Imaging neuropeptide release at synapses with a genetically engineered reporter

Research on neuropeptide function has advanced rapidly, yet there is still no spatio-temporally resolved method to measure the release of neuropeptides in vivo. Here we introduce Neuropeptide Release Reporters (NPRRs): novel genetically-encoded sensors with high temporal resolution and genetic speci...

Full description

Bibliographic Details
Main Authors:	Keke Ding, Yifu Han, Taylor W Seid, Christopher Buser, Tomomi Karigo, Shishuo Zhang, Dion K Dickman, David J Anderson
Format:	Article
Language:	English
Published:	eLife Sciences Publications Ltd 2019-06-01
Series:	eLife
Subjects:	neuropeptides dense core vesicle neurotransmission
Online Access:	https://elifesciences.org/articles/46421

id	doaj-3e6755a0ea4f477385aeff3df2676abb
record_format	Article
spelling	doaj-3e6755a0ea4f477385aeff3df2676abb2021-05-05T17:43:31ZengeLife Sciences Publications LtdeLife2050-084X2019-06-01810.7554/eLife.46421Imaging neuropeptide release at synapses with a genetically engineered reporterKeke Ding0https://orcid.org/0000-0002-5261-4843Yifu Han1https://orcid.org/0000-0002-1201-654XTaylor W Seid2Christopher Buser3https://orcid.org/0000-0002-4379-3878Tomomi Karigo4Shishuo Zhang5Dion K Dickman6https://orcid.org/0000-0003-1884-284XDavid J Anderson7https://orcid.org/0000-0001-6175-3872Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, United StatesDepartment of Neurobiology, University of Southern California, Los Angeles, United States; Neuroscience Graduate Program, University of Southern California, Los Angeles, United StatesDivision of Biology and Biological Engineering, California Institute of Technology, Pasadena, United StatesOak Crest Institute of Science, Monrovia, United StatesDivision of Biology and Biological Engineering, California Institute of Technology, Pasadena, United StatesDivision of Biology and Biological Engineering, California Institute of Technology, Pasadena, United StatesDepartment of Neurobiology, University of Southern California, Los Angeles, United StatesDivision of Biology and Biological Engineering, California Institute of Technology, Pasadena, United States; Howard Hughes Medical Institute, California Institute of Technology, Pasadena, United States; Tianqiao and Chrissy Chen Institute for Neuroscience, California Institute of Technology, Pasadena, United StatesResearch on neuropeptide function has advanced rapidly, yet there is still no spatio-temporally resolved method to measure the release of neuropeptides in vivo. Here we introduce Neuropeptide Release Reporters (NPRRs): novel genetically-encoded sensors with high temporal resolution and genetic specificity. Using the Drosophila larval neuromuscular junction (NMJ) as a model, we provide evidence that NPRRs recapitulate the trafficking and packaging of native neuropeptides, and report stimulation-evoked neuropeptide release events as real-time changes in fluorescence intensity, with sub-second temporal resolution.https://elifesciences.org/articles/46421neuropeptidesdense core vesicleneurotransmission
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Keke Ding Yifu Han Taylor W Seid Christopher Buser Tomomi Karigo Shishuo Zhang Dion K Dickman David J Anderson
spellingShingle	Keke Ding Yifu Han Taylor W Seid Christopher Buser Tomomi Karigo Shishuo Zhang Dion K Dickman David J Anderson Imaging neuropeptide release at synapses with a genetically engineered reporter eLife neuropeptides dense core vesicle neurotransmission
author_facet	Keke Ding Yifu Han Taylor W Seid Christopher Buser Tomomi Karigo Shishuo Zhang Dion K Dickman David J Anderson
author_sort	Keke Ding
title	Imaging neuropeptide release at synapses with a genetically engineered reporter
title_short	Imaging neuropeptide release at synapses with a genetically engineered reporter
title_full	Imaging neuropeptide release at synapses with a genetically engineered reporter
title_fullStr	Imaging neuropeptide release at synapses with a genetically engineered reporter
title_full_unstemmed	Imaging neuropeptide release at synapses with a genetically engineered reporter
title_sort	imaging neuropeptide release at synapses with a genetically engineered reporter
publisher	eLife Sciences Publications Ltd
series	eLife
issn	2050-084X
publishDate	2019-06-01
description	Research on neuropeptide function has advanced rapidly, yet there is still no spatio-temporally resolved method to measure the release of neuropeptides in vivo. Here we introduce Neuropeptide Release Reporters (NPRRs): novel genetically-encoded sensors with high temporal resolution and genetic specificity. Using the Drosophila larval neuromuscular junction (NMJ) as a model, we provide evidence that NPRRs recapitulate the trafficking and packaging of native neuropeptides, and report stimulation-evoked neuropeptide release events as real-time changes in fluorescence intensity, with sub-second temporal resolution.
topic	neuropeptides dense core vesicle neurotransmission
url	https://elifesciences.org/articles/46421
work_keys_str_mv	AT kekeding imagingneuropeptidereleaseatsynapseswithageneticallyengineeredreporter AT yifuhan imagingneuropeptidereleaseatsynapseswithageneticallyengineeredreporter AT taylorwseid imagingneuropeptidereleaseatsynapseswithageneticallyengineeredreporter AT christopherbuser imagingneuropeptidereleaseatsynapseswithageneticallyengineeredreporter AT tomomikarigo imagingneuropeptidereleaseatsynapseswithageneticallyengineeredreporter AT shishuozhang imagingneuropeptidereleaseatsynapseswithageneticallyengineeredreporter AT dionkdickman imagingneuropeptidereleaseatsynapseswithageneticallyengineeredreporter AT davidjanderson imagingneuropeptidereleaseatsynapseswithageneticallyengineeredreporter
_version_	1721459068983312384

Imaging neuropeptide release at synapses with a genetically engineered reporter

Similar Items