The Effect of 5'-Adenylic Acid on Hepatic Proteome of Mice Radiated by 60Co γ-ray
Understanding the protection mechanism of 5'-AMP requires comprehensive knowledge of the proteins expressed during the period that the body is exposed to irradiation. Proteomics provides the tools for such analyses. Here, the experimental ICR mice were divided into three groups (normal group, m...
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doaj-3e02848529404cd7b8c247c8cce6fc782020-11-24T21:06:20ZengMDPI AGInternational Journal of Molecular Sciences1422-00672013-12-0115118620210.3390/ijms15010186ijms15010186The Effect of 5'-Adenylic Acid on Hepatic Proteome of Mice Radiated by 60Co γ-rayCuilin Cheng0Haitian Zhao1Zhenyu Wang2Weihong Lu3Lu Wang4Rongchun Wang5Lei Yao6School of Food Science and Engineering, Harbin Institute of Technology, Harbin 150090, ChinaSchool of Food Science and Engineering, Harbin Institute of Technology, Harbin 150090, ChinaSchool of Food Science and Engineering, Harbin Institute of Technology, Harbin 150090, ChinaSchool of Food Science and Engineering, Harbin Institute of Technology, Harbin 150090, ChinaSchool of Food Science and Engineering, Harbin Institute of Technology, Harbin 150090, ChinaSchool of Food Science and Engineering, Harbin Institute of Technology, Harbin 150090, ChinaSchool of Food Science and Engineering, Harbin Institute of Technology, Harbin 150090, ChinaUnderstanding the protection mechanism of 5'-AMP requires comprehensive knowledge of the proteins expressed during the period that the body is exposed to irradiation. Proteomics provides the tools for such analyses. Here, the experimental ICR mice were divided into three groups (normal group, model group and 5'-AMP + irradiation group). After different treatment, the hepatic total protein of each animal in three groups was separated by two-dimensional gel electrophoresis (2-DE). 2-DE analysis revealed fifty-eight protein spots were differentially expressed in comparison to three groups. From 58 protein spots, we selected nine spots to identify by MALDI-TOF-MS and received credible results. They were determined to be type I arginase, annexin A5, regucalcin, catalase, Tpm3 protein, Pdia4 protein, 14-3-3 protein epsilon, NAD-Malate dehydrogenase and heat shock protein 90. Considering the characteristic of these proteins, we proposed a possible protection pathway.http://www.mdpi.com/1422-0067/15/1/1865'-AMPprotective effectradiation damageproteomics |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Cuilin Cheng Haitian Zhao Zhenyu Wang Weihong Lu Lu Wang Rongchun Wang Lei Yao |
spellingShingle |
Cuilin Cheng Haitian Zhao Zhenyu Wang Weihong Lu Lu Wang Rongchun Wang Lei Yao The Effect of 5'-Adenylic Acid on Hepatic Proteome of Mice Radiated by 60Co γ-ray International Journal of Molecular Sciences 5'-AMP protective effect radiation damage proteomics |
author_facet |
Cuilin Cheng Haitian Zhao Zhenyu Wang Weihong Lu Lu Wang Rongchun Wang Lei Yao |
author_sort |
Cuilin Cheng |
title |
The Effect of 5'-Adenylic Acid on Hepatic Proteome of Mice Radiated by 60Co γ-ray |
title_short |
The Effect of 5'-Adenylic Acid on Hepatic Proteome of Mice Radiated by 60Co γ-ray |
title_full |
The Effect of 5'-Adenylic Acid on Hepatic Proteome of Mice Radiated by 60Co γ-ray |
title_fullStr |
The Effect of 5'-Adenylic Acid on Hepatic Proteome of Mice Radiated by 60Co γ-ray |
title_full_unstemmed |
The Effect of 5'-Adenylic Acid on Hepatic Proteome of Mice Radiated by 60Co γ-ray |
title_sort |
effect of 5'-adenylic acid on hepatic proteome of mice radiated by 60co γ-ray |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1422-0067 |
publishDate |
2013-12-01 |
description |
Understanding the protection mechanism of 5'-AMP requires comprehensive knowledge of the proteins expressed during the period that the body is exposed to irradiation. Proteomics provides the tools for such analyses. Here, the experimental ICR mice were divided into three groups (normal group, model group and 5'-AMP + irradiation group). After different treatment, the hepatic total protein of each animal in three groups was separated by two-dimensional gel electrophoresis (2-DE). 2-DE analysis revealed fifty-eight protein spots were differentially expressed in comparison to three groups. From 58 protein spots, we selected nine spots to identify by MALDI-TOF-MS and received credible results. They were determined to be type I arginase, annexin A5, regucalcin, catalase, Tpm3 protein, Pdia4 protein, 14-3-3 protein epsilon, NAD-Malate dehydrogenase and heat shock protein 90. Considering the characteristic of these proteins, we proposed a possible protection pathway. |
topic |
5'-AMP protective effect radiation damage proteomics |
url |
http://www.mdpi.com/1422-0067/15/1/186 |
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