Study on the effect of on ulcerative colitis in rats induced by 2,4,6-trinitrobenzene sulfonic acid
Ulcerative colitis (UC) is a chronic inflammatory disease of intestinal tract, and Periplaneta americana has been found to be effective in the treatment for UC. The purpose of the study was to investigate the therapeutic effect of Periplaneta americana extract Ento-A on UC in rats induced by 2,4,6-t...
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2020-08-01
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Series: | European Journal of Inflammation |
Online Access: | https://doi.org/10.1177/2058739220942629 |
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doaj-3d94d13c950541c98103a09159887e002020-11-25T03:14:59ZengSAGE PublishingEuropean Journal of Inflammation2058-73922020-08-011810.1177/2058739220942629Study on the effect of on ulcerative colitis in rats induced by 2,4,6-trinitrobenzene sulfonic acidYi-Hao Che0Zhi-Bin Yang1Han-Chao Zhang2Xiu-Mei Wu3Min-Zhe Sun4Miao Tang5Peng-Chuan Wang6Fu-Neng Geng7Ping Wan8Heng Liu9Miao He10Yu Zhao11Cheng-Gui Zhang12National-Local Joint Engineering Research Center of Entomoceutics, Dali University, Dali, Yunnan, ChinaYunnan Provincial 2011 Collaborative Innovation Center for Entomoceutics, Dali University, Dali, Yunnan, ChinaNational-Local Joint Engineering Research Center of Entomoceutics, Dali University, Dali, Yunnan, ChinaYunnan Provincial 2011 Collaborative Innovation Center for Entomoceutics, Dali University, Dali, Yunnan, ChinaNational-Local Joint Engineering Research Center of Entomoceutics, Dali University, Dali, Yunnan, ChinaNational-Local Joint Engineering Research Center of Entomoceutics, Dali University, Dali, Yunnan, ChinaNational-Local Joint Engineering Research Center of Entomoceutics, Dali University, Dali, Yunnan, ChinaGood Doctor Pharmaceutical Group, Chengdu, Sichuan, ChinaDepartment of Gastroenterology, The First People’s Hospital of Yunnan Province, Kunming, Yunnan, ChinaYunnan Provincial 2011 Collaborative Innovation Center for Entomoceutics, Dali University, Dali, Yunnan, ChinaYunnan Provincial 2011 Collaborative Innovation Center for Entomoceutics, Dali University, Dali, Yunnan, ChinaYunnan Provincial 2011 Collaborative Innovation Center for Entomoceutics, Dali University, Dali, Yunnan, ChinaYunnan Provincial 2011 Collaborative Innovation Center for Entomoceutics, Dali University, Dali, Yunnan, ChinaUlcerative colitis (UC) is a chronic inflammatory disease of intestinal tract, and Periplaneta americana has been found to be effective in the treatment for UC. The purpose of the study was to investigate the therapeutic effect of Periplaneta americana extract Ento-A on UC in rats induced by 2,4,6-trinitrobenzene sulfonic acid (TNBS) and to explore its mechanism. The Sprague-Dawley (SD) rats were randomly divided into normal control group; TNBS-treated group; sulfasalazine (SASP) treated group; Ento-A low- (50 mg/kg), medium- (100 mg/kg), and high-dose (200 mg/kg) groups, respectively. The UC model of rats was induced via TNBS. Disease activity index (DAI) was used to evaluate the severity of UC in rats. The macroscopic and microscopic damages of colon were accessed by colon mucosa damage index (CMDI) and histopathological score (HS), respectively. The levels of interleukin-4 (IL-4), interleukin-17 (IL-17), tumor necrosis factor-α (TNF-α), and interferon-γ (IFN-γ) in serum and the contents of myeloperoxidase (MPO), transforming growth factor-β1 (TGF-β1), and epidermal growth factor (EGF) in colonic mucosa were measured by enzyme-linked immunosorbent assay (ELISA). Compared with the normal control group, the TNBS-treated group showed increase in DAI, CMDI, HS, IL-17, TNF-α, IFN-γ as well as MPO and decrease in the levels of IL-4, EGF, and TGF-β1. However, Ento-A-administrated groups reversed the changes in the DAI, CMDI, HS, and the cytokines caused by TNBS. The study indicates that Periplaneta americana extract Ento-A can effectively alleviate the inflammation in TNBS-induced UC of rats, and the mechanism of that may be related to restoring the balance of T helper 1 (Th1)/Th2/Th17/T regulatory (Treg) cytokines.https://doi.org/10.1177/2058739220942629 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yi-Hao Che Zhi-Bin Yang Han-Chao Zhang Xiu-Mei Wu Min-Zhe Sun Miao Tang Peng-Chuan Wang Fu-Neng Geng Ping Wan Heng Liu Miao He Yu Zhao Cheng-Gui Zhang |
spellingShingle |
Yi-Hao Che Zhi-Bin Yang Han-Chao Zhang Xiu-Mei Wu Min-Zhe Sun Miao Tang Peng-Chuan Wang Fu-Neng Geng Ping Wan Heng Liu Miao He Yu Zhao Cheng-Gui Zhang Study on the effect of on ulcerative colitis in rats induced by 2,4,6-trinitrobenzene sulfonic acid European Journal of Inflammation |
author_facet |
Yi-Hao Che Zhi-Bin Yang Han-Chao Zhang Xiu-Mei Wu Min-Zhe Sun Miao Tang Peng-Chuan Wang Fu-Neng Geng Ping Wan Heng Liu Miao He Yu Zhao Cheng-Gui Zhang |
author_sort |
Yi-Hao Che |
title |
Study on the effect of on ulcerative colitis in rats induced by 2,4,6-trinitrobenzene sulfonic acid |
title_short |
Study on the effect of on ulcerative colitis in rats induced by 2,4,6-trinitrobenzene sulfonic acid |
title_full |
Study on the effect of on ulcerative colitis in rats induced by 2,4,6-trinitrobenzene sulfonic acid |
title_fullStr |
Study on the effect of on ulcerative colitis in rats induced by 2,4,6-trinitrobenzene sulfonic acid |
title_full_unstemmed |
Study on the effect of on ulcerative colitis in rats induced by 2,4,6-trinitrobenzene sulfonic acid |
title_sort |
study on the effect of on ulcerative colitis in rats induced by 2,4,6-trinitrobenzene sulfonic acid |
publisher |
SAGE Publishing |
series |
European Journal of Inflammation |
issn |
2058-7392 |
publishDate |
2020-08-01 |
description |
Ulcerative colitis (UC) is a chronic inflammatory disease of intestinal tract, and Periplaneta americana has been found to be effective in the treatment for UC. The purpose of the study was to investigate the therapeutic effect of Periplaneta americana extract Ento-A on UC in rats induced by 2,4,6-trinitrobenzene sulfonic acid (TNBS) and to explore its mechanism. The Sprague-Dawley (SD) rats were randomly divided into normal control group; TNBS-treated group; sulfasalazine (SASP) treated group; Ento-A low- (50 mg/kg), medium- (100 mg/kg), and high-dose (200 mg/kg) groups, respectively. The UC model of rats was induced via TNBS. Disease activity index (DAI) was used to evaluate the severity of UC in rats. The macroscopic and microscopic damages of colon were accessed by colon mucosa damage index (CMDI) and histopathological score (HS), respectively. The levels of interleukin-4 (IL-4), interleukin-17 (IL-17), tumor necrosis factor-α (TNF-α), and interferon-γ (IFN-γ) in serum and the contents of myeloperoxidase (MPO), transforming growth factor-β1 (TGF-β1), and epidermal growth factor (EGF) in colonic mucosa were measured by enzyme-linked immunosorbent assay (ELISA). Compared with the normal control group, the TNBS-treated group showed increase in DAI, CMDI, HS, IL-17, TNF-α, IFN-γ as well as MPO and decrease in the levels of IL-4, EGF, and TGF-β1. However, Ento-A-administrated groups reversed the changes in the DAI, CMDI, HS, and the cytokines caused by TNBS. The study indicates that Periplaneta americana extract Ento-A can effectively alleviate the inflammation in TNBS-induced UC of rats, and the mechanism of that may be related to restoring the balance of T helper 1 (Th1)/Th2/Th17/T regulatory (Treg) cytokines. |
url |
https://doi.org/10.1177/2058739220942629 |
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