Occurrence and antimicrobial resistance patterns of Listeria monocytogenes isolated from vegetables
Abstract Although the consumption of fresh and minimally processed vegetables is considered healthy, outbreaks related to the contamination of these products are frequently reported. Among the food-borne pathogens that contaminate vegetables is Listeria monocytogenes, a ubiquitous organism that exhi...
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2016-06-01
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doaj-3e2e01aee69a4918b26f26c918930ccb2020-11-24T21:18:19ZengSociedade Brasileira de MicrobiologiaBrazilian Journal of Microbiology1678-44052016-06-0147243844310.1016/j.bjm.2015.11.033S1517-83822016000200438Occurrence and antimicrobial resistance patterns of Listeria monocytogenes isolated from vegetablesVanessa de Vasconcelos ByrneErnesto HoferDeyse Christina VallimRogeria Comastri de Castro AlmeidaAbstract Although the consumption of fresh and minimally processed vegetables is considered healthy, outbreaks related to the contamination of these products are frequently reported. Among the food-borne pathogens that contaminate vegetables is Listeria monocytogenes, a ubiquitous organism that exhibits the ability to survive and multiply at refrigerated temperatures. This study aimed to evaluate the occurrence of L. monocytogenes in vegetables as well as the antimicrobial resistance of isolates. The results showed that 3.03% of samples were contaminated with L. monocytogenes, comprising 2.22% of raw vegetables and 5.56% of ready-to-eat vegetables. Multiplex PCR confirmed the virulence potential of the isolates. Antimicrobial resistance profiling showed that 50% of the isolates were susceptible to the antibiotics used. The resistance of one isolate to penicillin G, a commonly employed therapeutic agent, and the presence of serotype 4b, a serotype commonly associated with food-borne outbreaks, could be potential health hazards for consumers.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000200438&lng=en&tlng=enVegetablesFood safetyListeria monocytogenesAntimicrobial resistance |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Vanessa de Vasconcelos Byrne Ernesto Hofer Deyse Christina Vallim Rogeria Comastri de Castro Almeida |
spellingShingle |
Vanessa de Vasconcelos Byrne Ernesto Hofer Deyse Christina Vallim Rogeria Comastri de Castro Almeida Occurrence and antimicrobial resistance patterns of Listeria monocytogenes isolated from vegetables Brazilian Journal of Microbiology Vegetables Food safety Listeria monocytogenes Antimicrobial resistance |
author_facet |
Vanessa de Vasconcelos Byrne Ernesto Hofer Deyse Christina Vallim Rogeria Comastri de Castro Almeida |
author_sort |
Vanessa de Vasconcelos Byrne |
title |
Occurrence and antimicrobial resistance patterns of Listeria monocytogenes isolated from vegetables |
title_short |
Occurrence and antimicrobial resistance patterns of Listeria monocytogenes isolated from vegetables |
title_full |
Occurrence and antimicrobial resistance patterns of Listeria monocytogenes isolated from vegetables |
title_fullStr |
Occurrence and antimicrobial resistance patterns of Listeria monocytogenes isolated from vegetables |
title_full_unstemmed |
Occurrence and antimicrobial resistance patterns of Listeria monocytogenes isolated from vegetables |
title_sort |
occurrence and antimicrobial resistance patterns of listeria monocytogenes isolated from vegetables |
publisher |
Sociedade Brasileira de Microbiologia |
series |
Brazilian Journal of Microbiology |
issn |
1678-4405 |
publishDate |
2016-06-01 |
description |
Abstract Although the consumption of fresh and minimally processed vegetables is considered healthy, outbreaks related to the contamination of these products are frequently reported. Among the food-borne pathogens that contaminate vegetables is Listeria monocytogenes, a ubiquitous organism that exhibits the ability to survive and multiply at refrigerated temperatures. This study aimed to evaluate the occurrence of L. monocytogenes in vegetables as well as the antimicrobial resistance of isolates. The results showed that 3.03% of samples were contaminated with L. monocytogenes, comprising 2.22% of raw vegetables and 5.56% of ready-to-eat vegetables. Multiplex PCR confirmed the virulence potential of the isolates. Antimicrobial resistance profiling showed that 50% of the isolates were susceptible to the antibiotics used. The resistance of one isolate to penicillin G, a commonly employed therapeutic agent, and the presence of serotype 4b, a serotype commonly associated with food-borne outbreaks, could be potential health hazards for consumers. |
topic |
Vegetables Food safety Listeria monocytogenes Antimicrobial resistance |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000200438&lng=en&tlng=en |
work_keys_str_mv |
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1726009751653842944 |