A Pilot Study of Chronological Microbiota Changes in a Rat Apical Periodontitis Model
Apical periodontitis caused by microbial infection in the dental pulp is characterized by inflammation, destruction of the pulpal and periradicular tissues, and alveolar bone resorption. We analyzed the chronological changes in microbiota using a pyrosequencing-based approach combined with radiologi...
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doaj-3e195814cc954ccd85b5c21be54b8d422020-11-25T03:21:25ZengMDPI AGMicroorganisms2076-26072020-08-0181174117410.3390/microorganisms8081174A Pilot Study of Chronological Microbiota Changes in a Rat Apical Periodontitis ModelOk-Jin Park0Moon-Hee Jeong1Eun-Hye Lee2Mi-Ran Cho3Jaehong Hwang4Seungryong Cho5Cheol-Heui Yun6Seung Hyun Han7Sun-Young Kim8Department of Oral Microbiology and Immunology, DRI, and BK21 Plus Program, School of Dentistry, Seoul National University, Seoul 08826, KoreaDepartment of Conservative Dentistry and Dental Research Institute, School of Dentistry, Seoul National University, Seoul 03080, KoreaDepartment of Conservative Dentistry and Dental Research Institute, School of Dentistry, Seoul National University, Seoul 03080, KoreaDepartment of Conservative Dentistry and Dental Research Institute, School of Dentistry, Seoul National University, Seoul 03080, KoreaDepartment of Nuclear and Quantum Engineering, KAIST, Daejeon 34141, KoreaDepartment of Nuclear and Quantum Engineering, KAIST, Daejeon 34141, KoreaDepartment of Agricultural Biotechnology and Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul 08826, KoreaDepartment of Oral Microbiology and Immunology, DRI, and BK21 Plus Program, School of Dentistry, Seoul National University, Seoul 08826, KoreaDepartment of Conservative Dentistry and Dental Research Institute, School of Dentistry, Seoul National University, Seoul 03080, KoreaApical periodontitis caused by microbial infection in the dental pulp is characterized by inflammation, destruction of the pulpal and periradicular tissues, and alveolar bone resorption. We analyzed the chronological changes in microbiota using a pyrosequencing-based approach combined with radiologic and histopathologic changes in a rat apical periodontitis model. During the three-week observation, the pulp and periapical area showed a typical progress of apical periodontitis. A total of 27 phyla, 645 genera, and 1276 species were identified. The root apex had a lower bacterial species diversity than the pulp chamber. Proteobacteria, Firmicutes, Bacteroidetes, and Actinobacteria were dominant phyla in both the pulp chamber and root apex. Remarkably, bacterial communities showed a tendency to change in the root apex based on the disease progression. At the genus level, <i>Escherichia, Streptococcus, Lactobacillus, Rodentibacter</i>, and <i>Bacteroidetes</i> were dominant genera in the pulp chamber. The most abundant genera in the root apex were <i>Bradyrhizobium, Halomonas</i>, and <i>Escherichia</i>. The species <i>Azospirillum oryzae</i> increased in the pulp chamber, whereas the species <i>Bradyrhizobium japonicum</i> and <i>Halomonas stevensii</i> were highly observed in the root apex as the disease progressed. The experimental rat model of apical periodontitis demonstrated a relationship between the microbiota and the apical periodontitis progression.https://www.mdpi.com/2076-2607/8/8/1174apical periodontitisRat apical periodontitis modelMicrobiotaIn vivo micro-computed tomography |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ok-Jin Park Moon-Hee Jeong Eun-Hye Lee Mi-Ran Cho Jaehong Hwang Seungryong Cho Cheol-Heui Yun Seung Hyun Han Sun-Young Kim |
spellingShingle |
Ok-Jin Park Moon-Hee Jeong Eun-Hye Lee Mi-Ran Cho Jaehong Hwang Seungryong Cho Cheol-Heui Yun Seung Hyun Han Sun-Young Kim A Pilot Study of Chronological Microbiota Changes in a Rat Apical Periodontitis Model Microorganisms apical periodontitis Rat apical periodontitis model Microbiota In vivo micro-computed tomography |
author_facet |
Ok-Jin Park Moon-Hee Jeong Eun-Hye Lee Mi-Ran Cho Jaehong Hwang Seungryong Cho Cheol-Heui Yun Seung Hyun Han Sun-Young Kim |
author_sort |
Ok-Jin Park |
title |
A Pilot Study of Chronological Microbiota Changes in a Rat Apical Periodontitis Model |
title_short |
A Pilot Study of Chronological Microbiota Changes in a Rat Apical Periodontitis Model |
title_full |
A Pilot Study of Chronological Microbiota Changes in a Rat Apical Periodontitis Model |
title_fullStr |
A Pilot Study of Chronological Microbiota Changes in a Rat Apical Periodontitis Model |
title_full_unstemmed |
A Pilot Study of Chronological Microbiota Changes in a Rat Apical Periodontitis Model |
title_sort |
pilot study of chronological microbiota changes in a rat apical periodontitis model |
publisher |
MDPI AG |
series |
Microorganisms |
issn |
2076-2607 |
publishDate |
2020-08-01 |
description |
Apical periodontitis caused by microbial infection in the dental pulp is characterized by inflammation, destruction of the pulpal and periradicular tissues, and alveolar bone resorption. We analyzed the chronological changes in microbiota using a pyrosequencing-based approach combined with radiologic and histopathologic changes in a rat apical periodontitis model. During the three-week observation, the pulp and periapical area showed a typical progress of apical periodontitis. A total of 27 phyla, 645 genera, and 1276 species were identified. The root apex had a lower bacterial species diversity than the pulp chamber. Proteobacteria, Firmicutes, Bacteroidetes, and Actinobacteria were dominant phyla in both the pulp chamber and root apex. Remarkably, bacterial communities showed a tendency to change in the root apex based on the disease progression. At the genus level, <i>Escherichia, Streptococcus, Lactobacillus, Rodentibacter</i>, and <i>Bacteroidetes</i> were dominant genera in the pulp chamber. The most abundant genera in the root apex were <i>Bradyrhizobium, Halomonas</i>, and <i>Escherichia</i>. The species <i>Azospirillum oryzae</i> increased in the pulp chamber, whereas the species <i>Bradyrhizobium japonicum</i> and <i>Halomonas stevensii</i> were highly observed in the root apex as the disease progressed. The experimental rat model of apical periodontitis demonstrated a relationship between the microbiota and the apical periodontitis progression. |
topic |
apical periodontitis Rat apical periodontitis model Microbiota In vivo micro-computed tomography |
url |
https://www.mdpi.com/2076-2607/8/8/1174 |
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