3-D worm tracker for freely moving C. elegans.
The manner in which the nervous system regulates animal behaviors in natural environments is a fundamental issue in biology. To address this question, C. elegans has been widely used as a model animal for the analysis of various animal behaviors. Previous behavioral assays have been limited to two-d...
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doaj-3e1580408125407980a35a389fa499302020-11-25T00:12:15ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0182e5748410.1371/journal.pone.00574843-D worm tracker for freely moving C. elegans.Namseop KwonJaeyeon PyoSeung-Jae LeeJung Ho JeThe manner in which the nervous system regulates animal behaviors in natural environments is a fundamental issue in biology. To address this question, C. elegans has been widely used as a model animal for the analysis of various animal behaviors. Previous behavioral assays have been limited to two-dimensional (2-D) environments, confining the worm motion to a planar substrate that does not reflect three-dimensional (3-D) natural environments such as rotting fruits or soil. Here, we develop a 3-D worm tracker (3DWT) for freely moving C. elegans in 3-D environments, based on a stereoscopic configuration. The 3DWT provides us with a quantitative trajectory, including the position and movement direction of the worm in 3-D. The 3DWT is also capable of recording and visualizing postures of the moving worm in 3-D, which are more complex than those in 2-D. Our 3DWT affords new opportunities for understanding the nervous system function that regulates animal behaviors in natural 3-D environments.http://europepmc.org/articles/PMC3578814?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Namseop Kwon Jaeyeon Pyo Seung-Jae Lee Jung Ho Je |
spellingShingle |
Namseop Kwon Jaeyeon Pyo Seung-Jae Lee Jung Ho Je 3-D worm tracker for freely moving C. elegans. PLoS ONE |
author_facet |
Namseop Kwon Jaeyeon Pyo Seung-Jae Lee Jung Ho Je |
author_sort |
Namseop Kwon |
title |
3-D worm tracker for freely moving C. elegans. |
title_short |
3-D worm tracker for freely moving C. elegans. |
title_full |
3-D worm tracker for freely moving C. elegans. |
title_fullStr |
3-D worm tracker for freely moving C. elegans. |
title_full_unstemmed |
3-D worm tracker for freely moving C. elegans. |
title_sort |
3-d worm tracker for freely moving c. elegans. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2013-01-01 |
description |
The manner in which the nervous system regulates animal behaviors in natural environments is a fundamental issue in biology. To address this question, C. elegans has been widely used as a model animal for the analysis of various animal behaviors. Previous behavioral assays have been limited to two-dimensional (2-D) environments, confining the worm motion to a planar substrate that does not reflect three-dimensional (3-D) natural environments such as rotting fruits or soil. Here, we develop a 3-D worm tracker (3DWT) for freely moving C. elegans in 3-D environments, based on a stereoscopic configuration. The 3DWT provides us with a quantitative trajectory, including the position and movement direction of the worm in 3-D. The 3DWT is also capable of recording and visualizing postures of the moving worm in 3-D, which are more complex than those in 2-D. Our 3DWT affords new opportunities for understanding the nervous system function that regulates animal behaviors in natural 3-D environments. |
url |
http://europepmc.org/articles/PMC3578814?pdf=render |
work_keys_str_mv |
AT namseopkwon 3dwormtrackerforfreelymovingcelegans AT jaeyeonpyo 3dwormtrackerforfreelymovingcelegans AT seungjaelee 3dwormtrackerforfreelymovingcelegans AT junghoje 3dwormtrackerforfreelymovingcelegans |
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