Expression of Musashi-1 Increases in Bone Healing
Musashi-1 (MSI1) is an RNA-binding protein that regulates progenitor cells in adult and developing organisms to maintain self-renewal capacities. The role of musashi-1 in the bone healing environment and its relation with other osteogenic factors is unknown. In the current study, we analyze the expr...
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doaj-3efcb27cd2e3431ba08c3aaad84113982021-03-27T00:02:28ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-03-01223395339510.3390/ijms22073395Expression of Musashi-1 Increases in Bone HealingMiguel Padial-Molina0Vicente Crespo-Lora1Clara Candido-Corral2Nati Martin-Morales3Dario Abril-Garcia4Pablo Galindo-Moreno5Pedro Hernandez-Cortes6Francisco O’Valle7Department of Oral Surgery and Implant Dentistry, School of Dentistry, and Centre for Biomedical Research (CIBM), University of Granada, 18071 Granada, SpainDepartment of Pathology, Institute of Biopathology and Regenerative Medicine (IBIMER, CIBM), Institute of Biosanitary (ibs-Granada), University of Granada, 18071 Granada, SpainDepartment of Pathology, Institute of Biopathology and Regenerative Medicine (IBIMER, CIBM), Institute of Biosanitary (ibs-Granada), University of Granada, 18071 Granada, SpainDepartment of Oral Surgery and Implant Dentistry, School of Dentistry, and Centre for Biomedical Research (CIBM), University of Granada, 18071 Granada, SpainDepartment of Oral Surgery and Implant Dentistry, School of Dentistry, and Centre for Biomedical Research (CIBM), University of Granada, 18071 Granada, SpainDepartment of Oral Surgery and Implant Dentistry, School of Dentistry, and Centre for Biomedical Research (CIBM), University of Granada, 18071 Granada, SpainDepartment of Orthopedic Surgery, San Cecilio University Hospital, 18071 Granada, SpainDepartment of Pathology, Institute of Biopathology and Regenerative Medicine (IBIMER, CIBM), Institute of Biosanitary (ibs-Granada), University of Granada, 18071 Granada, SpainMusashi-1 (MSI1) is an RNA-binding protein that regulates progenitor cells in adult and developing organisms to maintain self-renewal capacities. The role of musashi-1 in the bone healing environment and its relation with other osteogenic factors is unknown. In the current study, we analyze the expression of MSI1 in an experimental model of rat femoral bone fractures. We also analyze the relation between MSI1 expression and the expression of two osteogenic markers: periostin (POSTN) and runt-related transcription factor 2 (RUNX2). We use histological, immunohistochemical, and qPCR techniques to evaluate bone healing and the expression of MSI1, POSTN, and RUNX2 over time (4, 7, and 14 days). We compare our findings with non-fractured controls. We find that in bone calluses, the number of cells expressing MSI1 and RUNX2 increase over time and the intensity of POSTN expression decreases over time. Within bone calluses, we find the presence of MSI1 expression in mesenchymal stromal cells, osteoblasts, and osteocytes but not in hypertrophic chondrocytes. After 14 days, the expression of MSI1, POSTN, and RUNX2 was significantly correlated. Thus, we conclude that musashi-1 potentially serves in the osteogenic differentiation of mesenchymal stromal cells and bone healing. Therefore, further studies are needed to determine the possibility of musashi-1<inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><msup><mrow></mrow><mo>′</mo></msup></semantics></math></inline-formula>s role as a clinical biomarker of bone healing and therapeutic agent for bone regeneration.https://www.mdpi.com/1422-0067/22/7/3395Musashi-1Runx2periostinmesenchymal stem cellsbone healing |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Miguel Padial-Molina Vicente Crespo-Lora Clara Candido-Corral Nati Martin-Morales Dario Abril-Garcia Pablo Galindo-Moreno Pedro Hernandez-Cortes Francisco O’Valle |
spellingShingle |
Miguel Padial-Molina Vicente Crespo-Lora Clara Candido-Corral Nati Martin-Morales Dario Abril-Garcia Pablo Galindo-Moreno Pedro Hernandez-Cortes Francisco O’Valle Expression of Musashi-1 Increases in Bone Healing International Journal of Molecular Sciences Musashi-1 Runx2 periostin mesenchymal stem cells bone healing |
author_facet |
Miguel Padial-Molina Vicente Crespo-Lora Clara Candido-Corral Nati Martin-Morales Dario Abril-Garcia Pablo Galindo-Moreno Pedro Hernandez-Cortes Francisco O’Valle |
author_sort |
Miguel Padial-Molina |
title |
Expression of Musashi-1 Increases in Bone Healing |
title_short |
Expression of Musashi-1 Increases in Bone Healing |
title_full |
Expression of Musashi-1 Increases in Bone Healing |
title_fullStr |
Expression of Musashi-1 Increases in Bone Healing |
title_full_unstemmed |
Expression of Musashi-1 Increases in Bone Healing |
title_sort |
expression of musashi-1 increases in bone healing |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1661-6596 1422-0067 |
publishDate |
2021-03-01 |
description |
Musashi-1 (MSI1) is an RNA-binding protein that regulates progenitor cells in adult and developing organisms to maintain self-renewal capacities. The role of musashi-1 in the bone healing environment and its relation with other osteogenic factors is unknown. In the current study, we analyze the expression of MSI1 in an experimental model of rat femoral bone fractures. We also analyze the relation between MSI1 expression and the expression of two osteogenic markers: periostin (POSTN) and runt-related transcription factor 2 (RUNX2). We use histological, immunohistochemical, and qPCR techniques to evaluate bone healing and the expression of MSI1, POSTN, and RUNX2 over time (4, 7, and 14 days). We compare our findings with non-fractured controls. We find that in bone calluses, the number of cells expressing MSI1 and RUNX2 increase over time and the intensity of POSTN expression decreases over time. Within bone calluses, we find the presence of MSI1 expression in mesenchymal stromal cells, osteoblasts, and osteocytes but not in hypertrophic chondrocytes. After 14 days, the expression of MSI1, POSTN, and RUNX2 was significantly correlated. Thus, we conclude that musashi-1 potentially serves in the osteogenic differentiation of mesenchymal stromal cells and bone healing. Therefore, further studies are needed to determine the possibility of musashi-1<inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><msup><mrow></mrow><mo>′</mo></msup></semantics></math></inline-formula>s role as a clinical biomarker of bone healing and therapeutic agent for bone regeneration. |
topic |
Musashi-1 Runx2 periostin mesenchymal stem cells bone healing |
url |
https://www.mdpi.com/1422-0067/22/7/3395 |
work_keys_str_mv |
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