Prospective validation obtained in a similar group of patients and with similar high throughput biological tests failed to confirm signatures for prediction of response to chemotherapy and survival in advanced NSCLC: a prospective study from the European Lung Cancer Working Party
Aim: Cisplatin doublets are standard 1st line treatment for advanced non small cell lung cancer (NSCLC), without accurate predictor for response and survival, but important toxicity. Our aims were to identify predictive (for response) and prognostic (for survival) biological signatures in patients w...
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doaj-3e0f351f2e7c49d59ba3ff908a0b1df02020-11-24T22:21:40ZengFrontiers Media S.A.Frontiers in Oncology2234-943X2015-01-01410.3389/fonc.2014.00386120953Prospective validation obtained in a similar group of patients and with similar high throughput biological tests failed to confirm signatures for prediction of response to chemotherapy and survival in advanced NSCLC: a prospective study from the European Lung Cancer Working PartyThierry eBerghmans0Lieveke eAmeye1Jean-Jacques eLafitte2Benoit eColinet3Alexis eCortot4Ingrid eCsToth5Stéphane eHolbrechts6Jacques eLecomte7Céline eMascaux8Anne-Pascale eMeert9Marianne ePaesmans10Michel eRichez11Arnaud eScherpereel12Christian eTulippe13Luc eWillems14Tiffany eDernies15Nathalie eLeclercq16JEAN PAUL eSCULIER17Institut Jules Bordet, Université Libre de Bruxelles (ULB)Université Libre de Bruxelles (ULB)CHU LilleGrand Hôpital de Charleroi, site Saint-JosephCHU LilleInstitut Jules Bordet, Université Libre de Bruxelles (ULB)Hôpital Ambroise ParéCH CharleroiPrincess Margaret Cancer CentreInstitut Jules Bordet, Université Libre de Bruxelles (ULB)Université Libre de Bruxelles (ULB)CHR St JosephCHU LilleCH MouscronUniversité de LiègeInstitut Jules Bordet, Université Libre de Bruxelles (ULB)Institut Jules Bordet, Université Libre de Bruxelles (ULB)Institut Jules Bordet, Université Libre de Bruxelles (ULB)Aim: Cisplatin doublets are standard 1st line treatment for advanced non small cell lung cancer (NSCLC), without accurate predictor for response and survival, but important toxicity. Our aims were to identify predictive (for response) and prognostic (for survival) biological signatures in patients with NSCLC using mRNA and miRNA expression.Methods: Patients with pathologically proven untreated NSCLC, receiving 1st line cisplatin-vinorelbine and with an assessable lesion were eligible. A bronchial biopsy was lysed into Tripure Isolation Reagent on ice, snap frozen and stored at -80°C. mRNA expression was analysed using microarrays Agilent Technologies. miRNA expression was assessed using TaqMan Low Density Arrays (756 human miR panel, Applied Biosystems). Validation was performed by RT-PCR on the selected genes. Survival was measured from the registration date and response assessed by WHO criteria.Results: Biopsies for transcriptomic analyses were obtained from 60 consecutive patients. No statistically significant differences were observed according to the main clinical characteristics, response rate (43% vs 41%) or survival (median 25 vs 29 months) between derivation and validation sets. In the derivation set (n=38 patients), two mRNA and one miRNA predictive signatures for response were obtained. One mRNA and one miRNA prognostic signatures were derived from the first set, allowing an adequate distinction of patients with good and poor overall and progression-free survivals. None of these signatures could be validated in the validation set (n=22 patients).Conclusions: In this prospective study with advanced NSCLC treated with cisplatin-vinorelbine, we were able to derive with high throughput techniques predictive and prognostic signatures based on transcriptomic analyses. However, these results could not be reproduced in an independent validation set. The role of miRNA and mRNA as predictive or prognostic factors remains a research topic and the use of high throhttp://journal.frontiersin.org/Journal/10.3389/fonc.2014.00386/fullSurvivalchemotherapymiRNAmRNANon-small cell lung cancer |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Thierry eBerghmans Lieveke eAmeye Jean-Jacques eLafitte Benoit eColinet Alexis eCortot Ingrid eCsToth Stéphane eHolbrechts Jacques eLecomte Céline eMascaux Anne-Pascale eMeert Marianne ePaesmans Michel eRichez Arnaud eScherpereel Christian eTulippe Luc eWillems Tiffany eDernies Nathalie eLeclercq JEAN PAUL eSCULIER |
spellingShingle |
Thierry eBerghmans Lieveke eAmeye Jean-Jacques eLafitte Benoit eColinet Alexis eCortot Ingrid eCsToth Stéphane eHolbrechts Jacques eLecomte Céline eMascaux Anne-Pascale eMeert Marianne ePaesmans Michel eRichez Arnaud eScherpereel Christian eTulippe Luc eWillems Tiffany eDernies Nathalie eLeclercq JEAN PAUL eSCULIER Prospective validation obtained in a similar group of patients and with similar high throughput biological tests failed to confirm signatures for prediction of response to chemotherapy and survival in advanced NSCLC: a prospective study from the European Lung Cancer Working Party Frontiers in Oncology Survival chemotherapy miRNA mRNA Non-small cell lung cancer |
author_facet |
Thierry eBerghmans Lieveke eAmeye Jean-Jacques eLafitte Benoit eColinet Alexis eCortot Ingrid eCsToth Stéphane eHolbrechts Jacques eLecomte Céline eMascaux Anne-Pascale eMeert Marianne ePaesmans Michel eRichez Arnaud eScherpereel Christian eTulippe Luc eWillems Tiffany eDernies Nathalie eLeclercq JEAN PAUL eSCULIER |
author_sort |
Thierry eBerghmans |
title |
Prospective validation obtained in a similar group of patients and with similar high throughput biological tests failed to confirm signatures for prediction of response to chemotherapy and survival in advanced NSCLC: a prospective study from the European Lung Cancer Working Party |
title_short |
Prospective validation obtained in a similar group of patients and with similar high throughput biological tests failed to confirm signatures for prediction of response to chemotherapy and survival in advanced NSCLC: a prospective study from the European Lung Cancer Working Party |
title_full |
Prospective validation obtained in a similar group of patients and with similar high throughput biological tests failed to confirm signatures for prediction of response to chemotherapy and survival in advanced NSCLC: a prospective study from the European Lung Cancer Working Party |
title_fullStr |
Prospective validation obtained in a similar group of patients and with similar high throughput biological tests failed to confirm signatures for prediction of response to chemotherapy and survival in advanced NSCLC: a prospective study from the European Lung Cancer Working Party |
title_full_unstemmed |
Prospective validation obtained in a similar group of patients and with similar high throughput biological tests failed to confirm signatures for prediction of response to chemotherapy and survival in advanced NSCLC: a prospective study from the European Lung Cancer Working Party |
title_sort |
prospective validation obtained in a similar group of patients and with similar high throughput biological tests failed to confirm signatures for prediction of response to chemotherapy and survival in advanced nsclc: a prospective study from the european lung cancer working party |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Oncology |
issn |
2234-943X |
publishDate |
2015-01-01 |
description |
Aim: Cisplatin doublets are standard 1st line treatment for advanced non small cell lung cancer (NSCLC), without accurate predictor for response and survival, but important toxicity. Our aims were to identify predictive (for response) and prognostic (for survival) biological signatures in patients with NSCLC using mRNA and miRNA expression.Methods: Patients with pathologically proven untreated NSCLC, receiving 1st line cisplatin-vinorelbine and with an assessable lesion were eligible. A bronchial biopsy was lysed into Tripure Isolation Reagent on ice, snap frozen and stored at -80°C. mRNA expression was analysed using microarrays Agilent Technologies. miRNA expression was assessed using TaqMan Low Density Arrays (756 human miR panel, Applied Biosystems). Validation was performed by RT-PCR on the selected genes. Survival was measured from the registration date and response assessed by WHO criteria.Results: Biopsies for transcriptomic analyses were obtained from 60 consecutive patients. No statistically significant differences were observed according to the main clinical characteristics, response rate (43% vs 41%) or survival (median 25 vs 29 months) between derivation and validation sets. In the derivation set (n=38 patients), two mRNA and one miRNA predictive signatures for response were obtained. One mRNA and one miRNA prognostic signatures were derived from the first set, allowing an adequate distinction of patients with good and poor overall and progression-free survivals. None of these signatures could be validated in the validation set (n=22 patients).Conclusions: In this prospective study with advanced NSCLC treated with cisplatin-vinorelbine, we were able to derive with high throughput techniques predictive and prognostic signatures based on transcriptomic analyses. However, these results could not be reproduced in an independent validation set. The role of miRNA and mRNA as predictive or prognostic factors remains a research topic and the use of high thro |
topic |
Survival chemotherapy miRNA mRNA Non-small cell lung cancer |
url |
http://journal.frontiersin.org/Journal/10.3389/fonc.2014.00386/full |
work_keys_str_mv |
AT thierryeberghmans prospectivevalidationobtainedinasimilargroupofpatientsandwithsimilarhighthroughputbiologicaltestsfailedtoconfirmsignaturesforpredictionofresponsetochemotherapyandsurvivalinadvancednsclcaprospectivestudyfromtheeuropeanlungcancerworkingparty AT lievekeeameye prospectivevalidationobtainedinasimilargroupofpatientsandwithsimilarhighthroughputbiologicaltestsfailedtoconfirmsignaturesforpredictionofresponsetochemotherapyandsurvivalinadvancednsclcaprospectivestudyfromtheeuropeanlungcancerworkingparty AT jeanjacqueselafitte prospectivevalidationobtainedinasimilargroupofpatientsandwithsimilarhighthroughputbiologicaltestsfailedtoconfirmsignaturesforpredictionofresponsetochemotherapyandsurvivalinadvancednsclcaprospectivestudyfromtheeuropeanlungcancerworkingparty AT benoitecolinet prospectivevalidationobtainedinasimilargroupofpatientsandwithsimilarhighthroughputbiologicaltestsfailedtoconfirmsignaturesforpredictionofresponsetochemotherapyandsurvivalinadvancednsclcaprospectivestudyfromtheeuropeanlungcancerworkingparty AT alexisecortot prospectivevalidationobtainedinasimilargroupofpatientsandwithsimilarhighthroughputbiologicaltestsfailedtoconfirmsignaturesforpredictionofresponsetochemotherapyandsurvivalinadvancednsclcaprospectivestudyfromtheeuropeanlungcancerworkingparty AT ingridecstoth prospectivevalidationobtainedinasimilargroupofpatientsandwithsimilarhighthroughputbiologicaltestsfailedtoconfirmsignaturesforpredictionofresponsetochemotherapyandsurvivalinadvancednsclcaprospectivestudyfromtheeuropeanlungcancerworkingparty AT stephaneeholbrechts prospectivevalidationobtainedinasimilargroupofpatientsandwithsimilarhighthroughputbiologicaltestsfailedtoconfirmsignaturesforpredictionofresponsetochemotherapyandsurvivalinadvancednsclcaprospectivestudyfromtheeuropeanlungcancerworkingparty AT jacqueselecomte prospectivevalidationobtainedinasimilargroupofpatientsandwithsimilarhighthroughputbiologicaltestsfailedtoconfirmsignaturesforpredictionofresponsetochemotherapyandsurvivalinadvancednsclcaprospectivestudyfromtheeuropeanlungcancerworkingparty AT celineemascaux prospectivevalidationobtainedinasimilargroupofpatientsandwithsimilarhighthroughputbiologicaltestsfailedtoconfirmsignaturesforpredictionofresponsetochemotherapyandsurvivalinadvancednsclcaprospectivestudyfromtheeuropeanlungcancerworkingparty AT annepascaleemeert prospectivevalidationobtainedinasimilargroupofpatientsandwithsimilarhighthroughputbiologicaltestsfailedtoconfirmsignaturesforpredictionofresponsetochemotherapyandsurvivalinadvancednsclcaprospectivestudyfromtheeuropeanlungcancerworkingparty AT marianneepaesmans prospectivevalidationobtainedinasimilargroupofpatientsandwithsimilarhighthroughputbiologicaltestsfailedtoconfirmsignaturesforpredictionofresponsetochemotherapyandsurvivalinadvancednsclcaprospectivestudyfromtheeuropeanlungcancerworkingparty AT michelerichez prospectivevalidationobtainedinasimilargroupofpatientsandwithsimilarhighthroughputbiologicaltestsfailedtoconfirmsignaturesforpredictionofresponsetochemotherapyandsurvivalinadvancednsclcaprospectivestudyfromtheeuropeanlungcancerworkingparty AT arnaudescherpereel prospectivevalidationobtainedinasimilargroupofpatientsandwithsimilarhighthroughputbiologicaltestsfailedtoconfirmsignaturesforpredictionofresponsetochemotherapyandsurvivalinadvancednsclcaprospectivestudyfromtheeuropeanlungcancerworkingparty AT christianetulippe prospectivevalidationobtainedinasimilargroupofpatientsandwithsimilarhighthroughputbiologicaltestsfailedtoconfirmsignaturesforpredictionofresponsetochemotherapyandsurvivalinadvancednsclcaprospectivestudyfromtheeuropeanlungcancerworkingparty AT lucewillems prospectivevalidationobtainedinasimilargroupofpatientsandwithsimilarhighthroughputbiologicaltestsfailedtoconfirmsignaturesforpredictionofresponsetochemotherapyandsurvivalinadvancednsclcaprospectivestudyfromtheeuropeanlungcancerworkingparty AT tiffanyedernies prospectivevalidationobtainedinasimilargroupofpatientsandwithsimilarhighthroughputbiologicaltestsfailedtoconfirmsignaturesforpredictionofresponsetochemotherapyandsurvivalinadvancednsclcaprospectivestudyfromtheeuropeanlungcancerworkingparty AT nathalieeleclercq prospectivevalidationobtainedinasimilargroupofpatientsandwithsimilarhighthroughputbiologicaltestsfailedtoconfirmsignaturesforpredictionofresponsetochemotherapyandsurvivalinadvancednsclcaprospectivestudyfromtheeuropeanlungcancerworkingparty AT jeanpaulesculier prospectivevalidationobtainedinasimilargroupofpatientsandwithsimilarhighthroughputbiologicaltestsfailedtoconfirmsignaturesforpredictionofresponsetochemotherapyandsurvivalinadvancednsclcaprospectivestudyfromtheeuropeanlungcancerworkingparty |
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