Pulp response of rats submitted to bleaching and the use of different anti-inflammatory drugs.
This study aimed to evaluate neuropeptide expression after bleaching treatment using histopathological and immunohistochemical analyses and the effects of hydrocortisone and acetaminophen on pulp inflammation, sine dental bleaching and inflammation first occur, and only then, the treatmentt. Sixty-t...
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doaj-3df3825a5926430fb6e01b2598d7d6f12021-03-03T20:58:59ZengPublic Library of Science (PLoS)PLoS ONE1932-62032019-01-01141e021033810.1371/journal.pone.0210338Pulp response of rats submitted to bleaching and the use of different anti-inflammatory drugs.Marjorie de Oliveira GallinariLuciano Tavares Ângelo CintraFrancine BenettiVanessa RahalEdilson ErvolinoAndré Luiz Fraga BrisoThis study aimed to evaluate neuropeptide expression after bleaching treatment using histopathological and immunohistochemical analyses and the effects of hydrocortisone and acetaminophen on pulp inflammation, sine dental bleaching and inflammation first occur, and only then, the treatmentt. Sixty-three rats were divided into three groups (n = 21) according to the pain-relieving therapy used: I-control; II-topical application of Otosporin for 10 min after the bleaching treatment; III-oral administration of paracetamol 30 min before whitening and then every 12h. In all the study groups, placebo gel was applied to the left upper jaw (control) and a 35% H2O2-based whitening gel was applied to the right upper jaw for 45 min. Seven animals from each group were euthanized at different time points: 0h after treatment, 24h, and 48h. After euthanasia, the first molar on each side was analyzed by histology and immunohistochemistry to assess the degree of inflammation and verify the presence of the neuropeptides, substance P (SP) and calcitonin gene-related peptide (CGRP). The data were analyzed using the statistical nonparametric Kruskal-Wallis test followed by Dunn's test for individual comparisons. Extensive areas of necrosis were observed in the groups that received bleaching treatment only, whereas reduced damage were obtained in the group treated with Otosporin. The immunohistochemical analysis showed positive immunolabeling in all groups, including the control, but this was stronger in the groups that received bleaching treatment. The best results were obtained in the group that received treatment with Otosporin. The use of Otosporin after dental bleaching minimized the side effects of this treatment.https://doi.org/10.1371/journal.pone.0210338 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Marjorie de Oliveira Gallinari Luciano Tavares Ângelo Cintra Francine Benetti Vanessa Rahal Edilson Ervolino André Luiz Fraga Briso |
spellingShingle |
Marjorie de Oliveira Gallinari Luciano Tavares Ângelo Cintra Francine Benetti Vanessa Rahal Edilson Ervolino André Luiz Fraga Briso Pulp response of rats submitted to bleaching and the use of different anti-inflammatory drugs. PLoS ONE |
author_facet |
Marjorie de Oliveira Gallinari Luciano Tavares Ângelo Cintra Francine Benetti Vanessa Rahal Edilson Ervolino André Luiz Fraga Briso |
author_sort |
Marjorie de Oliveira Gallinari |
title |
Pulp response of rats submitted to bleaching and the use of different anti-inflammatory drugs. |
title_short |
Pulp response of rats submitted to bleaching and the use of different anti-inflammatory drugs. |
title_full |
Pulp response of rats submitted to bleaching and the use of different anti-inflammatory drugs. |
title_fullStr |
Pulp response of rats submitted to bleaching and the use of different anti-inflammatory drugs. |
title_full_unstemmed |
Pulp response of rats submitted to bleaching and the use of different anti-inflammatory drugs. |
title_sort |
pulp response of rats submitted to bleaching and the use of different anti-inflammatory drugs. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2019-01-01 |
description |
This study aimed to evaluate neuropeptide expression after bleaching treatment using histopathological and immunohistochemical analyses and the effects of hydrocortisone and acetaminophen on pulp inflammation, sine dental bleaching and inflammation first occur, and only then, the treatmentt. Sixty-three rats were divided into three groups (n = 21) according to the pain-relieving therapy used: I-control; II-topical application of Otosporin for 10 min after the bleaching treatment; III-oral administration of paracetamol 30 min before whitening and then every 12h. In all the study groups, placebo gel was applied to the left upper jaw (control) and a 35% H2O2-based whitening gel was applied to the right upper jaw for 45 min. Seven animals from each group were euthanized at different time points: 0h after treatment, 24h, and 48h. After euthanasia, the first molar on each side was analyzed by histology and immunohistochemistry to assess the degree of inflammation and verify the presence of the neuropeptides, substance P (SP) and calcitonin gene-related peptide (CGRP). The data were analyzed using the statistical nonparametric Kruskal-Wallis test followed by Dunn's test for individual comparisons. Extensive areas of necrosis were observed in the groups that received bleaching treatment only, whereas reduced damage were obtained in the group treated with Otosporin. The immunohistochemical analysis showed positive immunolabeling in all groups, including the control, but this was stronger in the groups that received bleaching treatment. The best results were obtained in the group that received treatment with Otosporin. The use of Otosporin after dental bleaching minimized the side effects of this treatment. |
url |
https://doi.org/10.1371/journal.pone.0210338 |
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