Comparative Analysis of Fecal Microbiota Composition Between Rheumatoid Arthritis and Osteoarthritis Patients
The aim of this study was to investigate differences between the gut microbiota composition in patients with rheumatoid arthritis (RA) and those with osteoarthritis (OA). Stool samples from nine RA patients and nine OA patients were collected, and DNA was extracted. The gut microbiome was assessed u...
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doaj-3da28167ca434cb1a82d8e9d9b760c2e2020-11-25T00:55:40ZengMDPI AGGenes2073-44252019-09-01101074810.3390/genes10100748genes10100748Comparative Analysis of Fecal Microbiota Composition Between Rheumatoid Arthritis and Osteoarthritis PatientsJin-Young Lee0Mohamed Mannaa1Yunkyung Kim2Jehun Kim3Geun-Tae Kim4Young-Su Seo5Department of Internal Medicine, Kosin University College of Medicine, Busan 49104, KoreaDepartment of Integrated Biological Science, Pusan National University, Busan 46241, KoreaDepartment of Internal Medicine, Kosin University College of Medicine, Busan 49104, KoreaDepartment of Internal Medicine, Kosin University College of Medicine, Busan 49104, KoreaDepartment of Internal Medicine, Kosin University College of Medicine, Busan 49104, KoreaDepartment of Integrated Biological Science, Pusan National University, Busan 46241, KoreaThe aim of this study was to investigate differences between the gut microbiota composition in patients with rheumatoid arthritis (RA) and those with osteoarthritis (OA). Stool samples from nine RA patients and nine OA patients were collected, and DNA was extracted. The gut microbiome was assessed using 16S rRNA gene amplicon sequencing. The structures and differences in the gut microbiome between RA and OA were analyzed. The analysis of diversity revealed no differences in the complexity of samples. The RA group had a lower Bacteroidetes: Firmicutes ratio than did the OA group. Lactobacilli and <i>Prevotella</i>, particularly <i>Prevotella copri</i>, were more abundant in the RA than in the OA group, although these differences were not statistically significant. The relative abundance of Bacteroides and <i>Bifidobacterium</i> was lower in the RA group. At the species level, the abundance of certain bacterial species was significantly lower in the RA group, such as <i>Fusicatenibacter saccharivorans, Dialister invisus, Clostridium leptum, Ruthenibacterium lactatiformans, Anaerotruncus colihominis, Bacteroides faecichinchillae, Harryflintia acetispora, Bacteroides acidifaciens, and Christensenella minuta</i>. The microbial properties of the gut differed between RA and OA patients, and the RA dysbiosis revealed results similar to those of other autoimmune diseases, suggesting that a specific gut microbiota pattern is related to autoimmunity.https://www.mdpi.com/2073-4425/10/10/748rheumatoid arthritisosteoarthritisgut microbiota |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jin-Young Lee Mohamed Mannaa Yunkyung Kim Jehun Kim Geun-Tae Kim Young-Su Seo |
spellingShingle |
Jin-Young Lee Mohamed Mannaa Yunkyung Kim Jehun Kim Geun-Tae Kim Young-Su Seo Comparative Analysis of Fecal Microbiota Composition Between Rheumatoid Arthritis and Osteoarthritis Patients Genes rheumatoid arthritis osteoarthritis gut microbiota |
author_facet |
Jin-Young Lee Mohamed Mannaa Yunkyung Kim Jehun Kim Geun-Tae Kim Young-Su Seo |
author_sort |
Jin-Young Lee |
title |
Comparative Analysis of Fecal Microbiota Composition Between Rheumatoid Arthritis and Osteoarthritis Patients |
title_short |
Comparative Analysis of Fecal Microbiota Composition Between Rheumatoid Arthritis and Osteoarthritis Patients |
title_full |
Comparative Analysis of Fecal Microbiota Composition Between Rheumatoid Arthritis and Osteoarthritis Patients |
title_fullStr |
Comparative Analysis of Fecal Microbiota Composition Between Rheumatoid Arthritis and Osteoarthritis Patients |
title_full_unstemmed |
Comparative Analysis of Fecal Microbiota Composition Between Rheumatoid Arthritis and Osteoarthritis Patients |
title_sort |
comparative analysis of fecal microbiota composition between rheumatoid arthritis and osteoarthritis patients |
publisher |
MDPI AG |
series |
Genes |
issn |
2073-4425 |
publishDate |
2019-09-01 |
description |
The aim of this study was to investigate differences between the gut microbiota composition in patients with rheumatoid arthritis (RA) and those with osteoarthritis (OA). Stool samples from nine RA patients and nine OA patients were collected, and DNA was extracted. The gut microbiome was assessed using 16S rRNA gene amplicon sequencing. The structures and differences in the gut microbiome between RA and OA were analyzed. The analysis of diversity revealed no differences in the complexity of samples. The RA group had a lower Bacteroidetes: Firmicutes ratio than did the OA group. Lactobacilli and <i>Prevotella</i>, particularly <i>Prevotella copri</i>, were more abundant in the RA than in the OA group, although these differences were not statistically significant. The relative abundance of Bacteroides and <i>Bifidobacterium</i> was lower in the RA group. At the species level, the abundance of certain bacterial species was significantly lower in the RA group, such as <i>Fusicatenibacter saccharivorans, Dialister invisus, Clostridium leptum, Ruthenibacterium lactatiformans, Anaerotruncus colihominis, Bacteroides faecichinchillae, Harryflintia acetispora, Bacteroides acidifaciens, and Christensenella minuta</i>. The microbial properties of the gut differed between RA and OA patients, and the RA dysbiosis revealed results similar to those of other autoimmune diseases, suggesting that a specific gut microbiota pattern is related to autoimmunity. |
topic |
rheumatoid arthritis osteoarthritis gut microbiota |
url |
https://www.mdpi.com/2073-4425/10/10/748 |
work_keys_str_mv |
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