Integrated Analysis of Transcriptome and Metabolome and Evaluation of Antioxidant Activities in <i>Lavandula</i><i>pubescens</i>
<i>Lavandula pubescens,</i> belonging to the Labiatae family, is a newly discovered strongly aromatic species of lavender that is potentially beneficial for human health. Given the economic importance of lavender species, we sought in this study to characterize the terpenoid biosynthesis...
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doaj-3e1222ad1321494d921e614da1f93f682021-07-23T13:28:32ZengMDPI AGAntioxidants2076-39212021-06-01101027102710.3390/antiox10071027Integrated Analysis of Transcriptome and Metabolome and Evaluation of Antioxidant Activities in <i>Lavandula</i><i>pubescens</i>Chang Ha Park0Hyeon Ji Yeo1Ye Eun Park2Ye Jin Kim3Chanung Park4Jae Kwang Kim5Sang Un Park6Department of Crop Science, Chungnam National University, 99 Daehak-ro, Yuseong-gu, Daejeon 34134, KoreaDepartment of Crop Science, Chungnam National University, 99 Daehak-ro, Yuseong-gu, Daejeon 34134, KoreaDepartment of Crop Science, Chungnam National University, 99 Daehak-ro, Yuseong-gu, Daejeon 34134, KoreaDivision of Life Sciences, College of Life Sciences and Bioengineering, Incheon National University, Yeonsugu, Incheon 22012, KoreaDepartment of Botany and Plant Pathology, Purdue University, 915 West State Street, West Lafayette, IN 47907, USADivision of Life Sciences, College of Life Sciences and Bioengineering, Incheon National University, Yeonsugu, Incheon 22012, KoreaDepartment of Crop Science, Chungnam National University, 99 Daehak-ro, Yuseong-gu, Daejeon 34134, Korea<i>Lavandula pubescens,</i> belonging to the Labiatae family, is a newly discovered strongly aromatic species of lavender that is potentially beneficial for human health. Given the economic importance of lavender species, we sought in this study to characterize the terpenoid biosynthesis of <i>L. pubescens</i> by obtaining transcriptomic and metabolic datasets. Transcriptome analysis of <i>L. pubescens</i> grown aseptically in tissue culture medium yielded 124,233 unigenes with an average length of 470 bp and N50 value of 522 bp from 9,476,122,928 raw reads. In order to provide relevant biological information, the unigenes were annotated using the following public databases: National Center for Biotechnology Information (NCBI) nucleotide (NT) and non-redundant protein (NR), Brassica (BRAD), Arabidopsis Information Resource (TAIR), Clusters of Orthologous Groups (COG), and Gene Ontology (GO). NR annotation results revealed that <i>L. pubescens</i> is genetically closely related to <i>Sesamum indicum.</i> On the basis of the transcriptome data, a total of 14 cDNA clones encoding the terpene biosynthetic genes <i>LpDXS</i>, <i>LpMCT</i>, <i>LpMCS</i>, <i>LpHDR</i>, <i>LpIDI</i>, <i>LpAACT</i>, <i>LpHMGS</i>, <i>LpHMGR</i>, <i>LpMVK</i>, <i>LpPMK</i>, <i>LpMVD</i>, <i>LpGPPS</i>, <i>LpSQS</i>, and <i>LpGGPPS</i> were identified in <i>L. pubescens</i>. These were quantified in the roots, stems, and leaves of <i>L. pubescens</i> using quantitative real-time polymerase chain reaction (qRT-PCR), which revealed that the gene expression levels were higher in the leaves and stems than in the roots, which was found to be consistent with the levels of ursolic and oleanolic acids in the different organs using high-performance liquid chromatography (HPLC). A total of 48 hydrophilic metabolites were identified and quantified in the organs using gas chromatography time-of-flight mass spectrometry (GC-TOFMS). Furthermore, the antioxidant activity of an ethyl acetate extract of <i>L. pubescens</i> leaves was examined using different methods to determine the potential therapeutic properties. A reducing power assay revealed that the absorbance values increased in a concentration-dependent manner, whereas a 2,2-diphenyl-1-picrylhydrazyl radical scavenging assay indicated the strong activity (60.4 ± 0.9%) of the ethyl acetate extract at a concentration of 100 µg/mL, which also showed strong hydrogen peroxide (57.4 ± 2.7%), superoxide radical (62.1 ± 0.7%), and hydroxyl radical (58.6 ± 0.4%) scavenging activities.https://www.mdpi.com/2076-3921/10/7/1027<i>Lavandula pubescens</i>transcriptomemetabolomeantioxidant capacity |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Chang Ha Park Hyeon Ji Yeo Ye Eun Park Ye Jin Kim Chanung Park Jae Kwang Kim Sang Un Park |
spellingShingle |
Chang Ha Park Hyeon Ji Yeo Ye Eun Park Ye Jin Kim Chanung Park Jae Kwang Kim Sang Un Park Integrated Analysis of Transcriptome and Metabolome and Evaluation of Antioxidant Activities in <i>Lavandula</i><i>pubescens</i> Antioxidants <i>Lavandula pubescens</i> transcriptome metabolome antioxidant capacity |
author_facet |
Chang Ha Park Hyeon Ji Yeo Ye Eun Park Ye Jin Kim Chanung Park Jae Kwang Kim Sang Un Park |
author_sort |
Chang Ha Park |
title |
Integrated Analysis of Transcriptome and Metabolome and Evaluation of Antioxidant Activities in <i>Lavandula</i><i>pubescens</i> |
title_short |
Integrated Analysis of Transcriptome and Metabolome and Evaluation of Antioxidant Activities in <i>Lavandula</i><i>pubescens</i> |
title_full |
Integrated Analysis of Transcriptome and Metabolome and Evaluation of Antioxidant Activities in <i>Lavandula</i><i>pubescens</i> |
title_fullStr |
Integrated Analysis of Transcriptome and Metabolome and Evaluation of Antioxidant Activities in <i>Lavandula</i><i>pubescens</i> |
title_full_unstemmed |
Integrated Analysis of Transcriptome and Metabolome and Evaluation of Antioxidant Activities in <i>Lavandula</i><i>pubescens</i> |
title_sort |
integrated analysis of transcriptome and metabolome and evaluation of antioxidant activities in <i>lavandula</i><i>pubescens</i> |
publisher |
MDPI AG |
series |
Antioxidants |
issn |
2076-3921 |
publishDate |
2021-06-01 |
description |
<i>Lavandula pubescens,</i> belonging to the Labiatae family, is a newly discovered strongly aromatic species of lavender that is potentially beneficial for human health. Given the economic importance of lavender species, we sought in this study to characterize the terpenoid biosynthesis of <i>L. pubescens</i> by obtaining transcriptomic and metabolic datasets. Transcriptome analysis of <i>L. pubescens</i> grown aseptically in tissue culture medium yielded 124,233 unigenes with an average length of 470 bp and N50 value of 522 bp from 9,476,122,928 raw reads. In order to provide relevant biological information, the unigenes were annotated using the following public databases: National Center for Biotechnology Information (NCBI) nucleotide (NT) and non-redundant protein (NR), Brassica (BRAD), Arabidopsis Information Resource (TAIR), Clusters of Orthologous Groups (COG), and Gene Ontology (GO). NR annotation results revealed that <i>L. pubescens</i> is genetically closely related to <i>Sesamum indicum.</i> On the basis of the transcriptome data, a total of 14 cDNA clones encoding the terpene biosynthetic genes <i>LpDXS</i>, <i>LpMCT</i>, <i>LpMCS</i>, <i>LpHDR</i>, <i>LpIDI</i>, <i>LpAACT</i>, <i>LpHMGS</i>, <i>LpHMGR</i>, <i>LpMVK</i>, <i>LpPMK</i>, <i>LpMVD</i>, <i>LpGPPS</i>, <i>LpSQS</i>, and <i>LpGGPPS</i> were identified in <i>L. pubescens</i>. These were quantified in the roots, stems, and leaves of <i>L. pubescens</i> using quantitative real-time polymerase chain reaction (qRT-PCR), which revealed that the gene expression levels were higher in the leaves and stems than in the roots, which was found to be consistent with the levels of ursolic and oleanolic acids in the different organs using high-performance liquid chromatography (HPLC). A total of 48 hydrophilic metabolites were identified and quantified in the organs using gas chromatography time-of-flight mass spectrometry (GC-TOFMS). Furthermore, the antioxidant activity of an ethyl acetate extract of <i>L. pubescens</i> leaves was examined using different methods to determine the potential therapeutic properties. A reducing power assay revealed that the absorbance values increased in a concentration-dependent manner, whereas a 2,2-diphenyl-1-picrylhydrazyl radical scavenging assay indicated the strong activity (60.4 ± 0.9%) of the ethyl acetate extract at a concentration of 100 µg/mL, which also showed strong hydrogen peroxide (57.4 ± 2.7%), superoxide radical (62.1 ± 0.7%), and hydroxyl radical (58.6 ± 0.4%) scavenging activities. |
topic |
<i>Lavandula pubescens</i> transcriptome metabolome antioxidant capacity |
url |
https://www.mdpi.com/2076-3921/10/7/1027 |
work_keys_str_mv |
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