Nanomechanical DNA Origami pH Sensors
Single-molecule pH sensors have been developed by utilizing molecular imaging of pH-responsive shape transition of nanomechanical DNA origami devices with atomic force microscopy (AFM). Short DNA fragments that can form i-motifs were introduced to nanomechanical DNA origami devices with pliers-like...
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doaj-3f1f1349ee4b41008907a2c925cddfee2020-11-24T21:51:47ZengMDPI AGSensors1424-82202014-10-011410193291933510.3390/s141019329s141019329Nanomechanical DNA Origami pH SensorsAkinori Kuzuya0Ryosuke Watanabe1Yusei Yamanaka2Takuya Tamaki3Masafumi Kaino4Yuichi Ohya5Department of Chemistry and Materials Engineering, Kansai University, 3-3-35 Yamate, Suita, Osaka 564-8680, JapanDepartment of Chemistry and Materials Engineering, Kansai University, 3-3-35 Yamate, Suita, Osaka 564-8680, JapanDepartment of Chemistry and Materials Engineering, Kansai University, 3-3-35 Yamate, Suita, Osaka 564-8680, JapanDepartment of Chemistry and Materials Engineering, Kansai University, 3-3-35 Yamate, Suita, Osaka 564-8680, JapanDepartment of Chemistry and Materials Engineering, Kansai University, 3-3-35 Yamate, Suita, Osaka 564-8680, JapanDepartment of Chemistry and Materials Engineering, Kansai University, 3-3-35 Yamate, Suita, Osaka 564-8680, JapanSingle-molecule pH sensors have been developed by utilizing molecular imaging of pH-responsive shape transition of nanomechanical DNA origami devices with atomic force microscopy (AFM). Short DNA fragments that can form i-motifs were introduced to nanomechanical DNA origami devices with pliers-like shape (DNA Origami Pliers), which consist of two levers of 170-nm long and 20-nm wide connected at a Holliday-junction fulcrum. DNA Origami Pliers can be observed as in three distinct forms; cross, antiparallel and parallel forms, and cross form is the dominant species when no additional interaction is introduced to DNA Origami Pliers. Introduction of nine pairs of 12-mer sequence (5'-AACCCCAACCCC-3'), which dimerize into i-motif quadruplexes upon protonation of cytosine, drives transition of DNA Origami Pliers from open cross form into closed parallel form under acidic conditions. Such pH-dependent transition was clearly imaged on mica in molecular resolution by AFM, showing potential application of the system to single-molecular pH sensors.http://www.mdpi.com/1424-8220/14/10/19329DNA OrigamiDNA NanotechnologyProton DetectionpH Sensorsi-motifAFMnanomechanical devicesSingle-Molecule Sensors |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Akinori Kuzuya Ryosuke Watanabe Yusei Yamanaka Takuya Tamaki Masafumi Kaino Yuichi Ohya |
spellingShingle |
Akinori Kuzuya Ryosuke Watanabe Yusei Yamanaka Takuya Tamaki Masafumi Kaino Yuichi Ohya Nanomechanical DNA Origami pH Sensors Sensors DNA Origami DNA Nanotechnology Proton Detection pH Sensors i-motif AFM nanomechanical devices Single-Molecule Sensors |
author_facet |
Akinori Kuzuya Ryosuke Watanabe Yusei Yamanaka Takuya Tamaki Masafumi Kaino Yuichi Ohya |
author_sort |
Akinori Kuzuya |
title |
Nanomechanical DNA Origami pH Sensors |
title_short |
Nanomechanical DNA Origami pH Sensors |
title_full |
Nanomechanical DNA Origami pH Sensors |
title_fullStr |
Nanomechanical DNA Origami pH Sensors |
title_full_unstemmed |
Nanomechanical DNA Origami pH Sensors |
title_sort |
nanomechanical dna origami ph sensors |
publisher |
MDPI AG |
series |
Sensors |
issn |
1424-8220 |
publishDate |
2014-10-01 |
description |
Single-molecule pH sensors have been developed by utilizing molecular imaging of pH-responsive shape transition of nanomechanical DNA origami devices with atomic force microscopy (AFM). Short DNA fragments that can form i-motifs were introduced to nanomechanical DNA origami devices with pliers-like shape (DNA Origami Pliers), which consist of two levers of 170-nm long and 20-nm wide connected at a Holliday-junction fulcrum. DNA Origami Pliers can be observed as in three distinct forms; cross, antiparallel and parallel forms, and cross form is the dominant species when no additional interaction is introduced to DNA Origami Pliers. Introduction of nine pairs of 12-mer sequence (5'-AACCCCAACCCC-3'), which dimerize into i-motif quadruplexes upon protonation of cytosine, drives transition of DNA Origami Pliers from open cross form into closed parallel form under acidic conditions. Such pH-dependent transition was clearly imaged on mica in molecular resolution by AFM, showing potential application of the system to single-molecular pH sensors. |
topic |
DNA Origami DNA Nanotechnology Proton Detection pH Sensors i-motif AFM nanomechanical devices Single-Molecule Sensors |
url |
http://www.mdpi.com/1424-8220/14/10/19329 |
work_keys_str_mv |
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