DNA barcoding coupled with high resolution melting for rapid identification of Ardisia gigantifolia and its toxic adulterants
Ardisia gigantifolia is a medicinal plant; its herbal medicinal product has played important roles in the ways of life of people in China. However, commercial products of this herbal medicine are frequently adulterated with Rhododendron molle root and Clerodendrum cyrtophyllum stem. In fact, R. moll...
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Online Access: | http://dx.doi.org/10.1080/13102818.2021.1885993 |
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doaj-3dae9db085cf454d8b91c01b68e112a42021-06-02T08:05:30ZengTaylor & Francis GroupBiotechnology & Biotechnological Equipment1310-28181314-35302021-01-0135164164910.1080/13102818.2021.18859931885993DNA barcoding coupled with high resolution melting for rapid identification of Ardisia gigantifolia and its toxic adulterantsBo Zhao0Chao Xiong1Lan Wu2Li Xiang3Yuhua Shi4Wei Sun5Shilin Chen6Key Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine, Institute of Chinese Materia Medica, China Academy of Chinese Medical SciencesKey Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine, Institute of Chinese Materia Medica, China Academy of Chinese Medical SciencesKey Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine, Institute of Chinese Materia Medica, China Academy of Chinese Medical SciencesKey Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine, Institute of Chinese Materia Medica, China Academy of Chinese Medical SciencesKey Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine, Institute of Chinese Materia Medica, China Academy of Chinese Medical SciencesKey Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine, Institute of Chinese Materia Medica, China Academy of Chinese Medical SciencesKey Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine, Institute of Chinese Materia Medica, China Academy of Chinese Medical SciencesArdisia gigantifolia is a medicinal plant; its herbal medicinal product has played important roles in the ways of life of people in China. However, commercial products of this herbal medicine are frequently adulterated with Rhododendron molle root and Clerodendrum cyrtophyllum stem. In fact, R. molle has hepatotoxic effects which could pose health issues to the end user. In this study, we applied DNA barcoding coupled with high resolution melting (Bar-HRM) to distinguish A. gigantifolia and adulterant products. Two DNA barcode loci (ITS2 and psbA-trnH) were chosen for Bar-HRM analysis. We found that ITS2 and psbA-trnH regions contain some inter-species variability, which showed enough discrimination capability for Bar-HRM analysis. We applied the Bar-HRM method to test the method, and found that some products labelled as “A. gigantifolia” collected from local markets in China were identified to contain C. cyrtophyllum. Our study demonstrated that DNA barcoding coupled with high resolution melting can be used as a routine test to guarantee the quality of A. gigantifolia herbal products.http://dx.doi.org/10.1080/13102818.2021.1885993ardisia gigantifoliadna barcodingits2 and psba-trnhbar-hrmrapid identification |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Bo Zhao Chao Xiong Lan Wu Li Xiang Yuhua Shi Wei Sun Shilin Chen |
spellingShingle |
Bo Zhao Chao Xiong Lan Wu Li Xiang Yuhua Shi Wei Sun Shilin Chen DNA barcoding coupled with high resolution melting for rapid identification of Ardisia gigantifolia and its toxic adulterants Biotechnology & Biotechnological Equipment ardisia gigantifolia dna barcoding its2 and psba-trnh bar-hrm rapid identification |
author_facet |
Bo Zhao Chao Xiong Lan Wu Li Xiang Yuhua Shi Wei Sun Shilin Chen |
author_sort |
Bo Zhao |
title |
DNA barcoding coupled with high resolution melting for rapid identification of Ardisia gigantifolia and its toxic adulterants |
title_short |
DNA barcoding coupled with high resolution melting for rapid identification of Ardisia gigantifolia and its toxic adulterants |
title_full |
DNA barcoding coupled with high resolution melting for rapid identification of Ardisia gigantifolia and its toxic adulterants |
title_fullStr |
DNA barcoding coupled with high resolution melting for rapid identification of Ardisia gigantifolia and its toxic adulterants |
title_full_unstemmed |
DNA barcoding coupled with high resolution melting for rapid identification of Ardisia gigantifolia and its toxic adulterants |
title_sort |
dna barcoding coupled with high resolution melting for rapid identification of ardisia gigantifolia and its toxic adulterants |
publisher |
Taylor & Francis Group |
series |
Biotechnology & Biotechnological Equipment |
issn |
1310-2818 1314-3530 |
publishDate |
2021-01-01 |
description |
Ardisia gigantifolia is a medicinal plant; its herbal medicinal product has played important roles in the ways of life of people in China. However, commercial products of this herbal medicine are frequently adulterated with Rhododendron molle root and Clerodendrum cyrtophyllum stem. In fact, R. molle has hepatotoxic effects which could pose health issues to the end user. In this study, we applied DNA barcoding coupled with high resolution melting (Bar-HRM) to distinguish A. gigantifolia and adulterant products. Two DNA barcode loci (ITS2 and psbA-trnH) were chosen for Bar-HRM analysis. We found that ITS2 and psbA-trnH regions contain some inter-species variability, which showed enough discrimination capability for Bar-HRM analysis. We applied the Bar-HRM method to test the method, and found that some products labelled as “A. gigantifolia” collected from local markets in China were identified to contain C. cyrtophyllum. Our study demonstrated that DNA barcoding coupled with high resolution melting can be used as a routine test to guarantee the quality of A. gigantifolia herbal products. |
topic |
ardisia gigantifolia dna barcoding its2 and psba-trnh bar-hrm rapid identification |
url |
http://dx.doi.org/10.1080/13102818.2021.1885993 |
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