Study on ITS1 Gene of Iranian Trichomonas Vaginalis by Molecular Methods
Background: Trichomoniasis is a worldwide protozoan parasitic disease and metronidazole is a choice drug for its treatment. Because of disease importance in public health and its controversial ideas about the prevalence of drug resistance, this study was carried out.Methods: Fifty-two suspected vagi...
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Tehran University of Medical Sciences
2010-12-01
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doaj-3e5105982cce4309b588495f0df352c32021-03-02T05:53:24ZengTehran University of Medical SciencesIranian Journal of Parasitology1735-70202008-238X2010-12-0154914Study on ITS1 Gene of Iranian Trichomonas Vaginalis by Molecular MethodsF KazemiH HooshyarB ZareikarM BandehpourM ArbabiS TalariR AlizadehB KazemiBackground: Trichomoniasis is a worldwide protozoan parasitic disease and metronidazole is a choice drug for its treatment. Because of disease importance in public health and its controversial ideas about the prevalence of drug resistance, this study was carried out.Methods: Fifty-two suspected vaginal samples were collected from 2006 to 2007 in Gynecology Maryam Hospital, Tehran, Iran. All isolates were examined by microscopic, culture and PCR techniques. The PCR products were analyzed by RFLP and CSGE methods and two suspected samples were sequenced.Results: Trichomonas vaginalis was identified from all 52 samples. Of 52 isolates, 45 samples were successfully cultured and amplified by PCR except one. Seven were positive only by PCR. Finally, ITS1 fragment was successfully amplified in 51 of 52. CSGE analysis and PCR products digestion by MspI followed by sequencing showed nucleotide mutation at position 209 (C209T) of the ITS1 fragment in two (3.9%) of them.Conclusion: The results showed mutation in ITS1 fragment of T. vaginalis in two (3.9%) of Iranian isolates which may be related to metronidazole resistance.http://journals.tums.ac.ir/upload_files/pdf/17278.pdfTrichomonas VaginalisMutationITS1 Fragment |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
F Kazemi H Hooshyar B Zareikar M Bandehpour M Arbabi S Talari R Alizadeh B Kazemi |
spellingShingle |
F Kazemi H Hooshyar B Zareikar M Bandehpour M Arbabi S Talari R Alizadeh B Kazemi Study on ITS1 Gene of Iranian Trichomonas Vaginalis by Molecular Methods Iranian Journal of Parasitology Trichomonas Vaginalis Mutation ITS1 Fragment |
author_facet |
F Kazemi H Hooshyar B Zareikar M Bandehpour M Arbabi S Talari R Alizadeh B Kazemi |
author_sort |
F Kazemi |
title |
Study on ITS1 Gene of Iranian Trichomonas Vaginalis by Molecular Methods |
title_short |
Study on ITS1 Gene of Iranian Trichomonas Vaginalis by Molecular Methods |
title_full |
Study on ITS1 Gene of Iranian Trichomonas Vaginalis by Molecular Methods |
title_fullStr |
Study on ITS1 Gene of Iranian Trichomonas Vaginalis by Molecular Methods |
title_full_unstemmed |
Study on ITS1 Gene of Iranian Trichomonas Vaginalis by Molecular Methods |
title_sort |
study on its1 gene of iranian trichomonas vaginalis by molecular methods |
publisher |
Tehran University of Medical Sciences |
series |
Iranian Journal of Parasitology |
issn |
1735-7020 2008-238X |
publishDate |
2010-12-01 |
description |
Background: Trichomoniasis is a worldwide protozoan parasitic disease and metronidazole is a choice drug for its treatment. Because of disease importance in public health and its controversial ideas about the prevalence of drug resistance, this study was carried out.Methods: Fifty-two suspected vaginal samples were collected from 2006 to 2007 in Gynecology Maryam Hospital, Tehran, Iran. All isolates were examined by microscopic, culture and PCR techniques. The PCR products were analyzed by RFLP and CSGE methods and two suspected samples were sequenced.Results: Trichomonas vaginalis was identified from all 52 samples. Of 52 isolates, 45 samples were successfully cultured and amplified by PCR except one. Seven were positive only by PCR. Finally, ITS1 fragment was successfully amplified in 51 of 52. CSGE analysis and PCR products digestion by MspI followed by sequencing showed nucleotide mutation at position 209 (C209T) of the ITS1 fragment in two (3.9%) of them.Conclusion: The results showed mutation in ITS1 fragment of T. vaginalis in two (3.9%) of Iranian isolates which may be related to metronidazole resistance. |
topic |
Trichomonas Vaginalis Mutation ITS1 Fragment |
url |
http://journals.tums.ac.ir/upload_files/pdf/17278.pdf |
work_keys_str_mv |
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