (Chirostilbia) pertinax (Kollar, 1832) larvae from Caraguatatuba, State of São Paulo, Brazil, by molecular and morphological characterization. Methods Larvae were collected at a single point in a stream in a rural area of the city and were kept under artificial aeration until analysis. Polydispyrenia spp. infection was characterized by the presence of at least 32 mononuclear spores measuring 6.9 ± 1.0 × 5.0 ± 0.7µm in persistent sporophorous vesicles. Similarly, Amblyospora spp. were characterized by the presence of eight uninucleate spores measuring 4.5 × 3.5µm in sporophorous vesicles. Results The molecular analysis confirmed the presence of microsporidian DNA in the 8 samples (prevalence of 0.51%). Six samples (Brazilian larvae) were related to Polydispyrenia simulii and Caudospora palustris reference sequences but in separate clusters. One sample was clustered with Amblyospora spp. Edhazardia aedis was the positive control taxon. Conclusions Samples identified as Polydispyrenia spp. and Amblyospora spp. were grouped with P. simulii and Amblyospora spp., respectively, corroborating previous results. However, the 16S gene tree showed a considerable distance between the black fly-infecting Amblyospora spp. and the mosquito-infecting spp. This distance suggests that these two groups are not congeneric. Additional genomic region evaluation is necessary to obtain a coherent phylogeny for this group. |
topic |
Microsporidae Amblyospora spp Polydispyrenia spp Phylogenetic analysis |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822014000500624&lng=en&tlng=en |
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