Comparative Study on the Estimation of Estrous Cycle in Mice by Visual and Vaginal Lavage Method
Introduction: Evaluation of estrous cycle in laboratory animals can be a useful measure of the integrity of hypothalamic-pituitaryovarian reproductive axis. Assessment of vaginal cytology is crucial to assess the milieu and compare the endocrine status of animals among the experimental groups. A...
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doaj-3e3d8080b21c4b15862e4620a5fc08f52020-11-25T02:15:57ZengJCDR Research and Publications Private LimitedJournal of Clinical and Diagnostic Research2249-782X0973-709X2017-01-01111AC05AC0710.7860/JCDR/2017/23977.9148Comparative Study on the Estimation of Estrous Cycle in Mice by Visual and Vaginal Lavage MethodGnanagurudasan Ekambaram0Senthil Kumar Sampath Kumar1Leena Dennis Joseph2Ph.D Research Scholar, Department of Anatomy, Sri Ramachandra Medical College and Research Institute, Sri Ramachandra University, Chennai, Tamil Nadu, India.Professor, Department of Anatomy, Sri Ramachandra Medical College and Research Institute, Sri Ramachandra University, Chennai, Tamil Nadu, India.Professor, Department of Pathology, Sri Ramachandra Medical College and Research Institute, Sri Ramachandra University, Chennai, Tamil Nadu, India.Introduction: Evaluation of estrous cycle in laboratory animals can be a useful measure of the integrity of hypothalamic-pituitaryovarian reproductive axis. Assessment of vaginal cytology is crucial to assess the milieu and compare the endocrine status of animals among the experimental groups. Aim: The present study was attempted to compare the estimation of estrous cycle by visual method and non invasive vaginal lavage method. Materials and Methods: Sixty healthy female swiss albino mice were used for the present study. The appearance of the vagina with respect to the opening of vagina, vaginal swellings were observed. Non-invasive method was used in vaginal lavage method in which nucleated epithelial cells, cornified squamous epithelial cells and leucocytes present in vaginal smears were used to identify the estrous stages. Results: The estimation of estrous cycle by visual method coincides with the vaginal lavage method. In Vaginal lavage method, the accurate proportion of cells and the transition phases can be evaluated. Conclusion: The non-invasive method reduces the risk of pseudo -pregnancy and mechanical trauma. Though, visual method is quick and reliable, for accurate estimation of the stage of the estrous, non-invasive vaginal lavage method is ideal. https://jcdr.net/articles/PDF/9148/23977_CE(RA1)_F(T)_PF1(SRI_RK)_PF1(PI_RK)_PFA(AK)_PF2(RB_DK).pdflaboratory animalsreproductive cyclevaginal cell morphologyvaginal smears |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Gnanagurudasan Ekambaram Senthil Kumar Sampath Kumar Leena Dennis Joseph |
spellingShingle |
Gnanagurudasan Ekambaram Senthil Kumar Sampath Kumar Leena Dennis Joseph Comparative Study on the Estimation of Estrous Cycle in Mice by Visual and Vaginal Lavage Method Journal of Clinical and Diagnostic Research laboratory animals reproductive cycle vaginal cell morphology vaginal smears |
author_facet |
Gnanagurudasan Ekambaram Senthil Kumar Sampath Kumar Leena Dennis Joseph |
author_sort |
Gnanagurudasan Ekambaram |
title |
Comparative Study on the Estimation of Estrous Cycle in Mice by Visual and Vaginal Lavage Method |
title_short |
Comparative Study on the Estimation of Estrous Cycle in Mice by Visual and Vaginal Lavage Method |
title_full |
Comparative Study on the Estimation of Estrous Cycle in Mice by Visual and Vaginal Lavage Method |
title_fullStr |
Comparative Study on the Estimation of Estrous Cycle in Mice by Visual and Vaginal Lavage Method |
title_full_unstemmed |
Comparative Study on the Estimation of Estrous Cycle in Mice by Visual and Vaginal Lavage Method |
title_sort |
comparative study on the estimation of estrous cycle in mice by visual and vaginal lavage method |
publisher |
JCDR Research and Publications Private Limited |
series |
Journal of Clinical and Diagnostic Research |
issn |
2249-782X 0973-709X |
publishDate |
2017-01-01 |
description |
Introduction: Evaluation of estrous cycle in laboratory animals
can be a useful measure of the integrity of hypothalamic-pituitaryovarian reproductive axis. Assessment of vaginal cytology is
crucial to assess the milieu and compare the endocrine status
of animals among the experimental groups.
Aim: The present study was attempted to compare the
estimation of estrous cycle by visual method and non invasive
vaginal lavage method.
Materials and Methods: Sixty healthy female swiss albino mice
were used for the present study. The appearance of the vagina
with respect to the opening of vagina, vaginal swellings were
observed. Non-invasive method was used in vaginal lavage
method in which nucleated epithelial cells, cornified squamous
epithelial cells and leucocytes present in vaginal smears were
used to identify the estrous stages.
Results: The estimation of estrous cycle by visual method
coincides with the vaginal lavage method. In Vaginal lavage
method, the accurate proportion of cells and the transition
phases can be evaluated.
Conclusion: The non-invasive method reduces the risk of pseudo
-pregnancy and mechanical trauma. Though, visual method is
quick and reliable, for accurate estimation of the stage of the
estrous, non-invasive vaginal lavage method is ideal. |
topic |
laboratory animals reproductive cycle vaginal cell morphology vaginal smears |
url |
https://jcdr.net/articles/PDF/9148/23977_CE(RA1)_F(T)_PF1(SRI_RK)_PF1(PI_RK)_PFA(AK)_PF2(RB_DK).pdf |
work_keys_str_mv |
AT gnanagurudasanekambaram comparativestudyontheestimationofestrouscycleinmicebyvisualandvaginallavagemethod AT senthilkumarsampathkumar comparativestudyontheestimationofestrouscycleinmicebyvisualandvaginallavagemethod AT leenadennisjoseph comparativestudyontheestimationofestrouscycleinmicebyvisualandvaginallavagemethod |
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1724893846792306688 |