Circular RNA hsa_circ_0005909 modulates osteosarcoma progression via the miR-936/HMGB1 axis
Abstract Background Osteosarcoma (OS) is the most common bone malignant tumor in children, youth, and adolescents. Circular RNA hsa_circ_0005909 (circ_0005909) is involved in the progression of OS. Nevertheless, there are few reports on the role and mechanism of circ_0005909 in OS. Methods Quantitat...
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doaj-3dedd494408643608e546c698174fd482020-11-25T02:52:29ZengBMCCancer Cell International1475-28672020-07-0120111210.1186/s12935-020-01399-1Circular RNA hsa_circ_0005909 modulates osteosarcoma progression via the miR-936/HMGB1 axisShuai Ding0Guangquan Zhang1Yanzheng Gao2Shulian Chen3Chen Cao4Department of Spine and Spinal Cord, Henan Provincial People’s Hospital, People’s Hospital of Zhengzhou University, People’s Hospital of Henan UniversityDepartment of Spine and Spinal Cord, Henan Provincial People’s Hospital, People’s Hospital of Zhengzhou University, People’s Hospital of Henan UniversityDepartment of Spine and Spinal Cord, Henan Provincial People’s Hospital, People’s Hospital of Zhengzhou University, People’s Hospital of Henan UniversityDepartment of Spine and Spinal Cord, Henan Provincial People’s Hospital, People’s Hospital of Zhengzhou University, People’s Hospital of Henan UniversityDepartment of Spine and Spinal Cord, Henan Provincial People’s Hospital, People’s Hospital of Zhengzhou University, People’s Hospital of Henan UniversityAbstract Background Osteosarcoma (OS) is the most common bone malignant tumor in children, youth, and adolescents. Circular RNA hsa_circ_0005909 (circ_0005909) is involved in the progression of OS. Nevertheless, there are few reports on the role and mechanism of circ_0005909 in OS. Methods Quantitative real-time polymerase chain reaction (qRT-PCR) was executed to examine the expression of circ_0005909, miR-936, and High Mobility Group Box 1 (HMGB1) mRNA in OS tissues and cells. Cell viability, colony formation, migration, and invasion were evaluated by Cell Counting Kit-8 (CCK-8), cell colony formation, or transwell assays. Cell epithelial-mesenchymal transition (EMT) and HMGB1 protein levels were assessed through western blot analysis. The role of circ_0005909 on tumor growth in vivo was verified by xenograft assay. The relationship between circ_0005909 or HMGB1 and miR-936 was confirmed with the dual-luciferase reporter or RNA pull-down assays. Results Circ_0005909 level was upregulated in OS tissues and cells. OS patients with high circ_0005909 expression had a lower survival rate. Circ_0005909 inhibition reduced tumor growth in vivo and constrained cell viability, colony formation, migration, invasion, and EMT of OS cells in vitro. Furthermore, circ_0005909 served as a sponge for miR-936 and the repressive impacts of circ_0005909 silencing on malignant behaviors of OS cells were abolished by miR-936 inhibitors. Also, HMGB1 acted as a target for miR-936 and was modulated by circ_0005909 via miR-936. Additionally, HMGB1 overexpression restored the inhibitory influence on the malignant behaviors of OS cells mediated by circ_0005909 inhibition. Conclusions Circ_0005909 inhibition impeded the progression of OS via downregulating HMGB1 via sponging miR-936.http://link.springer.com/article/10.1186/s12935-020-01399-1OScirc_0005909miR-936HMGB1OS cell lines |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Shuai Ding Guangquan Zhang Yanzheng Gao Shulian Chen Chen Cao |
spellingShingle |
Shuai Ding Guangquan Zhang Yanzheng Gao Shulian Chen Chen Cao Circular RNA hsa_circ_0005909 modulates osteosarcoma progression via the miR-936/HMGB1 axis Cancer Cell International OS circ_0005909 miR-936 HMGB1 OS cell lines |
author_facet |
Shuai Ding Guangquan Zhang Yanzheng Gao Shulian Chen Chen Cao |
author_sort |
Shuai Ding |
title |
Circular RNA hsa_circ_0005909 modulates osteosarcoma progression via the miR-936/HMGB1 axis |
title_short |
Circular RNA hsa_circ_0005909 modulates osteosarcoma progression via the miR-936/HMGB1 axis |
title_full |
Circular RNA hsa_circ_0005909 modulates osteosarcoma progression via the miR-936/HMGB1 axis |
title_fullStr |
Circular RNA hsa_circ_0005909 modulates osteosarcoma progression via the miR-936/HMGB1 axis |
title_full_unstemmed |
Circular RNA hsa_circ_0005909 modulates osteosarcoma progression via the miR-936/HMGB1 axis |
title_sort |
circular rna hsa_circ_0005909 modulates osteosarcoma progression via the mir-936/hmgb1 axis |
publisher |
BMC |
series |
Cancer Cell International |
issn |
1475-2867 |
publishDate |
2020-07-01 |
description |
Abstract Background Osteosarcoma (OS) is the most common bone malignant tumor in children, youth, and adolescents. Circular RNA hsa_circ_0005909 (circ_0005909) is involved in the progression of OS. Nevertheless, there are few reports on the role and mechanism of circ_0005909 in OS. Methods Quantitative real-time polymerase chain reaction (qRT-PCR) was executed to examine the expression of circ_0005909, miR-936, and High Mobility Group Box 1 (HMGB1) mRNA in OS tissues and cells. Cell viability, colony formation, migration, and invasion were evaluated by Cell Counting Kit-8 (CCK-8), cell colony formation, or transwell assays. Cell epithelial-mesenchymal transition (EMT) and HMGB1 protein levels were assessed through western blot analysis. The role of circ_0005909 on tumor growth in vivo was verified by xenograft assay. The relationship between circ_0005909 or HMGB1 and miR-936 was confirmed with the dual-luciferase reporter or RNA pull-down assays. Results Circ_0005909 level was upregulated in OS tissues and cells. OS patients with high circ_0005909 expression had a lower survival rate. Circ_0005909 inhibition reduced tumor growth in vivo and constrained cell viability, colony formation, migration, invasion, and EMT of OS cells in vitro. Furthermore, circ_0005909 served as a sponge for miR-936 and the repressive impacts of circ_0005909 silencing on malignant behaviors of OS cells were abolished by miR-936 inhibitors. Also, HMGB1 acted as a target for miR-936 and was modulated by circ_0005909 via miR-936. Additionally, HMGB1 overexpression restored the inhibitory influence on the malignant behaviors of OS cells mediated by circ_0005909 inhibition. Conclusions Circ_0005909 inhibition impeded the progression of OS via downregulating HMGB1 via sponging miR-936. |
topic |
OS circ_0005909 miR-936 HMGB1 OS cell lines |
url |
http://link.springer.com/article/10.1186/s12935-020-01399-1 |
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