Extra Virgin Olive Oil Phenols Vasodilate Rat MesentericResistance Artery via Phospholipase C (PLC)-CalciumMicrodomains-Potassium Channels (BK<sub>Ca</sub>) Signals
Recent evidence suggests that the reason Extra Virgin Olive Oil (EVOO) lowers blood pressure and reduces the risk of developing hypertension is partly due to minor components of EVOO, such as phenols. However, little is still known about the mechanism(s) through which EVOO phenols mediate anti-hyper...
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doaj-3ed3e259db1d4289b68429ebd0b8751e2021-01-22T00:06:02ZengMDPI AGBiomolecules2218-273X2021-01-011113713710.3390/biom11020137Extra Virgin Olive Oil Phenols Vasodilate Rat MesentericResistance Artery via Phospholipase C (PLC)-CalciumMicrodomains-Potassium Channels (BK<sub>Ca</sub>) SignalsRossana D’Agostino0Laura Barberio1Mariacarmela Gatto2Teresa Tropea3Maria De Luca4Maurizio Mandalà5Department of Biology, Ecology and Earth Sciences, University of Calabria, 87036 Rende, ItalyDepartment of Biology, Ecology and Earth Sciences, University of Calabria, 87036 Rende, ItalyDepartment of Biology, Ecology and Earth Sciences, University of Calabria, 87036 Rende, ItalyDepartment of Biology, Ecology and Earth Sciences, University of Calabria, 87036 Rende, ItalyDepartment of Nutrition Sciences, University of Alabama at Birmingham, Birmingham, AL 35294, USADepartment of Biology, Ecology and Earth Sciences, University of Calabria, 87036 Rende, ItalyRecent evidence suggests that the reason Extra Virgin Olive Oil (EVOO) lowers blood pressure and reduces the risk of developing hypertension is partly due to minor components of EVOO, such as phenols. However, little is still known about the mechanism(s) through which EVOO phenols mediate anti-hypertensive effects. The aim of the present study was to investigate the mechanisms of action of EVOO phenols on mesenteric resistance arteries. A pressure myograph was used to test the effect of EVOO phenols on isolated mesenteric arteries in the presence of specific inhibitors of: 1) BKca channels (Paxillin, 10<sup>−5</sup> M); 2) L-type calcium channels (Verapamil, 10<sup>−5</sup> M); 3) Ryanodine receptor, RyR (Ryanodine, 10<sup>−5</sup> M); 4) inositol 1,4,5-triphosphate receptor, IP3R, (2-Aminoethyl diphenylborinate, 2-APB, 3 × 10<sup>−3</sup> M); 5) phospholipase C, PLC, (U73122, 10<sup>−5</sup> M), and 6) GPCR-G<sub>αi</sub> signaling, (Pertussis Toxin, 10<sup>−5</sup> M). EVOO phenols induced vasodilation of mesenteric arteries in a dose-dependent manner, and this effect was reduced by pre-incubation with Paxillin, Verapamil, Ryanodine, 2-APB, U73122, and Pertussis Toxin. Our data suggest that EVOO phenol-mediated vasodilation requires activation of BKca channels potentially through a local increase of subcellular calcium microdomains, a pivotal mechanism on the base of artery vasodilation. These findings provide novel mechanistic insights for understanding the vasodilatory properties of EVOO phenols on resistance arteries.https://www.mdpi.com/2218-273X/11/2/137mesenteric arteryBK<sub>Ca</sub> channelsCa<sup>2+</sup> microdomainsPLCGPCR-Gαi |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Rossana D’Agostino Laura Barberio Mariacarmela Gatto Teresa Tropea Maria De Luca Maurizio Mandalà |
spellingShingle |
Rossana D’Agostino Laura Barberio Mariacarmela Gatto Teresa Tropea Maria De Luca Maurizio Mandalà Extra Virgin Olive Oil Phenols Vasodilate Rat MesentericResistance Artery via Phospholipase C (PLC)-CalciumMicrodomains-Potassium Channels (BK<sub>Ca</sub>) Signals Biomolecules mesenteric artery BK<sub>Ca</sub> channels Ca<sup>2+</sup> microdomains PLC GPCR-Gαi |
author_facet |
Rossana D’Agostino Laura Barberio Mariacarmela Gatto Teresa Tropea Maria De Luca Maurizio Mandalà |
author_sort |
Rossana D’Agostino |
title |
Extra Virgin Olive Oil Phenols Vasodilate Rat MesentericResistance Artery via Phospholipase C (PLC)-CalciumMicrodomains-Potassium Channels (BK<sub>Ca</sub>) Signals |
title_short |
Extra Virgin Olive Oil Phenols Vasodilate Rat MesentericResistance Artery via Phospholipase C (PLC)-CalciumMicrodomains-Potassium Channels (BK<sub>Ca</sub>) Signals |
title_full |
Extra Virgin Olive Oil Phenols Vasodilate Rat MesentericResistance Artery via Phospholipase C (PLC)-CalciumMicrodomains-Potassium Channels (BK<sub>Ca</sub>) Signals |
title_fullStr |
Extra Virgin Olive Oil Phenols Vasodilate Rat MesentericResistance Artery via Phospholipase C (PLC)-CalciumMicrodomains-Potassium Channels (BK<sub>Ca</sub>) Signals |
title_full_unstemmed |
Extra Virgin Olive Oil Phenols Vasodilate Rat MesentericResistance Artery via Phospholipase C (PLC)-CalciumMicrodomains-Potassium Channels (BK<sub>Ca</sub>) Signals |
title_sort |
extra virgin olive oil phenols vasodilate rat mesentericresistance artery via phospholipase c (plc)-calciummicrodomains-potassium channels (bk<sub>ca</sub>) signals |
publisher |
MDPI AG |
series |
Biomolecules |
issn |
2218-273X |
publishDate |
2021-01-01 |
description |
Recent evidence suggests that the reason Extra Virgin Olive Oil (EVOO) lowers blood pressure and reduces the risk of developing hypertension is partly due to minor components of EVOO, such as phenols. However, little is still known about the mechanism(s) through which EVOO phenols mediate anti-hypertensive effects. The aim of the present study was to investigate the mechanisms of action of EVOO phenols on mesenteric resistance arteries. A pressure myograph was used to test the effect of EVOO phenols on isolated mesenteric arteries in the presence of specific inhibitors of: 1) BKca channels (Paxillin, 10<sup>−5</sup> M); 2) L-type calcium channels (Verapamil, 10<sup>−5</sup> M); 3) Ryanodine receptor, RyR (Ryanodine, 10<sup>−5</sup> M); 4) inositol 1,4,5-triphosphate receptor, IP3R, (2-Aminoethyl diphenylborinate, 2-APB, 3 × 10<sup>−3</sup> M); 5) phospholipase C, PLC, (U73122, 10<sup>−5</sup> M), and 6) GPCR-G<sub>αi</sub> signaling, (Pertussis Toxin, 10<sup>−5</sup> M). EVOO phenols induced vasodilation of mesenteric arteries in a dose-dependent manner, and this effect was reduced by pre-incubation with Paxillin, Verapamil, Ryanodine, 2-APB, U73122, and Pertussis Toxin. Our data suggest that EVOO phenol-mediated vasodilation requires activation of BKca channels potentially through a local increase of subcellular calcium microdomains, a pivotal mechanism on the base of artery vasodilation. These findings provide novel mechanistic insights for understanding the vasodilatory properties of EVOO phenols on resistance arteries. |
topic |
mesenteric artery BK<sub>Ca</sub> channels Ca<sup>2+</sup> microdomains PLC GPCR-Gαi |
url |
https://www.mdpi.com/2218-273X/11/2/137 |
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