Loop-Mediated Isothermal Amplification untuk Mendeteksi Gen blaTEM sebagai Penyandi Extended-Spectrum Beta-Lactamase pada Isolat Enterobacteriaceae
Extended-spectrum beta-lactamase (ESBL) is a beta-lactamase enzyme that is capable of hydrolyzing penicillin, cephalosporin, and monobactam, and can be inhibited by clavulanic acid. This enzyme is encoded by multiple genes, one of them is blaTEM. Polymerase chain reaction (PCR) is one of the DNA amp...
Main Authors: | , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Universitas Padjajaran
2015-12-01
|
Series: | Majalah Kedokteran Bandung |
Subjects: | |
Online Access: | http://journal.fk.unpad.ac.id/index.php/mkb/article/view/618 |
id |
doaj-3e76140d7653476f9467f96872096f99 |
---|---|
record_format |
Article |
spelling |
doaj-3e76140d7653476f9467f96872096f992020-11-24T22:47:10ZengUniversitas PadjajaranMajalah Kedokteran Bandung0126-074X2338-62232015-12-01474242249Loop-Mediated Isothermal Amplification untuk Mendeteksi Gen blaTEM sebagai Penyandi Extended-Spectrum Beta-Lactamase pada Isolat EnterobacteriaceaeBayu A. P. Wilopo0Sunarjati Sudigdoadi1Edhyana Sahiratmadja2 Intan M. W. Dewi3Departemen Mikrobiologi dan Parasitologi, Fakultas Kedokteran, Universitas PadjadjaranDepartemen Mikrobiologi dan Parasitologi, Fakultas Kedokteran, Universitas PadjadjaranDepartemen Biokimia dan Biologi Molekuler, Fakultas Kedokteran, Universitas Padjadjaran Departemen Mikrobiologi dan Parasitologi, Fakultas Kedokteran, Universitas PadjadjaranExtended-spectrum beta-lactamase (ESBL) is a beta-lactamase enzyme that is capable of hydrolyzing penicillin, cephalosporin, and monobactam, and can be inhibited by clavulanic acid. This enzyme is encoded by multiple genes, one of them is blaTEM. Polymerase chain reaction (PCR) is one of the DNA amplification methods that are frequently used; however, there are other methods that can be used including, among others, loop-mediated isothermal amplification (LAMP). LAMP requires simple equipment with quicker and easy-to-read results compared to PCR. This study was a diagnostic test to explore the sensitivity and specificity of LAMP method compared to PCR in detecting blaTEM gene. Furthermore, the concordance between LAMP and PCR methods was assessed. A total of 92 Enterobacteriaceae isolates were examined by PCR and LAMP methods and compared. The result showed that the LAMP method had a sensitivity of 91.4% and a specificity of 91.2% with a concordance value (kappa) of 85.4%. In conclusion, LAMP method has a good validity and a very good conformity compared to the PCR method. Therefore, LAMP method can be used as an alternative diagnostic test, especially in limited settings. http://journal.fk.unpad.ac.id/index.php/mkb/article/view/618blaTEMEnterobacteriaceaeESBLLAMPPCR |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Bayu A. P. Wilopo Sunarjati Sudigdoadi Edhyana Sahiratmadja Intan M. W. Dewi |
spellingShingle |
Bayu A. P. Wilopo Sunarjati Sudigdoadi Edhyana Sahiratmadja Intan M. W. Dewi Loop-Mediated Isothermal Amplification untuk Mendeteksi Gen blaTEM sebagai Penyandi Extended-Spectrum Beta-Lactamase pada Isolat Enterobacteriaceae Majalah Kedokteran Bandung blaTEM Enterobacteriaceae ESBL LAMP PCR |
author_facet |
Bayu A. P. Wilopo Sunarjati Sudigdoadi Edhyana Sahiratmadja Intan M. W. Dewi |
author_sort |
Bayu A. P. Wilopo |
title |
Loop-Mediated Isothermal Amplification untuk Mendeteksi Gen blaTEM sebagai Penyandi Extended-Spectrum Beta-Lactamase pada Isolat Enterobacteriaceae |
title_short |
Loop-Mediated Isothermal Amplification untuk Mendeteksi Gen blaTEM sebagai Penyandi Extended-Spectrum Beta-Lactamase pada Isolat Enterobacteriaceae |
title_full |
Loop-Mediated Isothermal Amplification untuk Mendeteksi Gen blaTEM sebagai Penyandi Extended-Spectrum Beta-Lactamase pada Isolat Enterobacteriaceae |
title_fullStr |
Loop-Mediated Isothermal Amplification untuk Mendeteksi Gen blaTEM sebagai Penyandi Extended-Spectrum Beta-Lactamase pada Isolat Enterobacteriaceae |
title_full_unstemmed |
Loop-Mediated Isothermal Amplification untuk Mendeteksi Gen blaTEM sebagai Penyandi Extended-Spectrum Beta-Lactamase pada Isolat Enterobacteriaceae |
title_sort |
loop-mediated isothermal amplification untuk mendeteksi gen blatem sebagai penyandi extended-spectrum beta-lactamase pada isolat enterobacteriaceae |
publisher |
Universitas Padjajaran |
series |
Majalah Kedokteran Bandung |
issn |
0126-074X 2338-6223 |
publishDate |
2015-12-01 |
description |
Extended-spectrum beta-lactamase (ESBL) is a beta-lactamase enzyme that is capable of hydrolyzing penicillin, cephalosporin, and monobactam, and can be inhibited by clavulanic acid. This enzyme is encoded by multiple genes, one of them is blaTEM. Polymerase chain reaction (PCR) is one of the DNA amplification methods that are frequently used; however, there are other methods that can be used including, among others, loop-mediated isothermal amplification (LAMP). LAMP requires simple equipment with quicker and easy-to-read results compared to PCR. This study was a diagnostic test to explore the sensitivity and specificity of LAMP method compared to PCR in detecting blaTEM gene. Furthermore, the concordance between LAMP and PCR methods was assessed. A total of 92 Enterobacteriaceae isolates were examined by PCR and LAMP methods and compared. The result showed that the LAMP method had a sensitivity of 91.4% and a specificity of 91.2% with a concordance value (kappa) of 85.4%. In conclusion, LAMP method has a good validity and a very good conformity compared to the PCR method. Therefore, LAMP method can be used as an alternative diagnostic test, especially in limited settings. |
topic |
blaTEM Enterobacteriaceae ESBL LAMP PCR |
url |
http://journal.fk.unpad.ac.id/index.php/mkb/article/view/618 |
work_keys_str_mv |
AT bayuapwilopo loopmediatedisothermalamplificationuntukmendeteksigenblatemsebagaipenyandiextendedspectrumbetalactamasepadaisolatenterobacteriaceae AT sunarjatisudigdoadi loopmediatedisothermalamplificationuntukmendeteksigenblatemsebagaipenyandiextendedspectrumbetalactamasepadaisolatenterobacteriaceae AT edhyanasahiratmadja loopmediatedisothermalamplificationuntukmendeteksigenblatemsebagaipenyandiextendedspectrumbetalactamasepadaisolatenterobacteriaceae AT intanmwdewi loopmediatedisothermalamplificationuntukmendeteksigenblatemsebagaipenyandiextendedspectrumbetalactamasepadaisolatenterobacteriaceae |
_version_ |
1725682728322465792 |