Anticancer activity and potential mechanisms of 1C, a ginseng saponin derivative, on prostate cancer cells

Background: AD-2 (20(R)-dammarane-3b, 12b, 20, 25-tetrol; 25-OH-PPD) is a ginsenoside and isolated from Panax ginseng, showing anticancer activity against extensive human cancer cell lines. In this study, effects and mechanisms of 1C ((20R)-3b-O-(L-alanyl)-dammarane-12b, 20, 25-triol), a modified ve...

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Main Authors:	Xu De Wang, Guang Yue Su, Chen Zhao, Fan Zhi Qu, Peng Wang, Yu Qing Zhao
Format:	Article
Language:	English
Published:	Elsevier 2018-04-01
Series:	Journal of Ginseng Research
Online Access:	http://www.sciencedirect.com/science/article/pii/S1226845316301154

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spelling	doaj-3e3fa436414e44abab8b178e51e9d9e52020-11-24T22:50:13ZengElsevierJournal of Ginseng Research1226-84532018-04-01422133143Anticancer activity and potential mechanisms of 1C, a ginseng saponin derivative, on prostate cancer cellsXu De Wang0Guang Yue Su1Chen Zhao2Fan Zhi Qu3Peng Wang4Yu Qing Zhao5School of Functional Food and Wine, Shenyang Pharmaceutical University, Shenyang, China; Key Laboratory of Structure-based Drug Design and Discovery of Education, Shenyang Pharmaceurical University, Shenyang, ChinaSchool of Functional Food and Wine, Shenyang Pharmaceutical University, Shenyang, China; Key Laboratory of Structure-based Drug Design and Discovery of Education, Shenyang Pharmaceurical University, Shenyang, ChinaKey Laboratory of Structure-based Drug Design and Discovery of Education, Shenyang Pharmaceurical University, Shenyang, China; College of Life Science and Biological Pharmaceutical, Shenyang Pharmaceutical University, Shenyang, ChinaSchool of Functional Food and Wine, Shenyang Pharmaceutical University, Shenyang, China; Key Laboratory of Structure-based Drug Design and Discovery of Education, Shenyang Pharmaceurical University, Shenyang, ChinaSchool of Functional Food and Wine, Shenyang Pharmaceutical University, Shenyang, China; Key Laboratory of Structure-based Drug Design and Discovery of Education, Shenyang Pharmaceurical University, Shenyang, ChinaSchool of Functional Food and Wine, Shenyang Pharmaceutical University, Shenyang, China; Key Laboratory of Structure-based Drug Design and Discovery of Education, Shenyang Pharmaceurical University, Shenyang, China; Corresponding author. School of Functional Food and Wine, Shenyang Pharmaceutical University, No. 103, Wenhua Road, Shenhe District, Shenyang 110016, Liaoning, China; Key Laboratory of Structure-based Drug Design and Discovery of Education, Shenyang Pharmaceurical University, No. 103, Wenhua Road, Shenhe District, Shenyang 110016, Liaoning, China.Background: AD-2 (20(R)-dammarane-3b, 12b, 20, 25-tetrol; 25-OH-PPD) is a ginsenoside and isolated from Panax ginseng, showing anticancer activity against extensive human cancer cell lines. In this study, effects and mechanisms of 1C ((20R)-3b-O-(L-alanyl)-dammarane-12b, 20, 25-triol), a modified version of AD-2, were evaluated for its development as a novel anticancer drug. Methods: MTT assay was performed to evaluate cell cytotoxic activity. Cell cycle and levels of reactive oxygen species (ROS) were determined using flow cytometry analysis. Western blotting was employed to analyze signaling pathways. Results: 1C concentration-dependently reduces prostate cancer cell viability without affecting normal human gastric epithelial cell line-1 viability. In LNCaP prostate cancer cells, 1C triggered apoptosis via Bcl-2 family-mediated mitochondria pathway, downregulated expression of mouse double minute 2, upregulated expression of p53 and stimulated ROS production. ROS scavenger, N-acetylcysteine, can attenuate 1C-induced apoptosis. 1C also inhibited the proliferation of LNCaP cells through inhibition on Wnt/β-catenin signaling pathway. Conclusion: 1C shows obvious anticancer activity based on inducing cell apoptosis by Bcl-2 family-mediated mitochondria pathway and ROS production, inhibiting Wnt/β-catenin signaling pathway. These findings demonstrate that 1C may provide leads as a potential agent for cancer therapy. Keywords: 1C, AD-2, apoptosis, reactive oxygen species, Wnt/β-catenin pathwayhttp://www.sciencedirect.com/science/article/pii/S1226845316301154
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Xu De Wang Guang Yue Su Chen Zhao Fan Zhi Qu Peng Wang Yu Qing Zhao
spellingShingle	Xu De Wang Guang Yue Su Chen Zhao Fan Zhi Qu Peng Wang Yu Qing Zhao Anticancer activity and potential mechanisms of 1C, a ginseng saponin derivative, on prostate cancer cells Journal of Ginseng Research
author_facet	Xu De Wang Guang Yue Su Chen Zhao Fan Zhi Qu Peng Wang Yu Qing Zhao
author_sort	Xu De Wang
title	Anticancer activity and potential mechanisms of 1C, a ginseng saponin derivative, on prostate cancer cells
title_short	Anticancer activity and potential mechanisms of 1C, a ginseng saponin derivative, on prostate cancer cells
title_full	Anticancer activity and potential mechanisms of 1C, a ginseng saponin derivative, on prostate cancer cells
title_fullStr	Anticancer activity and potential mechanisms of 1C, a ginseng saponin derivative, on prostate cancer cells
title_full_unstemmed	Anticancer activity and potential mechanisms of 1C, a ginseng saponin derivative, on prostate cancer cells
title_sort	anticancer activity and potential mechanisms of 1c, a ginseng saponin derivative, on prostate cancer cells
publisher	Elsevier
series	Journal of Ginseng Research
issn	1226-8453
publishDate	2018-04-01
description	Background: AD-2 (20(R)-dammarane-3b, 12b, 20, 25-tetrol; 25-OH-PPD) is a ginsenoside and isolated from Panax ginseng, showing anticancer activity against extensive human cancer cell lines. In this study, effects and mechanisms of 1C ((20R)-3b-O-(L-alanyl)-dammarane-12b, 20, 25-triol), a modified version of AD-2, were evaluated for its development as a novel anticancer drug. Methods: MTT assay was performed to evaluate cell cytotoxic activity. Cell cycle and levels of reactive oxygen species (ROS) were determined using flow cytometry analysis. Western blotting was employed to analyze signaling pathways. Results: 1C concentration-dependently reduces prostate cancer cell viability without affecting normal human gastric epithelial cell line-1 viability. In LNCaP prostate cancer cells, 1C triggered apoptosis via Bcl-2 family-mediated mitochondria pathway, downregulated expression of mouse double minute 2, upregulated expression of p53 and stimulated ROS production. ROS scavenger, N-acetylcysteine, can attenuate 1C-induced apoptosis. 1C also inhibited the proliferation of LNCaP cells through inhibition on Wnt/β-catenin signaling pathway. Conclusion: 1C shows obvious anticancer activity based on inducing cell apoptosis by Bcl-2 family-mediated mitochondria pathway and ROS production, inhibiting Wnt/β-catenin signaling pathway. These findings demonstrate that 1C may provide leads as a potential agent for cancer therapy. Keywords: 1C, AD-2, apoptosis, reactive oxygen species, Wnt/β-catenin pathway
url	http://www.sciencedirect.com/science/article/pii/S1226845316301154
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Anticancer activity and potential mechanisms of 1C, a ginseng saponin derivative, on prostate cancer cells

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