Evaluation of Yersinia Enterocolitica Diagnostic Approaches
Background and Aim: Yersinia enterocolitica is a Gram-negative bacterium which its strains are involved in human diseases. To differentiate among pathogenic and non-pathogenic types, tests such as Congo Red absorption, Crystal Violet, and Calcium Dependency test are used. These tests are based on ex...
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Tehran University of Medical Sciences
2008-04-01
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doaj-3e7aca303626456c892646811a8359262021-10-02T01:16:54ZfasTehran University of Medical Sciencesپیاورد سلامت1735-81322008-26652008-04-0112510Evaluation of Yersinia Enterocolitica Diagnostic Approaches R BakhtiaryA BrahmehS JadidyS EshraghiF MatinMR KhorramizadehMM Soltan DallalF SaberpoorSZ Rouhani RankouhiBackground and Aim: Yersinia enterocolitica is a Gram-negative bacterium which its strains are involved in human diseases. To differentiate among pathogenic and non-pathogenic types, tests such as Congo Red absorption, Crystal Violet, and Calcium Dependency test are used. These tests are based on existence of 70-75 kb plasmids and sometimes, with respect to plasmids instability, we will face false negative results. Therefore, by setting up a methodology based on stable chromosomal genes of pathogenic agent we can overcome this hurdle. The goal of this survey was comparison among routine and molecular diagnostic approaches in the identification of Y. enterocolitica pathogenic strains.Materials and Methods: Some Gram-negative bacteria from family Enterobacteriacea and some Y. enterocolitica strains isolated of human beings and environment were evaluated.Results: Obtained results showed that 4 Y. enterocolitica strains isolated of human beings were PCR positive while PCR results of environmental strains, one human strain and non-Yersinia strains were negative.Conclusion: The mentioned approach can be used as a method to differentiate among pathogenic and non-pathogenic strains of Y. enterocolitica.http://journals.tums.ac.ir/PdfMed.aspx?pdf_med=/upload_files/pdf/12225.pdf&manuscript_id=12225Yersinia EnterocoliticaPathogenesisPCR |
collection |
DOAJ |
language |
fas |
format |
Article |
sources |
DOAJ |
author |
R Bakhtiary A Brahmeh S Jadidy S Eshraghi F Matin MR Khorramizadeh MM Soltan Dallal F Saberpoor SZ Rouhani Rankouhi |
spellingShingle |
R Bakhtiary A Brahmeh S Jadidy S Eshraghi F Matin MR Khorramizadeh MM Soltan Dallal F Saberpoor SZ Rouhani Rankouhi Evaluation of Yersinia Enterocolitica Diagnostic Approaches پیاورد سلامت Yersinia Enterocolitica Pathogenesis PCR |
author_facet |
R Bakhtiary A Brahmeh S Jadidy S Eshraghi F Matin MR Khorramizadeh MM Soltan Dallal F Saberpoor SZ Rouhani Rankouhi |
author_sort |
R Bakhtiary |
title |
Evaluation of Yersinia Enterocolitica Diagnostic Approaches |
title_short |
Evaluation of Yersinia Enterocolitica Diagnostic Approaches |
title_full |
Evaluation of Yersinia Enterocolitica Diagnostic Approaches |
title_fullStr |
Evaluation of Yersinia Enterocolitica Diagnostic Approaches |
title_full_unstemmed |
Evaluation of Yersinia Enterocolitica Diagnostic Approaches |
title_sort |
evaluation of yersinia enterocolitica diagnostic approaches |
publisher |
Tehran University of Medical Sciences |
series |
پیاورد سلامت |
issn |
1735-8132 2008-2665 |
publishDate |
2008-04-01 |
description |
Background and Aim: Yersinia enterocolitica is a Gram-negative bacterium which its strains are involved in human diseases. To differentiate among pathogenic and non-pathogenic types, tests such as Congo Red absorption, Crystal Violet, and Calcium Dependency test are used. These tests are based on existence of 70-75 kb plasmids and sometimes, with respect to plasmids instability, we will face false negative results. Therefore, by setting up a methodology based on stable chromosomal genes of pathogenic agent we can overcome this hurdle. The goal of this survey was comparison among routine and molecular diagnostic approaches in the identification of Y. enterocolitica pathogenic strains.Materials and Methods: Some Gram-negative bacteria from family Enterobacteriacea and some Y. enterocolitica strains isolated of human beings and environment were evaluated.Results: Obtained results showed that 4 Y. enterocolitica strains isolated of human beings were PCR positive while PCR results of environmental strains, one human strain and non-Yersinia strains were negative.Conclusion: The mentioned approach can be used as a method to differentiate among pathogenic and non-pathogenic strains of Y. enterocolitica. |
topic |
Yersinia Enterocolitica Pathogenesis PCR |
url |
http://journals.tums.ac.ir/PdfMed.aspx?pdf_med=/upload_files/pdf/12225.pdf&manuscript_id=12225 |
work_keys_str_mv |
AT rbakhtiary evaluationofyersiniaenterocoliticadiagnosticapproaches AT abrahmeh evaluationofyersiniaenterocoliticadiagnosticapproaches AT sjadidy evaluationofyersiniaenterocoliticadiagnosticapproaches AT seshraghi evaluationofyersiniaenterocoliticadiagnosticapproaches AT fmatin evaluationofyersiniaenterocoliticadiagnosticapproaches AT mrkhorramizadeh evaluationofyersiniaenterocoliticadiagnosticapproaches AT mmsoltandallal evaluationofyersiniaenterocoliticadiagnosticapproaches AT fsaberpoor evaluationofyersiniaenterocoliticadiagnosticapproaches AT szrouhanirankouhi evaluationofyersiniaenterocoliticadiagnosticapproaches |
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