Purified Gymnemic Acids from <i>Gymnema inodorum</i> Tea Inhibit 3T3-L1 Cell Differentiation into Adipocytes
<i>Gymnema inodorum</i> (GI) is an indigenous medicinal plant and functional food in Thailand that has recently helped to reduce plasma glucose levels in healthy humans. It is renowned for the medicinal properties of gymnemic acid and its ability to suppress glucose absorption. However,...
Main Authors: | , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
MDPI AG
2020-09-01
|
Series: | Nutrients |
Subjects: | |
Online Access: | https://www.mdpi.com/2072-6643/12/9/2851 |
id |
doaj-3e958b74a3184b31aff1440c0249a4dc |
---|---|
record_format |
Article |
spelling |
doaj-3e958b74a3184b31aff1440c0249a4dc2020-11-25T03:41:20ZengMDPI AGNutrients2072-66432020-09-01122851285110.3390/nu12092851Purified Gymnemic Acids from <i>Gymnema inodorum</i> Tea Inhibit 3T3-L1 Cell Differentiation into AdipocytesPapawee Saiki0Yasuhiro Kawano1Takayuki Ogi2Prapaipat Klungsupya3Thanchanok Muangman4Wimonsri Phantanaprates5Papitchaya Kongchinda6Nantaporn Pinnak7Koyomi Miyazaki8Cellular and Molecular Biotechnology Research Institute, National Institute of Advance Industrial Science and Technology (AIST), Tsukuba, Ibaraki 305-8566, JapanCellular and Molecular Biotechnology Research Institute, National Institute of Advance Industrial Science and Technology (AIST), Tsukuba, Ibaraki 305-8566, JapanDepartment of Environment and Natural Resources, Okinawa Industrial Technology Center, Okinawa 904-2234, JapanResearch and Development Group for Bio-Industries, Thailand Institute of Scientific and Technological Research (TISTR), Techno Polis, Khlong Luang, Pathum Thani 12120, ThailandResearch and Development Group for Bio-Industries, Thailand Institute of Scientific and Technological Research (TISTR), Techno Polis, Khlong Luang, Pathum Thani 12120, ThailandResearch and Development Group for Bio-Industries, Thailand Institute of Scientific and Technological Research (TISTR), Techno Polis, Khlong Luang, Pathum Thani 12120, ThailandResearch and Development Group for Bio-Industries, Thailand Institute of Scientific and Technological Research (TISTR), Techno Polis, Khlong Luang, Pathum Thani 12120, ThailandResearch and Development Group for Bio-Industries, Thailand Institute of Scientific and Technological Research (TISTR), Techno Polis, Khlong Luang, Pathum Thani 12120, ThailandCellular and Molecular Biotechnology Research Institute, National Institute of Advance Industrial Science and Technology (AIST), Tsukuba, Ibaraki 305-8566, Japan<i>Gymnema inodorum</i> (GI) is an indigenous medicinal plant and functional food in Thailand that has recently helped to reduce plasma glucose levels in healthy humans. It is renowned for the medicinal properties of gymnemic acid and its ability to suppress glucose absorption. However, the effects of gymnemic acids on adipogenesis that contribute to the accumulation of adipose tissues associated with obesity remain unknown. The present study aimed to determine the effects of gymnemic acids derived from GI tea on adipogenesis. We purified and identified GiA-7 and stephanosides C and B from GI tea that inhibited adipocyte differentiation in 3T3-L1 cells. These compounds also suppressed the expression of <i>peroxisome proliferator-activated receptor gamma</i> (<i>Pparγ</i>)-dependent genes, indicating that they inhibit lipid accumulation and the early stage of 3T3-L1 preadipocyte differentiation. Only GiA-7 induced the expression of <i>uncoupling protein 1</i> (<i>Ucp1</i>) and <i>pparγ coactivator 1 alpha</i> (<i>Pgc1α</i>), suggesting that GiA-7 induces mitochondrial activity and beige-like adipocytes. This is the first finding of stephanosides C and B in <i>Gymnema inodorum</i>. Our results suggested that GiA-7 and stephanosides C and B from GI tea could help to prevent obesity.https://www.mdpi.com/2072-6643/12/9/2851<i>Gymnema inodorum</i>adipogenesisgymnemic acidobesity |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Papawee Saiki Yasuhiro Kawano Takayuki Ogi Prapaipat Klungsupya Thanchanok Muangman Wimonsri Phantanaprates Papitchaya Kongchinda Nantaporn Pinnak Koyomi Miyazaki |
spellingShingle |
Papawee Saiki Yasuhiro Kawano Takayuki Ogi Prapaipat Klungsupya Thanchanok Muangman Wimonsri Phantanaprates Papitchaya Kongchinda Nantaporn Pinnak Koyomi Miyazaki Purified Gymnemic Acids from <i>Gymnema inodorum</i> Tea Inhibit 3T3-L1 Cell Differentiation into Adipocytes Nutrients <i>Gymnema inodorum</i> adipogenesis gymnemic acid obesity |
author_facet |
Papawee Saiki Yasuhiro Kawano Takayuki Ogi Prapaipat Klungsupya Thanchanok Muangman Wimonsri Phantanaprates Papitchaya Kongchinda Nantaporn Pinnak Koyomi Miyazaki |
author_sort |
Papawee Saiki |
title |
Purified Gymnemic Acids from <i>Gymnema inodorum</i> Tea Inhibit 3T3-L1 Cell Differentiation into Adipocytes |
title_short |
Purified Gymnemic Acids from <i>Gymnema inodorum</i> Tea Inhibit 3T3-L1 Cell Differentiation into Adipocytes |
title_full |
Purified Gymnemic Acids from <i>Gymnema inodorum</i> Tea Inhibit 3T3-L1 Cell Differentiation into Adipocytes |
title_fullStr |
Purified Gymnemic Acids from <i>Gymnema inodorum</i> Tea Inhibit 3T3-L1 Cell Differentiation into Adipocytes |
title_full_unstemmed |
Purified Gymnemic Acids from <i>Gymnema inodorum</i> Tea Inhibit 3T3-L1 Cell Differentiation into Adipocytes |
title_sort |
purified gymnemic acids from <i>gymnema inodorum</i> tea inhibit 3t3-l1 cell differentiation into adipocytes |
publisher |
MDPI AG |
series |
Nutrients |
issn |
2072-6643 |
publishDate |
2020-09-01 |
description |
<i>Gymnema inodorum</i> (GI) is an indigenous medicinal plant and functional food in Thailand that has recently helped to reduce plasma glucose levels in healthy humans. It is renowned for the medicinal properties of gymnemic acid and its ability to suppress glucose absorption. However, the effects of gymnemic acids on adipogenesis that contribute to the accumulation of adipose tissues associated with obesity remain unknown. The present study aimed to determine the effects of gymnemic acids derived from GI tea on adipogenesis. We purified and identified GiA-7 and stephanosides C and B from GI tea that inhibited adipocyte differentiation in 3T3-L1 cells. These compounds also suppressed the expression of <i>peroxisome proliferator-activated receptor gamma</i> (<i>Pparγ</i>)-dependent genes, indicating that they inhibit lipid accumulation and the early stage of 3T3-L1 preadipocyte differentiation. Only GiA-7 induced the expression of <i>uncoupling protein 1</i> (<i>Ucp1</i>) and <i>pparγ coactivator 1 alpha</i> (<i>Pgc1α</i>), suggesting that GiA-7 induces mitochondrial activity and beige-like adipocytes. This is the first finding of stephanosides C and B in <i>Gymnema inodorum</i>. Our results suggested that GiA-7 and stephanosides C and B from GI tea could help to prevent obesity. |
topic |
<i>Gymnema inodorum</i> adipogenesis gymnemic acid obesity |
url |
https://www.mdpi.com/2072-6643/12/9/2851 |
work_keys_str_mv |
AT papaweesaiki purifiedgymnemicacidsfromigymnemainodorumiteainhibit3t3l1celldifferentiationintoadipocytes AT yasuhirokawano purifiedgymnemicacidsfromigymnemainodorumiteainhibit3t3l1celldifferentiationintoadipocytes AT takayukiogi purifiedgymnemicacidsfromigymnemainodorumiteainhibit3t3l1celldifferentiationintoadipocytes AT prapaipatklungsupya purifiedgymnemicacidsfromigymnemainodorumiteainhibit3t3l1celldifferentiationintoadipocytes AT thanchanokmuangman purifiedgymnemicacidsfromigymnemainodorumiteainhibit3t3l1celldifferentiationintoadipocytes AT wimonsriphantanaprates purifiedgymnemicacidsfromigymnemainodorumiteainhibit3t3l1celldifferentiationintoadipocytes AT papitchayakongchinda purifiedgymnemicacidsfromigymnemainodorumiteainhibit3t3l1celldifferentiationintoadipocytes AT nantapornpinnak purifiedgymnemicacidsfromigymnemainodorumiteainhibit3t3l1celldifferentiationintoadipocytes AT koyomimiyazaki purifiedgymnemicacidsfromigymnemainodorumiteainhibit3t3l1celldifferentiationintoadipocytes |
_version_ |
1724530305508835328 |