A Comparison of Microscopy and Enzyme Linked Immunosorbent Assay for Diagnosis of Giardia lamblia in Human Faecal Specimens
Background: Giardia lamblia, a flagellate protozoa, is a common causative agent of parasitic diarrhoeal diseases of humans. Laboratory diagnosis mainly consists of direct microscopic examination of stool specimen for trophozoite and cysts of Giardia. However, due to intermittent faecal excretion...
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doaj-3ed6aaf026b6411e9c000215f0603e0c2020-11-25T03:26:56ZengJCDR Research and Publications Private LimitedJournal of Clinical and Diagnostic Research2249-782X0973-709X2014-11-01811DC04DC0610.7860/JCDR/2014/9484.5087A Comparison of Microscopy and Enzyme Linked Immunosorbent Assay for Diagnosis of Giardia lamblia in Human Faecal SpecimensNoor Jahan0Razia Khatoon1Siraj Ahmad2Assistant Professor, Department of Microbiology, Era’s Lucknow Medical College and Hospital, Lucknow, India.Assistant Professor, Department of Microbiology, Era’s Lucknow Medical College and Hospital, Lucknow, India.Associate Professor, Department of Community Medicine, Teerthanker Mahaveer Medical College and Research Centre, Teerthanker Mahaveer University, Moradabad, India.Background: Giardia lamblia, a flagellate protozoa, is a common causative agent of parasitic diarrhoeal diseases of humans. Laboratory diagnosis mainly consists of direct microscopic examination of stool specimen for trophozoite and cysts of Giardia. However, due to intermittent faecal excretion of parasite, the case may be miss diagnosed and the patient may continue excreting the parasite and infecting others. Therefore, other mode of diagnosis should be looked for, which overcome the above drawbacks of microscopy used alone for diagnosis. Objectives: The present study was done to evaluate the efficacy of RIDASCREEN Giardia (ELISA) test in comparison to direct microscopy in the diagnosis of Giardia lamblia in stool specimens from patients with diarrhea and other gastrointestinal symptoms. Materials and Methods: A total of 1680 patients were included in the study and three faecal specimens were taken from each patient which was divided into two parts. One part was used for direct wet mount examination and second part was used to put ELISA by using RIDASCREEN Giardia test. Results: Out of 1680 stool samples, 380 specimens (22.6%) were found to be positive for Giardia lamblia. Maximum cases were detected by RIDASCREEN Giardia (ELISA) test with sensitivity of 100% and specificity of 91.5%. Maximum cases of giardiasis were detected in children less than 10 y of age (12.8%). Conclusion: RIDASCREEN Giardia test is a rapid and effective method with high sensitivity and specificity and detects Giardia antigens in stool specimens even when the count of parasite is low, thus reducing the chances of missing even the asymptomatic cases.https://jcdr.net/articles/PDF/5087/9484_CE(Ra)_F(Sh)_PF1(AJAK)_PFA(Sh)_PF2(PAG).pdfdirect wet mount microscopygiardia lambliaridascreen giardia teststool concentration |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Noor Jahan Razia Khatoon Siraj Ahmad |
spellingShingle |
Noor Jahan Razia Khatoon Siraj Ahmad A Comparison of Microscopy and Enzyme Linked Immunosorbent Assay for Diagnosis of Giardia lamblia in Human Faecal Specimens Journal of Clinical and Diagnostic Research direct wet mount microscopy giardia lamblia ridascreen giardia test stool concentration |
author_facet |
Noor Jahan Razia Khatoon Siraj Ahmad |
author_sort |
Noor Jahan |
title |
A Comparison of Microscopy and Enzyme Linked Immunosorbent Assay for Diagnosis of Giardia lamblia in Human Faecal Specimens |
title_short |
A Comparison of Microscopy and Enzyme Linked Immunosorbent Assay for Diagnosis of Giardia lamblia in Human Faecal Specimens |
title_full |
A Comparison of Microscopy and Enzyme Linked Immunosorbent Assay for Diagnosis of Giardia lamblia in Human Faecal Specimens |
title_fullStr |
A Comparison of Microscopy and Enzyme Linked Immunosorbent Assay for Diagnosis of Giardia lamblia in Human Faecal Specimens |
title_full_unstemmed |
A Comparison of Microscopy and Enzyme Linked Immunosorbent Assay for Diagnosis of Giardia lamblia in Human Faecal Specimens |
title_sort |
comparison of microscopy and enzyme linked immunosorbent assay for diagnosis of giardia lamblia in human faecal specimens |
publisher |
JCDR Research and Publications Private Limited |
series |
Journal of Clinical and Diagnostic Research |
issn |
2249-782X 0973-709X |
publishDate |
2014-11-01 |
description |
Background: Giardia lamblia, a flagellate protozoa, is a common
causative agent of parasitic diarrhoeal diseases of humans.
Laboratory diagnosis mainly consists of direct microscopic
examination of stool specimen for trophozoite and cysts
of Giardia. However, due to intermittent faecal excretion of
parasite, the case may be miss diagnosed and the patient may
continue excreting the parasite and infecting others. Therefore,
other mode of diagnosis should be looked for, which overcome
the above drawbacks of microscopy used alone for diagnosis.
Objectives: The present study was done to evaluate the
efficacy of RIDASCREEN Giardia (ELISA) test in comparison to
direct microscopy in the diagnosis of Giardia lamblia in stool
specimens from patients with diarrhea and other gastrointestinal
symptoms.
Materials and Methods: A total of 1680 patients were included
in the study and three faecal specimens were taken from each
patient which was divided into two parts. One part was used for
direct wet mount examination and second part was used to put
ELISA by using RIDASCREEN Giardia test.
Results: Out of 1680 stool samples, 380 specimens (22.6%)
were found to be positive for Giardia lamblia. Maximum cases
were detected by RIDASCREEN Giardia (ELISA) test with
sensitivity of 100% and specificity of 91.5%. Maximum cases
of giardiasis were detected in children less than 10 y of age
(12.8%).
Conclusion: RIDASCREEN Giardia test is a rapid and effective
method with high sensitivity and specificity and detects
Giardia antigens in stool specimens even when the count of
parasite is low, thus reducing the chances of missing even the
asymptomatic cases. |
topic |
direct wet mount microscopy giardia lamblia ridascreen giardia test stool concentration |
url |
https://jcdr.net/articles/PDF/5087/9484_CE(Ra)_F(Sh)_PF1(AJAK)_PFA(Sh)_PF2(PAG).pdf |
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