Evaluation of umbilical cord mesenchymal stem cell labeling with superparamagnetic iron oxide nanoparticles coated with dextran and complexed with Poly-L-lysine

Objective: The objective of this study was to evaluate the effect of thelabeling of umbilical cord vein derived mesenchymal stem cells withsuperparamagnetic iron oxide nanoparticles coated with dextran andcomplexed to a non-viral transfector agent transfector poly-L-lysine.Methods: The labeling of m...

Full description

Bibliographic Details
Main Authors: Tatiana Taís Sibov, Liza Aya Mabuchi Miyaki, Javier Bustamante Mamani, Luciana Cavalheiro Marti, Luiz Roberto Sardinha, Lorena Favaro Pavon, Daniela Mara de Oliveira, Walter Humberto Cardenas, Lionel Fernel Gamarra
Format: Article
Language:English
Published: Instituto Israelita de Ensino e Pesquisa Albert Einstein 2012-06-01
Series:Einstein (São Paulo)
Subjects:
Online Access:http://apps.einstein.br/revista/arquivos/PDF/2251-180-188.pdf
id doaj-3eb2f62346cf4fd0805866dd08374ab2
record_format Article
spelling doaj-3eb2f62346cf4fd0805866dd08374ab22020-11-25T00:24:13ZengInstituto Israelita de Ensino e Pesquisa Albert EinsteinEinstein (São Paulo)1679-45082012-06-01102180188Evaluation of umbilical cord mesenchymal stem cell labeling with superparamagnetic iron oxide nanoparticles coated with dextran and complexed with Poly-L-lysineTatiana Taís SibovLiza Aya Mabuchi MiyakiJavier Bustamante MamaniLuciana Cavalheiro MartiLuiz Roberto SardinhaLorena Favaro PavonDaniela Mara de OliveiraWalter Humberto CardenasLionel Fernel GamarraObjective: The objective of this study was to evaluate the effect of thelabeling of umbilical cord vein derived mesenchymal stem cells withsuperparamagnetic iron oxide nanoparticles coated with dextran andcomplexed to a non-viral transfector agent transfector poly-L-lysine.Methods: The labeling of mesenchymal stem cells was performedusing the superparamagnetic iron oxide nanoparticles/dextrancomplexed and not complexed to poly-L-lysine. Superparamagneticiron oxide nanoparticles/dextran was incubated with poly-L-lysine inan ultrasonic sonicator at 37°C for 10 minutes for complex formationsuperparamagnetic iron oxide nanoparticles/dextran/poly-L-lysineby electrostatic interaction. Then, the mesenchymal stem cellswere incubated overnight with the complex superparamagnetic ironoxide nanoparticles/dextran/poly-L-lysine and superparamagneticiron oxide nanoparticles/dextran. After the incubation period themesenchymal stem cells were evaluated by internalization of thecomplex superparamagnetic iron oxide nanoparticles/dextran/poly-L-lysine and superparamagnetic iron oxide nanoparticles/dextran byPrussian Blue stain. Cellular viability of labeled mesenchymal stemcells was evaluated by cellular proliferation assay using 5,6-carboxyfluorescein-succinimidyl ester method and apoptosis detectionby Annexin V- Propidium Iodide assay. Results: mesenchymalstem cells labeled with superparamagnetic iron oxide nanoparticles/dextran without poly-L-lysine not internalized efficiently thesuperparamagnetic iron oxide nanoparticles due to its low presencedetected within cells. Mesenchymal stem cells labeled with thecomplex superparamagnetic iron oxide nanoparticles/dextran/poly-L-lysine efficiently internalized the superparamagnetic iron oxidenanoparticles due to greater presence in the cells interior. The viabilityand apoptosis assays demonstrated that the mesenchymal stemcells labeled and not labeled respectively with the superparamagneticiron oxide nanoparticles/dextran/poly-L-lysine continue to proliferateover seven days and the percentage of cells in early or late apoptosisis low compared to the percentage of live cells over the threedays. Conclusion: Our results showed that the use of poly-L-lysinecomplexed with superparamagnetic iron oxide nanoparticles/dextranprovides better internalization of these superparamagnetic iron oxidenanoparticles in mesenchymal stem cells Thus, we demonstratedthat this type of labeling is not cytotoxic to the mesenchymal stemcells, since the viability and apoptosis assays showed that the cellsremain alive and proliferating. The efficiency of this type of labelingin mesenchymal stem cells can provide non-invasive methods formonitoring these cells in vivohttp://apps.einstein.br/revista/arquivos/PDF/2251-180-188.pdfMesenchymal stem cellsMagnetite nanoparticlesPoly- L-LysineUmbilical veins
collection DOAJ
language English
format Article
sources DOAJ
author Tatiana Taís Sibov
Liza Aya Mabuchi Miyaki
Javier Bustamante Mamani
Luciana Cavalheiro Marti
Luiz Roberto Sardinha
Lorena Favaro Pavon
Daniela Mara de Oliveira
Walter Humberto Cardenas
Lionel Fernel Gamarra
spellingShingle Tatiana Taís Sibov
Liza Aya Mabuchi Miyaki
Javier Bustamante Mamani
Luciana Cavalheiro Marti
Luiz Roberto Sardinha
Lorena Favaro Pavon
Daniela Mara de Oliveira
Walter Humberto Cardenas
Lionel Fernel Gamarra
Evaluation of umbilical cord mesenchymal stem cell labeling with superparamagnetic iron oxide nanoparticles coated with dextran and complexed with Poly-L-lysine
Einstein (São Paulo)
Mesenchymal stem cells
Magnetite nanoparticles
Poly- L-Lysine
Umbilical veins
author_facet Tatiana Taís Sibov
Liza Aya Mabuchi Miyaki
Javier Bustamante Mamani
Luciana Cavalheiro Marti
Luiz Roberto Sardinha
Lorena Favaro Pavon
Daniela Mara de Oliveira
Walter Humberto Cardenas
Lionel Fernel Gamarra
author_sort Tatiana Taís Sibov
title Evaluation of umbilical cord mesenchymal stem cell labeling with superparamagnetic iron oxide nanoparticles coated with dextran and complexed with Poly-L-lysine
title_short Evaluation of umbilical cord mesenchymal stem cell labeling with superparamagnetic iron oxide nanoparticles coated with dextran and complexed with Poly-L-lysine
title_full Evaluation of umbilical cord mesenchymal stem cell labeling with superparamagnetic iron oxide nanoparticles coated with dextran and complexed with Poly-L-lysine
title_fullStr Evaluation of umbilical cord mesenchymal stem cell labeling with superparamagnetic iron oxide nanoparticles coated with dextran and complexed with Poly-L-lysine
title_full_unstemmed Evaluation of umbilical cord mesenchymal stem cell labeling with superparamagnetic iron oxide nanoparticles coated with dextran and complexed with Poly-L-lysine
title_sort evaluation of umbilical cord mesenchymal stem cell labeling with superparamagnetic iron oxide nanoparticles coated with dextran and complexed with poly-l-lysine
publisher Instituto Israelita de Ensino e Pesquisa Albert Einstein
series Einstein (São Paulo)
issn 1679-4508
publishDate 2012-06-01
description Objective: The objective of this study was to evaluate the effect of thelabeling of umbilical cord vein derived mesenchymal stem cells withsuperparamagnetic iron oxide nanoparticles coated with dextran andcomplexed to a non-viral transfector agent transfector poly-L-lysine.Methods: The labeling of mesenchymal stem cells was performedusing the superparamagnetic iron oxide nanoparticles/dextrancomplexed and not complexed to poly-L-lysine. Superparamagneticiron oxide nanoparticles/dextran was incubated with poly-L-lysine inan ultrasonic sonicator at 37°C for 10 minutes for complex formationsuperparamagnetic iron oxide nanoparticles/dextran/poly-L-lysineby electrostatic interaction. Then, the mesenchymal stem cellswere incubated overnight with the complex superparamagnetic ironoxide nanoparticles/dextran/poly-L-lysine and superparamagneticiron oxide nanoparticles/dextran. After the incubation period themesenchymal stem cells were evaluated by internalization of thecomplex superparamagnetic iron oxide nanoparticles/dextran/poly-L-lysine and superparamagnetic iron oxide nanoparticles/dextran byPrussian Blue stain. Cellular viability of labeled mesenchymal stemcells was evaluated by cellular proliferation assay using 5,6-carboxyfluorescein-succinimidyl ester method and apoptosis detectionby Annexin V- Propidium Iodide assay. Results: mesenchymalstem cells labeled with superparamagnetic iron oxide nanoparticles/dextran without poly-L-lysine not internalized efficiently thesuperparamagnetic iron oxide nanoparticles due to its low presencedetected within cells. Mesenchymal stem cells labeled with thecomplex superparamagnetic iron oxide nanoparticles/dextran/poly-L-lysine efficiently internalized the superparamagnetic iron oxidenanoparticles due to greater presence in the cells interior. The viabilityand apoptosis assays demonstrated that the mesenchymal stemcells labeled and not labeled respectively with the superparamagneticiron oxide nanoparticles/dextran/poly-L-lysine continue to proliferateover seven days and the percentage of cells in early or late apoptosisis low compared to the percentage of live cells over the threedays. Conclusion: Our results showed that the use of poly-L-lysinecomplexed with superparamagnetic iron oxide nanoparticles/dextranprovides better internalization of these superparamagnetic iron oxidenanoparticles in mesenchymal stem cells Thus, we demonstratedthat this type of labeling is not cytotoxic to the mesenchymal stemcells, since the viability and apoptosis assays showed that the cellsremain alive and proliferating. The efficiency of this type of labelingin mesenchymal stem cells can provide non-invasive methods formonitoring these cells in vivo
topic Mesenchymal stem cells
Magnetite nanoparticles
Poly- L-Lysine
Umbilical veins
url http://apps.einstein.br/revista/arquivos/PDF/2251-180-188.pdf
work_keys_str_mv AT tatianataissibov evaluationofumbilicalcordmesenchymalstemcelllabelingwithsuperparamagneticironoxidenanoparticlescoatedwithdextranandcomplexedwithpolyllysine
AT lizaayamabuchimiyaki evaluationofumbilicalcordmesenchymalstemcelllabelingwithsuperparamagneticironoxidenanoparticlescoatedwithdextranandcomplexedwithpolyllysine
AT javierbustamantemamani evaluationofumbilicalcordmesenchymalstemcelllabelingwithsuperparamagneticironoxidenanoparticlescoatedwithdextranandcomplexedwithpolyllysine
AT lucianacavalheiromarti evaluationofumbilicalcordmesenchymalstemcelllabelingwithsuperparamagneticironoxidenanoparticlescoatedwithdextranandcomplexedwithpolyllysine
AT luizrobertosardinha evaluationofumbilicalcordmesenchymalstemcelllabelingwithsuperparamagneticironoxidenanoparticlescoatedwithdextranandcomplexedwithpolyllysine
AT lorenafavaropavon evaluationofumbilicalcordmesenchymalstemcelllabelingwithsuperparamagneticironoxidenanoparticlescoatedwithdextranandcomplexedwithpolyllysine
AT danielamaradeoliveira evaluationofumbilicalcordmesenchymalstemcelllabelingwithsuperparamagneticironoxidenanoparticlescoatedwithdextranandcomplexedwithpolyllysine
AT walterhumbertocardenas evaluationofumbilicalcordmesenchymalstemcelllabelingwithsuperparamagneticironoxidenanoparticlescoatedwithdextranandcomplexedwithpolyllysine
AT lionelfernelgamarra evaluationofumbilicalcordmesenchymalstemcelllabelingwithsuperparamagneticironoxidenanoparticlescoatedwithdextranandcomplexedwithpolyllysine
_version_ 1725353260140724224