A Motor-Driven Mechanism for Cell-Length Sensing
Size homeostasis is fundamental in cell biology, but it is not clear how large cells such as neurons can assess their own size or length. We examined a role for molecular motors in intracellular length sensing. Computational simulations suggest that spatial information can be encoded by the frequen...
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doaj-3dd069deda0f46e58c881986091e936e2020-11-25T00:46:48ZengElsevierCell Reports2211-12472012-06-011660861610.1016/j.celrep.2012.05.013A Motor-Driven Mechanism for Cell-Length SensingIda Rishal0Naaman Kam1Rotem Ben-Tov Perry2Vera Shinder3Elizabeth M.C. Fisher4Giampietro Schiavo5Mike Fainzilber6Department of Biological Chemistry, Weizmann Institute of Science, 76100 Rehovot, IsraelDepartment of Biological Chemistry, Weizmann Institute of Science, 76100 Rehovot, IsraelDepartment of Biological Chemistry, Weizmann Institute of Science, 76100 Rehovot, IsraelDepartment of Chemical Research Support, Weizmann Institute of Science, 76100 Rehovot, IsraelDepartment of Neurodegenerative Disease and MRC Centre for Neuromuscular Diseases, Institute of Neurology, University College London, London WC1N 3BG, UKMolecular Neuropathobiology Laboratory, Cancer Research UK London Research Institute, Lincoln's Inn Fields Laboratories, London WC2A 3PX, UKDepartment of Biological Chemistry, Weizmann Institute of Science, 76100 Rehovot, Israel Size homeostasis is fundamental in cell biology, but it is not clear how large cells such as neurons can assess their own size or length. We examined a role for molecular motors in intracellular length sensing. Computational simulations suggest that spatial information can be encoded by the frequency of an oscillating retrograde signal arising from a composite negative feedback loop between bidirectional motor-dependent signals. The model predicts that decreasing either or both anterograde or retrograde signals should increase cell length, and this prediction was confirmed upon application of siRNAs for specific kinesin and/or dynein heavy chains in adult sensory neurons. Heterozygous dynein heavy chain 1 mutant sensory neurons also exhibited increased lengths both in vitro and during embryonic development. Moreover, similar length increases were observed in mouse embryonic fibroblasts upon partial downregulation of dynein heavy chain 1. Thus, molecular motors critically influence cell-length sensing and growth control. http://www.sciencedirect.com/science/article/pii/S2211124712001386 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ida Rishal Naaman Kam Rotem Ben-Tov Perry Vera Shinder Elizabeth M.C. Fisher Giampietro Schiavo Mike Fainzilber |
spellingShingle |
Ida Rishal Naaman Kam Rotem Ben-Tov Perry Vera Shinder Elizabeth M.C. Fisher Giampietro Schiavo Mike Fainzilber A Motor-Driven Mechanism for Cell-Length Sensing Cell Reports |
author_facet |
Ida Rishal Naaman Kam Rotem Ben-Tov Perry Vera Shinder Elizabeth M.C. Fisher Giampietro Schiavo Mike Fainzilber |
author_sort |
Ida Rishal |
title |
A Motor-Driven Mechanism for Cell-Length Sensing |
title_short |
A Motor-Driven Mechanism for Cell-Length Sensing |
title_full |
A Motor-Driven Mechanism for Cell-Length Sensing |
title_fullStr |
A Motor-Driven Mechanism for Cell-Length Sensing |
title_full_unstemmed |
A Motor-Driven Mechanism for Cell-Length Sensing |
title_sort |
motor-driven mechanism for cell-length sensing |
publisher |
Elsevier |
series |
Cell Reports |
issn |
2211-1247 |
publishDate |
2012-06-01 |
description |
Size homeostasis is fundamental in cell biology, but it is not clear how large cells such as neurons can assess their own size or length. We examined a role for molecular motors in intracellular length sensing. Computational simulations suggest that spatial information can be encoded by the frequency of an oscillating retrograde signal arising from a composite negative feedback loop between bidirectional motor-dependent signals. The model predicts that decreasing either or both anterograde or retrograde signals should increase cell length, and this prediction was confirmed upon application of siRNAs for specific kinesin and/or dynein heavy chains in adult sensory neurons. Heterozygous dynein heavy chain 1 mutant sensory neurons also exhibited increased lengths both in vitro and during embryonic development. Moreover, similar length increases were observed in mouse embryonic fibroblasts upon partial downregulation of dynein heavy chain 1. Thus, molecular motors critically influence cell-length sensing and growth control.
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url |
http://www.sciencedirect.com/science/article/pii/S2211124712001386 |
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