Analysis of anti-bacterial and anti oxidative activity of Azadirachta indica bark using various solvents extracts
Herbal medications have been used for relief of symptoms of disease. Regardless of the great advances observed in current medicine in recent decades, plants still make a significant contribution to health care. An alarming increase in bacterial strains resistant to a number of antimicrobial agents d...
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doaj-3f05444401474a03a919a6c351b366f42020-11-25T00:40:54ZengElsevierSaudi Journal of Biological Sciences1319-562X2017-01-01241111410.1016/j.sjbs.2015.08.006Analysis of anti-bacterial and anti oxidative activity of Azadirachta indica bark using various solvents extractsRaid Al Akeel0Ayesha Mateen1K. Janardhan2V.C. Gupta3Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Saud University, Riyadh, Saudi ArabiaCentral Research Institute of Unani Medicine, Hyderabad 500 038, IndiaP.G. Department of Biotechnology, A.V. College, Hyderabad, A.P., IndiaCentral Research Institute of Unani Medicine, Hyderabad 500 038, IndiaHerbal medications have been used for relief of symptoms of disease. Regardless of the great advances observed in current medicine in recent decades, plants still make a significant contribution to health care. An alarming increase in bacterial strains resistant to a number of antimicrobial agents demands that a renewed effort be made to seek antibacterial agents effective against pathogenic bacteria resistant to or less sensitive to current antibiotics. Anti-bacterial activity of Azadirachta indica stem bark was tested against pathogenic Salmonella paratyphi and Salmonella typhi using various solvent extracts. The in vitro anti-bacterial activity was performed by agar well diffusion method and the results were expressed as the average diameter of zone of inhibition of bacterial growth around the well. The ethanol and methanol extracts showed better anti-bacterial activity with zone of inhibition (20–25 mm) when compared with other tested extracts and standard antibiotic Erythromycin (15 mcg) with zone of inhibition (13–14 mm). Using Fisher’s exact test of significance difference was found between two Salmonella strains sensitivity patterns against tested extracts (P ⩽ 0.035). Extracts of A. indica stem bark also exhibited significant antioxidant activity, thus establishing the extracts as an antioxidant. The results obtained in this study give some scientific support to the A. indica stem bark for further investigation of compounds and in future could be used as drug.http://www.sciencedirect.com/science/article/pii/S1319562X15001825Anti-bacterial activityAnti-oxidant activityAzadirachta indica |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Raid Al Akeel Ayesha Mateen K. Janardhan V.C. Gupta |
spellingShingle |
Raid Al Akeel Ayesha Mateen K. Janardhan V.C. Gupta Analysis of anti-bacterial and anti oxidative activity of Azadirachta indica bark using various solvents extracts Saudi Journal of Biological Sciences Anti-bacterial activity Anti-oxidant activity Azadirachta indica |
author_facet |
Raid Al Akeel Ayesha Mateen K. Janardhan V.C. Gupta |
author_sort |
Raid Al Akeel |
title |
Analysis of anti-bacterial and anti oxidative activity of Azadirachta indica bark using various solvents extracts |
title_short |
Analysis of anti-bacterial and anti oxidative activity of Azadirachta indica bark using various solvents extracts |
title_full |
Analysis of anti-bacterial and anti oxidative activity of Azadirachta indica bark using various solvents extracts |
title_fullStr |
Analysis of anti-bacterial and anti oxidative activity of Azadirachta indica bark using various solvents extracts |
title_full_unstemmed |
Analysis of anti-bacterial and anti oxidative activity of Azadirachta indica bark using various solvents extracts |
title_sort |
analysis of anti-bacterial and anti oxidative activity of azadirachta indica bark using various solvents extracts |
publisher |
Elsevier |
series |
Saudi Journal of Biological Sciences |
issn |
1319-562X |
publishDate |
2017-01-01 |
description |
Herbal medications have been used for relief of symptoms of disease. Regardless of the great advances observed in current medicine in recent decades, plants still make a significant contribution to health care. An alarming increase in bacterial strains resistant to a number of antimicrobial agents demands that a renewed effort be made to seek antibacterial agents effective against pathogenic bacteria resistant to or less sensitive to current antibiotics. Anti-bacterial activity of Azadirachta indica stem bark was tested against pathogenic Salmonella paratyphi and Salmonella typhi using various solvent extracts. The in vitro anti-bacterial activity was performed by agar well diffusion method and the results were expressed as the average diameter of zone of inhibition of bacterial growth around the well. The ethanol and methanol extracts showed better anti-bacterial activity with zone of inhibition (20–25 mm) when compared with other tested extracts and standard antibiotic Erythromycin (15 mcg) with zone of inhibition (13–14 mm). Using Fisher’s exact test of significance difference was found between two Salmonella strains sensitivity patterns against tested extracts (P ⩽ 0.035). Extracts of A. indica stem bark also exhibited significant antioxidant activity, thus establishing the extracts as an antioxidant. The results obtained in this study give some scientific support to the A. indica stem bark for further investigation of compounds and in future could be used as drug. |
topic |
Anti-bacterial activity Anti-oxidant activity Azadirachta indica |
url |
http://www.sciencedirect.com/science/article/pii/S1319562X15001825 |
work_keys_str_mv |
AT raidalakeel analysisofantibacterialandantioxidativeactivityofazadirachtaindicabarkusingvarioussolventsextracts AT ayeshamateen analysisofantibacterialandantioxidativeactivityofazadirachtaindicabarkusingvarioussolventsextracts AT kjanardhan analysisofantibacterialandantioxidativeactivityofazadirachtaindicabarkusingvarioussolventsextracts AT vcgupta analysisofantibacterialandantioxidativeactivityofazadirachtaindicabarkusingvarioussolventsextracts |
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