Human Pirh2 is A Novel Inhibitor of Prototype Foamy Virus Replication
Prototype foamy virus (PFV) is a member of the unconventional and nonpathogenic retroviruses. PFV causes lifelong chronic infections, which are partially attributable to a number of host cell factors that restrict viral replication. Herein, we identified human p53-induced RING-H2 protein (Pirh2) as...
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doaj-3eef7b3b3c8142538c25ad07139ebd3d2020-11-24T21:15:55ZengMDPI AGViruses1999-49152015-04-01741668168410.3390/v7041668v7041668Human Pirh2 is A Novel Inhibitor of Prototype Foamy Virus ReplicationLanlan Dong0Qingqing Cheng1Zhihao Wang2Peipei Yuan3Zhi Li4Yan Sun5Song Han6Jun Yin7Biwen Peng8Xiaohua He9Wanhong Liu10Pathogenic Organism and Infectious Diseases Research Institute, School of Basic Medical Sciences, Wuhan University, Donghu Road No. 185, Wuchang, Wuhan 430071, ChinaPathogenic Organism and Infectious Diseases Research Institute, School of Basic Medical Sciences, Wuhan University, Donghu Road No. 185, Wuchang, Wuhan 430071, ChinaPathogenic Organism and Infectious Diseases Research Institute, School of Basic Medical Sciences, Wuhan University, Donghu Road No. 185, Wuchang, Wuhan 430071, ChinaPathogenic Organism and Infectious Diseases Research Institute, School of Basic Medical Sciences, Wuhan University, Donghu Road No. 185, Wuchang, Wuhan 430071, ChinaCollege of Life Sciences, Shanxi Normal University, Xi'an 710062, ChinaCollege of Life Sciences, Shanxi Normal University, Xi'an 710062, ChinaPathogenic Organism and Infectious Diseases Research Institute, School of Basic Medical Sciences, Wuhan University, Donghu Road No. 185, Wuchang, Wuhan 430071, ChinaPathogenic Organism and Infectious Diseases Research Institute, School of Basic Medical Sciences, Wuhan University, Donghu Road No. 185, Wuchang, Wuhan 430071, ChinaPathogenic Organism and Infectious Diseases Research Institute, School of Basic Medical Sciences, Wuhan University, Donghu Road No. 185, Wuchang, Wuhan 430071, ChinaPathogenic Organism and Infectious Diseases Research Institute, School of Basic Medical Sciences, Wuhan University, Donghu Road No. 185, Wuchang, Wuhan 430071, ChinaPathogenic Organism and Infectious Diseases Research Institute, School of Basic Medical Sciences, Wuhan University, Donghu Road No. 185, Wuchang, Wuhan 430071, ChinaPrototype foamy virus (PFV) is a member of the unconventional and nonpathogenic retroviruses. PFV causes lifelong chronic infections, which are partially attributable to a number of host cell factors that restrict viral replication. Herein, we identified human p53-induced RING-H2 protein (Pirh2) as a novel inhibitor of prototype foamy virus. Overexpression of Pirh2 decreased the replication of PFV, whereas knockdown of Pirh2 with specific siRNA increased PFV replication. Dual-luciferase assays and coimmunoprecipitation demonstrated that Pirh2 negatively influences the Tas-dependent transcriptional activation of the PFV long terminal repeat (LTR) and internal promoter (IP) by interacting with the transactivator Tas and down-regulating its expression. In addition, the viral inhibitory function of Pirh2 is N-terminal and RING domain dependent. Together, these results indicated that Pirh2 suppresses PFV replication by negatively impacting its transactivator Tas and the transcription of two viral promoters, which may contribute to the latency of PFV infection.http://www.mdpi.com/1999-4915/7/4/1668PFVPirh2replicationubiquitination |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Lanlan Dong Qingqing Cheng Zhihao Wang Peipei Yuan Zhi Li Yan Sun Song Han Jun Yin Biwen Peng Xiaohua He Wanhong Liu |
spellingShingle |
Lanlan Dong Qingqing Cheng Zhihao Wang Peipei Yuan Zhi Li Yan Sun Song Han Jun Yin Biwen Peng Xiaohua He Wanhong Liu Human Pirh2 is A Novel Inhibitor of Prototype Foamy Virus Replication Viruses PFV Pirh2 replication ubiquitination |
author_facet |
Lanlan Dong Qingqing Cheng Zhihao Wang Peipei Yuan Zhi Li Yan Sun Song Han Jun Yin Biwen Peng Xiaohua He Wanhong Liu |
author_sort |
Lanlan Dong |
title |
Human Pirh2 is A Novel Inhibitor of Prototype Foamy Virus Replication |
title_short |
Human Pirh2 is A Novel Inhibitor of Prototype Foamy Virus Replication |
title_full |
Human Pirh2 is A Novel Inhibitor of Prototype Foamy Virus Replication |
title_fullStr |
Human Pirh2 is A Novel Inhibitor of Prototype Foamy Virus Replication |
title_full_unstemmed |
Human Pirh2 is A Novel Inhibitor of Prototype Foamy Virus Replication |
title_sort |
human pirh2 is a novel inhibitor of prototype foamy virus replication |
publisher |
MDPI AG |
series |
Viruses |
issn |
1999-4915 |
publishDate |
2015-04-01 |
description |
Prototype foamy virus (PFV) is a member of the unconventional and nonpathogenic retroviruses. PFV causes lifelong chronic infections, which are partially attributable to a number of host cell factors that restrict viral replication. Herein, we identified human p53-induced RING-H2 protein (Pirh2) as a novel inhibitor of prototype foamy virus. Overexpression of Pirh2 decreased the replication of PFV, whereas knockdown of Pirh2 with specific siRNA increased PFV replication. Dual-luciferase assays and coimmunoprecipitation demonstrated that Pirh2 negatively influences the Tas-dependent transcriptional activation of the PFV long terminal repeat (LTR) and internal promoter (IP) by interacting with the transactivator Tas and down-regulating its expression. In addition, the viral inhibitory function of Pirh2 is N-terminal and RING domain dependent. Together, these results indicated that Pirh2 suppresses PFV replication by negatively impacting its transactivator Tas and the transcription of two viral promoters, which may contribute to the latency of PFV infection. |
topic |
PFV Pirh2 replication ubiquitination |
url |
http://www.mdpi.com/1999-4915/7/4/1668 |
work_keys_str_mv |
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