Radix Puerariae and Fructus Crataegi mixture inhibits renal injury in type 2 diabetes via decreasing of AKT/PI3K
Abstract Background Radix puerariae (RP) is a herbal medicines for diabetes, mainly because of anti-oxidative, insulin resistance and hypoglycemic effect. Fructus crataegi (FC) also possesses strong antioxidant activity in vitro. This study focused on the effects of herbal mixture of RP and FC (RPFC...
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doaj-3dea41fb26d64297802ba68ef6c8a6fb2020-11-25T03:22:15ZengBMCBMC Complementary and Alternative Medicine1472-68822017-09-011711910.1186/s12906-017-1945-3Radix Puerariae and Fructus Crataegi mixture inhibits renal injury in type 2 diabetes via decreasing of AKT/PI3KZhengyue Chen0Yanyan Yuan1Xinrong Zou2Mengqi Hong3Ming Zhao4Yu Zhao5Yuanping Liu6Guofu Li7Yabin Zhu8Lin Luo9Beiyan Bao10Shizhong Bu11Division of Nephrology, Ningbo Urology and Nephrology HospitalRunliang Diabetes Laboratory, Diabetes Research Center, School of Medicine, Ningbo UniversityRunliang Diabetes Laboratory, Diabetes Research Center, School of Medicine, Ningbo UniversityDivision of Nephrology, Ningbo Urology and Nephrology HospitalRunliang Diabetes Laboratory, Diabetes Research Center, School of Medicine, Ningbo UniversityDivision of Nephrology, Ningbo Urology and Nephrology HospitalDivision of Nephrology, Ningbo Urology and Nephrology HospitalDivision of Nephrology, Ningbo Urology and Nephrology HospitalSchool of Medicine, Ningbo UniversitySchool of Medicine, Ningbo UniversityDivision of Nephrology, Ningbo Urology and Nephrology HospitalRunliang Diabetes Laboratory, Diabetes Research Center, School of Medicine, Ningbo UniversityAbstract Background Radix puerariae (RP) is a herbal medicines for diabetes, mainly because of anti-oxidative, insulin resistance and hypoglycemic effect. Fructus crataegi (FC) also possesses strong antioxidant activity in vitro. This study focused on the effects of herbal mixture of RP and FC (RPFC) on renal protection through a diabetic rat model. Methods Type 2 Diabetic model was established with high fat diet followed by injecting rats a low dose of STZ (25 mg/kg body weight). Rats were randomly divided into five groups: normal, high fat diet, diabetes mellitus, high fat diet plus RPFC prevention, and RPFC prevention before diabetes mellitus. RPFC was given to rats daily by intragastric gavage. The blood bio-chemical index and renal pathological changes were examined. The later includes hematoxylin and eosin staining, periodic acid schiff staining, and Masson trichrome staining. Protein levels of were determined by Western blot and immunohistochemical staining. mRNA levels were detected by RT-PCR. Results Rats prevented with RPFC resulted in decreasing blood glucose with corresponding vehicle treated rats. Glomerulus mesangial matrix expansion, renal capsule constriction, and renal tubular epithelial cell edema were less severe following RPFC prevention. Moreover, RPFC prevention reduced protein levels of PI3K, AKT, α-SMA and collagen IV in the kidney of diabetic rats. Conclusion Combined prevention with RPFC may inhibit the PI3K/AKT pathway in the kidney, thereby prevent renal injury in diabetic rats.http://link.springer.com/article/10.1186/s12906-017-1945-3Type 2 diabetesRadix PuerariaeFructus CrataegiRenal injuryPI3K/AKT |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Zhengyue Chen Yanyan Yuan Xinrong Zou Mengqi Hong Ming Zhao Yu Zhao Yuanping Liu Guofu Li Yabin Zhu Lin Luo Beiyan Bao Shizhong Bu |
spellingShingle |
Zhengyue Chen Yanyan Yuan Xinrong Zou Mengqi Hong Ming Zhao Yu Zhao Yuanping Liu Guofu Li Yabin Zhu Lin Luo Beiyan Bao Shizhong Bu Radix Puerariae and Fructus Crataegi mixture inhibits renal injury in type 2 diabetes via decreasing of AKT/PI3K BMC Complementary and Alternative Medicine Type 2 diabetes Radix Puerariae Fructus Crataegi Renal injury PI3K/AKT |
author_facet |
Zhengyue Chen Yanyan Yuan Xinrong Zou Mengqi Hong Ming Zhao Yu Zhao Yuanping Liu Guofu Li Yabin Zhu Lin Luo Beiyan Bao Shizhong Bu |
author_sort |
Zhengyue Chen |
title |
Radix Puerariae and Fructus Crataegi mixture inhibits renal injury in type 2 diabetes via decreasing of AKT/PI3K |
title_short |
Radix Puerariae and Fructus Crataegi mixture inhibits renal injury in type 2 diabetes via decreasing of AKT/PI3K |
title_full |
Radix Puerariae and Fructus Crataegi mixture inhibits renal injury in type 2 diabetes via decreasing of AKT/PI3K |
title_fullStr |
Radix Puerariae and Fructus Crataegi mixture inhibits renal injury in type 2 diabetes via decreasing of AKT/PI3K |
title_full_unstemmed |
Radix Puerariae and Fructus Crataegi mixture inhibits renal injury in type 2 diabetes via decreasing of AKT/PI3K |
title_sort |
radix puerariae and fructus crataegi mixture inhibits renal injury in type 2 diabetes via decreasing of akt/pi3k |
publisher |
BMC |
series |
BMC Complementary and Alternative Medicine |
issn |
1472-6882 |
publishDate |
2017-09-01 |
description |
Abstract Background Radix puerariae (RP) is a herbal medicines for diabetes, mainly because of anti-oxidative, insulin resistance and hypoglycemic effect. Fructus crataegi (FC) also possesses strong antioxidant activity in vitro. This study focused on the effects of herbal mixture of RP and FC (RPFC) on renal protection through a diabetic rat model. Methods Type 2 Diabetic model was established with high fat diet followed by injecting rats a low dose of STZ (25 mg/kg body weight). Rats were randomly divided into five groups: normal, high fat diet, diabetes mellitus, high fat diet plus RPFC prevention, and RPFC prevention before diabetes mellitus. RPFC was given to rats daily by intragastric gavage. The blood bio-chemical index and renal pathological changes were examined. The later includes hematoxylin and eosin staining, periodic acid schiff staining, and Masson trichrome staining. Protein levels of were determined by Western blot and immunohistochemical staining. mRNA levels were detected by RT-PCR. Results Rats prevented with RPFC resulted in decreasing blood glucose with corresponding vehicle treated rats. Glomerulus mesangial matrix expansion, renal capsule constriction, and renal tubular epithelial cell edema were less severe following RPFC prevention. Moreover, RPFC prevention reduced protein levels of PI3K, AKT, α-SMA and collagen IV in the kidney of diabetic rats. Conclusion Combined prevention with RPFC may inhibit the PI3K/AKT pathway in the kidney, thereby prevent renal injury in diabetic rats. |
topic |
Type 2 diabetes Radix Puerariae Fructus Crataegi Renal injury PI3K/AKT |
url |
http://link.springer.com/article/10.1186/s12906-017-1945-3 |
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