A single amino acid polymorphism in the glycosyltransferase CpsK defines four Streptococcus suis serotypes
Abstract The capsular polysaccharide (CPS) is the major virulence factor of the emerging zoonotic pathogen Streptococcus suis. CPS differences are also the basis for serological differentiation of the species into 29 serotypes. Serotypes 2 and 1/2, which possess identical gene content in their cps l...
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2017-06-01
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Online Access: | https://doi.org/10.1038/s41598-017-04403-3 |
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doaj-3d9c511fd06949b3a5702c341d1b33382020-12-08T00:24:15ZengNature Publishing GroupScientific Reports2045-23222017-06-017111310.1038/s41598-017-04403-3A single amino acid polymorphism in the glycosyltransferase CpsK defines four Streptococcus suis serotypesDavid Roy0Taryn B. T. Athey1Jean-Philippe Auger2Guillaume Goyette-Desjardins3Marie-Rose Van Calsteren4Daisuke Takamatsu5Masatoshi Okura6Sarah Teatero7Martín Alcorlo8Juan A. Hermoso9Mariela Segura10Marcelo Gottschalk11Nahuel Fittipaldi12Swine and Poultry Infectious Diseases Research Centre, Faculty of Veterinary Medicine, University of MontrealPublic Health Ontario, Toronto LaboratorySwine and Poultry Infectious Diseases Research Centre, Faculty of Veterinary Medicine, University of MontrealSwine and Poultry Infectious Diseases Research Centre, Faculty of Veterinary Medicine, University of MontrealSwine and Poultry Infectious Diseases Research Centre, Faculty of Veterinary Medicine, University of MontrealDivision of Bacterial and Parasitic Diseases, National Institute of Animal Health, National Agriculture and Food Research OrganizationDivision of Bacterial and Parasitic Diseases, National Institute of Animal Health, National Agriculture and Food Research OrganizationPublic Health Ontario, Toronto LaboratoryDepartment of Crystallography and Structural Biology, Instituto de Química-Física “Rocasolano”, Consejo Superior de Investigaciones CientíficasDepartment of Crystallography and Structural Biology, Instituto de Química-Física “Rocasolano”, Consejo Superior de Investigaciones CientíficasSwine and Poultry Infectious Diseases Research Centre, Faculty of Veterinary Medicine, University of MontrealSwine and Poultry Infectious Diseases Research Centre, Faculty of Veterinary Medicine, University of MontrealPublic Health Ontario, Toronto LaboratoryAbstract The capsular polysaccharide (CPS) is the major virulence factor of the emerging zoonotic pathogen Streptococcus suis. CPS differences are also the basis for serological differentiation of the species into 29 serotypes. Serotypes 2 and 1/2, which possess identical gene content in their cps loci, express CPSs that differ only by substitution of galactose (Gal) by N-acetylgalactosamine (GalNAc) in the CPS side chain. The same sugar substitution differentiates the CPS of serotypes 14 and 1, whose cps loci are also identical in gene content. Here, using mutagenesis, CPS structural analysis, and protein structure modeling, we report that a single amino acid polymorphism in the glycosyltransferase CpsK defines the enzyme substrate predilection for Gal or GalNAc and therefore determines CPS composition, structure, and strain serotype. We also show that the different CPS structures have similar antiphagocytic properties and that serotype switching has limited impact on the virulence of S. suis.https://doi.org/10.1038/s41598-017-04403-3 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
David Roy Taryn B. T. Athey Jean-Philippe Auger Guillaume Goyette-Desjardins Marie-Rose Van Calsteren Daisuke Takamatsu Masatoshi Okura Sarah Teatero Martín Alcorlo Juan A. Hermoso Mariela Segura Marcelo Gottschalk Nahuel Fittipaldi |
spellingShingle |
David Roy Taryn B. T. Athey Jean-Philippe Auger Guillaume Goyette-Desjardins Marie-Rose Van Calsteren Daisuke Takamatsu Masatoshi Okura Sarah Teatero Martín Alcorlo Juan A. Hermoso Mariela Segura Marcelo Gottschalk Nahuel Fittipaldi A single amino acid polymorphism in the glycosyltransferase CpsK defines four Streptococcus suis serotypes Scientific Reports |
author_facet |
David Roy Taryn B. T. Athey Jean-Philippe Auger Guillaume Goyette-Desjardins Marie-Rose Van Calsteren Daisuke Takamatsu Masatoshi Okura Sarah Teatero Martín Alcorlo Juan A. Hermoso Mariela Segura Marcelo Gottschalk Nahuel Fittipaldi |
author_sort |
David Roy |
title |
A single amino acid polymorphism in the glycosyltransferase CpsK defines four Streptococcus suis serotypes |
title_short |
A single amino acid polymorphism in the glycosyltransferase CpsK defines four Streptococcus suis serotypes |
title_full |
A single amino acid polymorphism in the glycosyltransferase CpsK defines four Streptococcus suis serotypes |
title_fullStr |
A single amino acid polymorphism in the glycosyltransferase CpsK defines four Streptococcus suis serotypes |
title_full_unstemmed |
A single amino acid polymorphism in the glycosyltransferase CpsK defines four Streptococcus suis serotypes |
title_sort |
single amino acid polymorphism in the glycosyltransferase cpsk defines four streptococcus suis serotypes |
publisher |
Nature Publishing Group |
series |
Scientific Reports |
issn |
2045-2322 |
publishDate |
2017-06-01 |
description |
Abstract The capsular polysaccharide (CPS) is the major virulence factor of the emerging zoonotic pathogen Streptococcus suis. CPS differences are also the basis for serological differentiation of the species into 29 serotypes. Serotypes 2 and 1/2, which possess identical gene content in their cps loci, express CPSs that differ only by substitution of galactose (Gal) by N-acetylgalactosamine (GalNAc) in the CPS side chain. The same sugar substitution differentiates the CPS of serotypes 14 and 1, whose cps loci are also identical in gene content. Here, using mutagenesis, CPS structural analysis, and protein structure modeling, we report that a single amino acid polymorphism in the glycosyltransferase CpsK defines the enzyme substrate predilection for Gal or GalNAc and therefore determines CPS composition, structure, and strain serotype. We also show that the different CPS structures have similar antiphagocytic properties and that serotype switching has limited impact on the virulence of S. suis. |
url |
https://doi.org/10.1038/s41598-017-04403-3 |
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