Clarifying CLARITY: quantitative optimization of the diffusion based delipidation protocol for genetically labelled tissue

Clarifying CLARITY: quantitative optimization of the diffusion based delipidation protocol for genetically labelled tissue

Tissue clarification has been recently proposed to allow deep tissue imaging without light scattering. The clarification parameters are somewhat arbitrary and dependent on tissue type, source and dimension: every laboratory has its own protocol, but a quantitative approach to determine the optimum c...

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Bibliographic Details
Main Authors: Chiara eMagliaro, Alejandro Luis Callara, Giorgio eMattei, Marco eMorcinelli, Maria Cristina eViaggi, Francesca eVaglini, Arti eAhluwalia
Format: Article
Language:English
Published: Frontiers Media S.A. 2016-04-01
Series:Frontiers in Neuroscience
Subjects:
GFP
image processing
CLARITY
Mouse brain slices
quantitative protocol optimization
Online Access:http://journal.frontiersin.org/Journal/10.3389/fnins.2016.00179/full

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